scholarly journals Functional characterization and regulation of UCP4 expression by adipokinetic hormone in larva and pupa fat body mitochondria from the beetle Zophobas atratus

2012 ◽  
Vol 1817 ◽  
pp. S45
Author(s):  
M. Slocinska ◽  
N. Antos-Krzeminska ◽  
G. Rosinski ◽  
W. Jarmuszkiewicz
Keyword(s):  
Fat Body ◽  
Functional Characterization ◽  
Adipokinetic Hormone ◽  
Zophobas Atratus

Adipokinetic hormone induces changes in the fat body lipid composition of the beetle Zophobas atratus

Peptides ◽  
2014 ◽  
Vol 58 ◽  
pp. 65-73 ◽  
Author(s):  
Marek Gołębiowski ◽  
Magdalena Cerkowniak ◽  
Aleksandra Urbanek ◽  
Małgorzata Słocińska ◽  
Grzegorz Rosiński ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Fat Body ◽  
Body Lipid ◽  
Adipokinetic Hormone ◽  
Zophobas Atratus

scholarly journals Transcriptome analysis reveals potential function of long non-coding RNAs in 20-hydroxyecdysone regulated autophagy in Bombyx mori

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Huili Qiao ◽  
Jingya Wang ◽  
Yuanzhuo Wang ◽  
Juanjuan Yang ◽  
Bofan Wei ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Target Gene ◽  
Fat Body ◽  
Expression Profiles ◽  
Functional Characterization ◽  
Differentially Expressed ◽  
Post Injection ◽  
Biological Processes ◽  
Potential Target ◽  
Non Coding Rnas

Abstract Background 20-hydroxyecdysone (20E) plays important roles in insect molting and metamorphosis. 20E-induced autophagy has been detected during the larval–pupal transition in different insects. In Bombyx mori, autophagy is induced by 20E in the larval fat body. Long non-coding RNAs (lncRNAs) function in various biological processes in many organisms, including insects. Many lncRNAs have been reported to be potential for autophagy occurrence in mammals, but it has not been investigated in insects. Results RNA libraries from the fat body of B. mori dissected at 2 and 6 h post-injection with 20E were constructed and sequenced, and comprehensive analysis of lncRNAs and mRNAs was performed. A total of 1035 lncRNAs were identified, including 905 lincRNAs and 130 antisense lncRNAs. Compared with mRNAs, lncRNAs had longer transcript length and fewer exons. 132 lncRNAs were found differentially expressed at 2 h post injection, compared with 64 lncRNAs at 6 h post injection. Thirty differentially expressed lncRNAs were common at 2 and 6 h post-injection, and were hypothesized to be associated with the 20E response. Target gene analysis predicted 6493 lncRNA-mRNA cis pairs and 42,797 lncRNA-mRNA trans pairs. The expression profiles of LNC_000560 were highly consistent with its potential target genes, Atg4B, and RNAi of LNC_000560 significantly decreased the expression of LNC_000560 and Atg4B. These results indicated that LNC_000560 was potentially involved in the 20E-induced autophagy of the fat body by regulating Atg4B. Conclusions This study provides the genome-wide identification and functional characterization of lncRNAs associated with 20E-induced autophagy in the fat body of B. mori. LNC_000560 and its potential target gene were identified to be related to 20-regulated autophagy in B. mori. These results will be helpful for further studying the regulatory mechanisms of lncRNAs in autophagy and other biological processes in this insect model.

scholarly journals MicroRNA-8 targets the Wingless signaling pathway in the female mosquito fat body to regulate reproductive processes

2015 ◽  
Vol 112 (5) ◽  
pp. 1440-1445 ◽  
Author(s):  
Keira J. Lucas ◽  
Sourav Roy ◽  
Jisu Ha ◽  
Amanda L. Gervaise ◽  
Vladimir A. Kokoza ◽  
...  
Keyword(s):  
Blood Meal ◽  
Molecular Mechanisms ◽  
Fat Body ◽  
Control Strategies ◽  
Functional Characterization ◽  
Target Prediction ◽  
Underlying Mechanism ◽  
Female Mosquito ◽  
Reproductive Events

Female mosquitoes require a blood meal for reproduction, and this blood meal provides the underlying mechanism for the spread of many important vector-borne diseases in humans. A deeper understanding of the molecular mechanisms linked to mosquito blood meal processes and reproductive events is of particular importance for devising innovative vector control strategies. We found that the conserved microRNA miR-8 is an essential regulator of mosquito reproductive events. Two strategies to inhibit miR-8 function in vivo were used for functional characterization: systemic antagomir depletion and spatiotemporal inhibition using the miRNA sponge transgenic method in combination with the yeast transcriptional activator gal4 protein/upstream activating sequence system. Depletion of miR-8 in the female mosquito results in defects related to egg development and deposition. We used a multialgorithm approach for miRNA target prediction in mosquito 3′ UTRs and experimentally verified secreted wingless-interacting molecule (swim) as an authentic target of miR-8. Our findings demonstrate that miR-8 controls the activity of the long-range Wingless (Wg) signaling by regulating Swim expression in the female fat body. We discovered that the miR-8/Wg axis is critical for the proper secretion of lipophorin and vitellogenin by the fat body and subsequent accumulation of these yolk protein precursors by developing oocytes.

scholarly journals Expansion of the fatty acyl reductase gene family shaped pheromone communication in Hymenoptera

2018 ◽  
Author(s):  
Michal Tupec ◽  
Aleš Buček ◽  
Heiko Vogel ◽  
Václav Janoušek ◽  
Darina Prchalová ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gene Family ◽  
Fat Body ◽  
Functional Characterization ◽  
Acid Methyl Ester ◽  
Fatty Acyl ◽  
Sequencing Analysis ◽  
Pheromone Communication ◽  
Marking Pheromone ◽  
Fatty Acyl Reductase

AbstractThe conserved fatty acyl reductase (FAR) family is involved in biosynthesis of fatty alcohols that serve a range of biological roles. In moths, butterflies (Lepidoptera), and bees (Hymenoptera), FARs biosynthesize fatty alcohol pheromones participating in mate-finding strategies. Using a combination of next-generation sequencing, analysis of transposable elements (TE) in the genomic environment of FAR genes, and functional characterization of FARs from Bombus lucorum, B. lapidarius, and B. terrestris, we uncovered a massive expansion of the FAR gene family in Hymenoptera, presumably facilitated by TEs. Expansion occurred in the common ancestor of bumblebees (Bombini) and stingless bees (Meliponini) after their divergence from the honeybee lineage. We found that FARs from the expanded FAR-A orthology group contributed to the species-specific male marking pheromone composition. Our results indicate that TE-mediated expansion and functional diversification of the FAR gene family played a key role in the evolution of pheromone communication in the crown group of Hymenoptera.AbbreviationsMMP: male marking pheromone, FA: fatty acid, FAME: fatty acid methyl ester, FAR: fatty acyl reductase, LG: labial gland, FB: fat body, TE: transposable element.

scholarly journals Isolation and structure of a novel charged member of the red–pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera)

1990 ◽  
Vol 269 (2) ◽  
pp. 309-313 ◽  
Author(s):  
G Gäde ◽  
H Wilps ◽  
R Kellner
Keyword(s):  
Periplaneta Americana ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Reversed Phase ◽  
American Cockroach ◽  
Red Pigment ◽  
Adipokinetic Hormone ◽  
Migratory Locust ◽  
Corpora Cardiaca ◽  
Adipokinetic Hormone Family

A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named ‘Phormia terraenovae hypertrehalosaemic hormone’ (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.

scholarly journals Localization and Functional Characterization of a Novel Adipokinetic Hormone in the Mollusk, Aplysia californica

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e106014 ◽  
Author(s):  
Joshua I. Johnson ◽  
Scott I. Kavanaugh ◽  
Cindy Nguyen ◽  
Pei-San Tsai
Keyword(s):  
Functional Characterization ◽  
Aplysia Californica ◽  
Adipokinetic Hormone
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