scholarly journals The regulatory role of mitochondria in capacitative calcium entry

2006 ◽  
Vol 1757 (5-6) ◽  
pp. 380-387 ◽  
Author(s):  
Jerzy Duszyński ◽  
Rafał Kozieł ◽  
Wojciech Brutkowski ◽  
Joanna Szczepanowska ◽  
Krzysztof Zabłocki
2005 ◽  
Vol 289 (3) ◽  
pp. C625-C632 ◽  
Author(s):  
Eamonn Bradley ◽  
Mark A. Hollywood ◽  
Noel G. McHale ◽  
Keith D. Thornbury ◽  
Gerard P. Sergeant

The aim of the present study was to investigate the properties and role of capacitative Ca2+ entry (CCE) in interstitial cells (IC) isolated from the rabbit urethra. Ca2+ entry in IC was larger in cells with depleted intracellular Ca2+ stores compared with controls, consistent with influx via a CCE pathway. The nonselective Ca2+ entry blockers Gd3+ (10 μM), La3+ (10 μM), and Ni2+ (100 μM) reduced CCE by 67% ( n = 14), 65% ( n = 11), and 55% ( n = 9), respectively. These agents did not inhibit Ca2+ entry when stores were not depleted. Conversely, CCE in IC was resistant to SKF-96365 (10 μM), wortmannin (10 μM), and nifedipine (1 μM). Spontaneous transient inward currents were recorded from IC voltage-clamped at −60 mV. These events were not significantly affected by Gd3+ (10 μM) or La3+ (10 μM) and were only slightly decreased in amplitude by 100 μM Ni2+. The results from this study demonstrate that freshly dispersed IC from the rabbit urethra possess a CCE pathway. However, influx via this pathway does not appear to contribute to spontaneous activity in these cells.


2001 ◽  
Vol 276 (19) ◽  
pp. 15945-15952 ◽  
Author(s):  
Lisa M. Broad ◽  
Franz-Josef Braun ◽  
Jean-Philippe Lievremont ◽  
Gary St. J. Bird ◽  
Tomohiro Kurosaki ◽  
...  

2008 ◽  
Vol 79 (1) ◽  
pp. 98-109 ◽  
Author(s):  
Paola Pizzo ◽  
Andrea Burgo ◽  
Tullio Pozzan ◽  
Cristina Fasolato

2014 ◽  
Author(s):  
Agnieszka Rak-Mardyla ◽  
Anna Wrobel ◽  
Eliza Drwal ◽  
Ewa Gregoraszczuk

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