Endosomal dysfunction impacts extracellular vesicle release: Central role in Aβ pathology

2020 ◽  
Vol 58 ◽  
pp. 101006 ◽  
Author(s):  
B.D. Arbo ◽  
L.R. Cechinel ◽  
R.P. Palazzo ◽  
I.R. Siqueira
Keyword(s):  
Extracellular Vesicle ◽  
Vesicle Release

scholarly journals Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Albert Lu ◽  
Paulina Wawro ◽  
David W Morgens ◽  
Fernando Portela ◽  
Michael C Bassik ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Mechanisms ◽  
Membrane Vesicles ◽  
Extracellular Vesicle ◽  
Biologically Active ◽  
Vesicle Release ◽  
Guide Rnas ◽  
Disease States ◽  
Genome Wide ◽  
First Time

Extracellular vesicles mediate transfer of biologically active molecules between neighboring or distant cells, and these vesicles may play important roles in normal physiology and the pathogenesis of multiple disease states including cancer. However, the underlying molecular mechanisms of their biogenesis and release remain unknown. We designed artificially barcoded, exosomal microRNAs (bEXOmiRs) to monitor extracellular vesicle release quantitatively using deep sequencing. We then expressed distinct pairs of CRISPR guide RNAs and bEXOmiRs, enabling identification of genes influencing bEXOmiR secretion from Cas9-edited cells. This approach uncovered genes with unrecognized roles in multivesicular endosome exocytosis, including critical roles for Wnt signaling in extracellular vesicle release regulation. Coupling bEXOmiR reporter analysis with CRISPR-Cas9 screening provides a powerful and unbiased means to study extracellular vesicle biology and for the first time, to associate a nucleic acid tag with individual membrane vesicles.

scholarly journals Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors

2019 ◽  
Vol 76 (12) ◽  
pp. 2463-2476 ◽  
Author(s):  
Zsuzsanna Szvicsek ◽  
Ádám Oszvald ◽  
Lili Szabó ◽  
Gyöngyvér Orsolya Sándor ◽  
Andrea Kelemen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Progression ◽  
Extracellular Vesicle ◽  
Vesicle Release ◽  
Intestinal Organoids ◽  
Colorectal Cancer Progression ◽  
Apc Mutation

scholarly journals Cannabidiol Affects Extracellular Vesicle Release, miR21 and miR126, and Reduces Prohibitin Protein in Glioblastoma Multiforme Cells

2019 ◽  
Vol 12 (3) ◽  
pp. 513-522 ◽  
Author(s):  
Uchini S. Kosgodage ◽  
Pinar Uysal-Onganer ◽  
Amy MacLatchy ◽  
Rhys Mould ◽  
Alistair V. Nunn ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Extracellular Vesicle ◽  
Vesicle Release

Molecular Mechanisms of Hypoxia‐Induced Extracellular Vesicle Release

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Silvia Mora ◽  
Ana Muniz-Garcia ◽  
Antonio Zorzano
Keyword(s):  
Molecular Mechanisms ◽  
Extracellular Vesicle ◽  
Vesicle Release

scholarly journals Short-term particulate matter exposure induces extracellular vesicle release in overweight subjects

2017 ◽  
Vol 155 ◽  
pp. 228-234 ◽  
Author(s):  
Matteo Bonzini ◽  
Laura Pergoli ◽  
Laura Cantone ◽  
Mirjam Hoxha ◽  
Andrea Spinazzè ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Extracellular Vesicle ◽  
Short Term ◽  
Vesicle Release ◽  
Particulate Matter Exposure ◽  
Overweight Subjects

scholarly journals Effects of ATP9A on Extracellular Vesicle Release and Exosomal Lipid Composition

2020 ◽  
Vol 2020 ◽  
pp. 1-17
Author(s):  
Xiao Xu ◽  
Limei Xu ◽  
Peng Zhang ◽  
Kan Ouyang ◽  
Yin Xiao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Extracellular Vesicles ◽  
Lipid Composition ◽  
Extracellular Vesicle ◽  
Biological Processes ◽  
Vesicle Release ◽  
Endosomal Recycling ◽  
Intracellular Compartments ◽  
Intercellular Communications

Numerous biological processes are regulated by the intercellular communications arising from extracellular vesicles (EVs) released from cells. However, the mechanisms that regulate the quantity of EV discharged have yet to be understood. While it is known that ATP9A, a P4-ATPase, is involved in endosomal recycling, it is not clear whether it also contributes to the release of EVs and the makeup of exosomal lipids. This study is aimed at exploring the role of human ATP9A in the process of EV release and, further, to analyze the profiles of EV lipids regulated by ATP9A. Our results demonstrate that ATP9A is located in both the intracellular compartments and the plasma membrane. The percentage of ceramides and sphingosine was found to be significantly greater in the control cells than in the ATP9A overexpression and ATP9A knockout groups. However, EV release was greater in ATP9A knockout cells, indicating that ATP9A inhibits the release of EVs. This study revealed the effects of ATP9A on the release of EVs and the lipid composition of exosomes.

scholarly journals RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis

2021 ◽  
Author(s):  
Maria Laura Tognoli ◽  
Nikola Vlahov ◽  
Sander Steenbeek ◽  
Anna M Grawenda ◽  
Michael Eyres ◽  
...  
Keyword(s):  
Extracellular Vesicle ◽  
Vesicle Release ◽  
Cancer Stemness
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