Templated seed-mediated derived Au nanoarchitectures embedded with nanochitosan: Sensitive electrochemical aptasensor for vascular endothelial growth factor and living MCF-7 cell detection

2019 ◽  
Vol 481 ◽  
pp. 505-514 ◽  
Author(s):  
Haiyang Wang ◽  
Yashen Ma ◽  
Chuanpan Guo ◽  
Yongpeng Yang ◽  
Zhikun Peng ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Cell Detection ◽  
Vascular Endothelial ◽  
Electrochemical Aptasensor ◽  
Seed Mediated ◽  
Endothelial Growth Factor ◽  
Mcf 7

scholarly journals Xenoestrogens modulate vascular endothelial growth factor secretion in breast cancer cells through an estrogen receptor-dependent mechanism

2007 ◽  
Vol 196 (2) ◽  
pp. 399-412 ◽  
Author(s):  
Hélène Buteau-Lozano ◽  
Guillaume Velasco ◽  
Monique Cristofari ◽  
Patrick Balaguer ◽  
Martine Perrot-Applanat
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Estrogen Receptor ◽  
Growth Factor ◽  
Breast Cancer Cells ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Endothelial Growth Factor ◽  
Dependent Mechanism ◽  
Mcf 7

Environmental chemicals may affect human health by disrupting endocrine function. Their possible role in the mammary gland and breast tumors is still unknown. Previous studies have demonstrated that vascular endothelial growth factor (VEGF), a key factor in angiogenesis and tumor progression, is an estrogen-regulated gene. We analyzed whether VEGF expression is regulated by different xenoestrogens in several breast cancer cells, MELN (derived from MCF-7) and MELP (derived from MDA-MB-231) and stably expressing estrogen receptor α (ERα); these cell lines stably express estrogen response element (β-globin)-luciferase. Genistein, bisphenol A (BPA), 4-(tert-octyl)phenol (OP), dieldrin, and several phthalates, including benzyl butyl phthalate (BBP) and di-ethyl-2-hexyle phthalate (DEHP), were first shown to be estrogenic. These compounds induced a dose-dependent increase of VEGF secretion in MELN and MCF-7 cells; maximal effect was observed at 1–10 μM non-cytotoxic concentrations and was inhibited by the antiestrogen ICI 182 780. VEGF increase was not observed in ERα-negative MDA-MB-231 cells. Most substances increased VEGF transcript levels in MELN cells. In contrast, γ-hexachlorocyclohexane, vinclozolin, and the phthalates (mono-n-butyl ester phthalic acid, di-isononyle phthalate, and di-isodecyle phthalate) were ineffective on both VEGF secretion and estrogenic luciferase induction in these cell lines. Specific kinase inhibitors PD98059, SB203580, or LY294002 suppressed the xenoestrogen-induced VEGF response, suggesting activation of MEK, p38 kinase, and phosphatidylinositol-3-kinase pathways. Our in vitro results show for the first time that genistein and xenoestrogens (BPA, OP, dieldrin, BBP, and DEHP at high concentrations) up-regulate VEGF expression in MELN cells by an ER-dependent mechanism. Since VEGF increases capillary permeability and breast tumor angiogenesis in vivo, the physiological relevance of these findings is discussed.

scholarly journals The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities

2021 ◽  
Vol 5 (3) ◽  
pp. 669-674
Author(s):  
Muhartono Muhartono ◽  
Asep Sukohar ◽  
Khairun Nisa ◽  
Ratna Dewi Puspita Sari ◽  
Suharyani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ethanolic Extract ◽  
Potential Effect ◽  
Major Public Health Problem ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Vegf Level ◽  
Mcf 7

Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause. Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm. Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells line

scholarly journals The Ethanolic Extract of Annonaceous sp. to Inhibit Vascular Endothelial Growth Factor (VEGF) as Anticancer Modalities

2021 ◽  
Vol 5 (7) ◽  
pp. 642-646
Author(s):  
Muhartono Muhartono ◽  
Asep Sukohar ◽  
Khairun Nisa ◽  
Ratna Dewi Puspita Sari ◽  
Suharyani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Ethanolic Extract ◽  
Potential Effect ◽  
Major Public Health Problem ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Vegf Level ◽  
Mcf 7

Introduction: Cancer has become a major public health problem worldwide, with breast cancer is the most common cancer among women and one of the most important causes of death among them. Management of breast cancer have some adverse effects, with many factorx=s shiouln be considered to ordered the therapy. Use of chemotherapy will suppress cell proliferation and trigger apoptosis, but it cannot just focus on cancer cells only, but also would affected on other cells and cause. Objective: This study aim to analyze the inhibition effect of the Annonanceous sp. ethanolic extract to Vascular Endothelial Growth Factor (VEGF) on MCF-7 cells line Material and Methods: This study is an analytic experimental study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured used RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. were exposed to MCF-7 cells for 48 hours. Analyze of VEGF level use ELISA Methods with λ=405 nm. Results: The results of ELISA analyze shows that the ethanolic extract of Annonaceous sp. have potential effect to decreased of VEGF expression on sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shows the potential effect to decreased of VEGF level on MCF-7 cells line

A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction

2021 ◽  
Author(s):  
Zhong Gao ◽  
Fangling Ren ◽  
Guangyi Yang ◽  
Guangjun Feng ◽  
Lun Wu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
High Sensitivity ◽  
Vascular Endothelial ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Sensitivity Specificity ◽  
Endothelial Growth Factor

An electrochemical aptasensor with high sensitivity, specificity and good intra-day reproducibility is reported to meet the detection needs of vascular endothelial growth factor (VEGF). The toehold-mediated strand displacement recycling amplification...

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