scholarly journals Induction of uncoupling protein UCP3 by hydrogen peroxide increases survival in cardiac muscle cells: Implication of the antioxidant transcription factor Nrf2

2012 ◽  
Vol 1817 ◽  
pp. S40-S41
Author(s):  
A. Anedda ◽  
B. Acosta-Iborra ◽  
E. López-Bernardo ◽  
C. Vaca ◽  
M.O. Landázuri ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Transcription Factor ◽  
Cardiac Muscle ◽  
Uncoupling Protein ◽  
Muscle Cells ◽  
Cardiac Muscle Cells

scholarly journals Inhibition of transcription factor GATA-4 expression blocks in vitro cardiac muscle differentiation.

1995 ◽  
Vol 15 (8) ◽  
pp. 4095-4102 ◽  
Author(s):  
C Grépin ◽  
L Robitaille ◽  
T Antakly ◽  
M Nemer
Keyword(s):  
Transcription Factor ◽  
Cardiac Muscle ◽  
Heart Development ◽  
Muscle Cells ◽  
Cardiac Differentiation ◽  
Marker Genes ◽  
Early Event ◽  
Heart Tube ◽  
Cardiac Muscle Cells ◽  
Cardiac Lineage

Commitment of mesodermal cells to the cardiac lineage is a very early event that occurs during gastrulation, and differentiation of cardiac muscle cells begins in the presomite stage prior to formation of the beating heart tube. However, the molecular events, including gene products that are required for differentiation of cardiac muscle cells, remain essentially unknown. GATA-4 is a recently characterized cardiac muscle-restricted transcription factor whose properties suggest an important regulatory role in heart development. We tested the role of GATA-4 in cardiac differentiation, using the pluripotent P19 embryonal carcinoma cells, which can be differentiated into beating cardiac muscle cells. In this system, GATA-4 transcripts and protein are restricted to cells committed to the cardiac lineage, and induction of GATA-4 precedes expression of cardiac marker genes and appearance of beating cells. Inhibition of GATA-4 expression by antisense transcripts blocks development of beating cardiac muscle cells and interferes with expression of cardiac muscle markers. These data indicate that GATA-4 is necessary for development of cardiac muscle cells and identify for the first time a tissue-specific transcription factor that may be crucial for early steps of mammalian cardiogenesis.

A new serum-responsive, cardiac tissue-specific transcription factor that recognizes the MEF-2 site in the myosin light chain-2 promoter

1993 ◽  
Vol 13 (2) ◽  
pp. 1222-1231
Author(s):  
M D Zhou ◽  
S K Goswami ◽  
M E Martin ◽  
M A Siddiqui
Keyword(s):  
Transcription Factor ◽  
Cardiac Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Cardiac Tissue ◽  
Muscle Cells ◽  
Cardiac Muscle Cells ◽  
Tissue Specific ◽  
Specific Transcription Factor ◽  
Myosin Light Chain 2

We have identified a serum-responsive, cardiac tissue-specific transcription factor, BBF-1, that recognizes an AT-rich sequence (element B), identical to the myocyte enhancer factor (MEF-2) target site, in the cardiac myosin light chain-2 (MLC-2) promoter. Deletion of the element B sequence alone from the cardiac MLC-2 promoter causes, as does that of the MEF-2 site from other promoters and the enhancer of skeletal muscle genes, a marked reduction of transcription. BBF-1 is distinguishable from cardiac MEF-2 on the basis of immunoprecipitation with an antibody which recognizes MEF-2 but not BBF-1. Unlike MEF-2, BBF-1 is present exclusively in nuclear extracts from cardiac muscle cells cultured in a medium containing a high concentration of serum. Removal of serum from culture medium abolishes BBF-1 activity selectively with a concomitant loss of the positive regulatory effect of element B on MLC-2 gene transcription, indicating that there is a correlation between the BBF-1 binding activity and the tissue-specific role of the element B (MEF-2 site) sequence. The loss of element B-mediated activation of transcription is reversed following the refeeding of cells with serum-containing medium. These data demonstrate that cardiac muscle cells contain two distinct protein factors, MEF-2 and BBF-1, which bind to the same target site but that, unlike MEF-2, BBF-1 is serum inducible and cardiac tissue specific. BBF-1 thus appears to be a crucial member of the MEF-2 family of proteins which will serve as an important tool in understanding the regulatory mechanism(s) underlying cardiogenic differentiation.

scholarly journals A new serum-responsive, cardiac tissue-specific transcription factor that recognizes the MEF-2 site in the myosin light chain-2 promoter.

1993 ◽  
Vol 13 (2) ◽  
pp. 1222-1231 ◽  
Author(s):  
M D Zhou ◽  
S K Goswami ◽  
M E Martin ◽  
M A Siddiqui
Keyword(s):  
Transcription Factor ◽  
Cardiac Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Cardiac Tissue ◽  
Muscle Cells ◽  
Cardiac Muscle Cells ◽  
Tissue Specific ◽  
Specific Transcription Factor ◽  
Myosin Light Chain 2

We have identified a serum-responsive, cardiac tissue-specific transcription factor, BBF-1, that recognizes an AT-rich sequence (element B), identical to the myocyte enhancer factor (MEF-2) target site, in the cardiac myosin light chain-2 (MLC-2) promoter. Deletion of the element B sequence alone from the cardiac MLC-2 promoter causes, as does that of the MEF-2 site from other promoters and the enhancer of skeletal muscle genes, a marked reduction of transcription. BBF-1 is distinguishable from cardiac MEF-2 on the basis of immunoprecipitation with an antibody which recognizes MEF-2 but not BBF-1. Unlike MEF-2, BBF-1 is present exclusively in nuclear extracts from cardiac muscle cells cultured in a medium containing a high concentration of serum. Removal of serum from culture medium abolishes BBF-1 activity selectively with a concomitant loss of the positive regulatory effect of element B on MLC-2 gene transcription, indicating that there is a correlation between the BBF-1 binding activity and the tissue-specific role of the element B (MEF-2 site) sequence. The loss of element B-mediated activation of transcription is reversed following the refeeding of cells with serum-containing medium. These data demonstrate that cardiac muscle cells contain two distinct protein factors, MEF-2 and BBF-1, which bind to the same target site but that, unlike MEF-2, BBF-1 is serum inducible and cardiac tissue specific. BBF-1 thus appears to be a crucial member of the MEF-2 family of proteins which will serve as an important tool in understanding the regulatory mechanism(s) underlying cardiogenic differentiation.

THE EFFECTS OF ACUPUNCTURE ON CARDIAC MUSCLE CELLS AND BLOOD PRESSURE IN SPONTANEOUS HYPERTENSIVE RATS

2004 ◽  
Vol 29 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Hung-Chien Wu ◽  
Jaung-Geng Lin ◽  
Chun-Hsien Chu ◽  
Yung-Hsien Chang ◽  
Chung-Gwo Chang ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiac Muscle ◽  
Muscle Cells ◽  
Hypertensive Rats ◽  
Cardiac Muscle Cells

Dendroaspis Natriuretic Peptide Induces the Apoptosis of Cardiac Muscle Cells

2005 ◽  
Vol 27 (1) ◽  
pp. 33-51 ◽  
Author(s):  
Ki-Chan Ha ◽  
Han-Jung Chae ◽  
Cheng-Shi Piao ◽  
Suhn-Hee Kim ◽  
Hyung-Ryong Kim ◽  
...  
Keyword(s):  
Cardiac Muscle ◽  
Natriuretic Peptide ◽  
Muscle Cells ◽  
Cardiac Muscle Cells

Microwave Radiation Effects on Cardiac Muscle Cells in Vitro

1981 ◽  
Vol 86 (2) ◽  
pp. 358 ◽  
Author(s):  
M. J. Galvin ◽  
C. A. Hall ◽  
D. I. McRee
Keyword(s):  
Cardiac Muscle ◽  
Microwave Radiation ◽  
Radiation Effects ◽  
Muscle Cells ◽  
Cardiac Muscle Cells
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