Fabrication of polycaprolactone electrospun fibers with different hierarchical structures mimicking collagen fibrils for tissue engineering scaffolds

Silk fibroin tissue engineering scaffolds with aligned electrospun fibers in multiple layers

RSC Advances ◽

10.1039/c4ra05918b ◽

2014 ◽

Vol 4 (88) ◽

pp. 47570-47575 ◽

Cited By ~ 15

Author(s):

Nannan Jiang ◽

Xiangyu Huang ◽

Zhaobo Li ◽

Lujie Song ◽

Hongsheng Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Silk Fibroin ◽

Electrospun Fibers ◽

Tissue Engineering Scaffolds

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Bicomponent Fibrous Scaffolds of Controlled Composition for Tissue Engineering Applications

Volume 2: Biomedical and Biotechnology Engineering ◽

10.1115/imece2009-10989 ◽

2009 ◽

Author(s):

Jia-Chen Kang ◽

Min Wang ◽

Xiao-Yan Yuan

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Degradation Rate ◽

Therapeutic Agents ◽

Angle Measurement ◽

Electrospun Fibers ◽

Experimental Setup ◽

Scanning Calorimetry ◽

Tissue Engineering Scaffolds ◽

Fibrous Scaffolds

Electrospinning has been widely studied for constructing tissue engineering scaffolds because of the morphological and size effects of electrospun fibers on cell behavior. Research on electrospun tissue engineering scaffolds has been based mainly on using solutions of single polymer or blends of polymers dissolved in common solvents, which has put limitations to scaffolds that can be built. There is an increasing need for using the multi-source and multi-power electrospinning approach to fabricate multicomponent fibrous scaffolds because these scaffolds have great potential for tissue engineering and controlled (drug) release applications. In the present study, bicomponent fibrous scaffolds were fabricated through dual-source and dual-power electrospinning using poly(L-lactic acid) (PLLA) and gelatin polymers. The experimental setup ensured that the solution and electrospinning parameters for each electrospun fibrous component were controlled separately and hence the morphology of electrospun fibers could be controlled and optimized. By adjusting the number of syringes that fed polymer solutions, the composition of bicomponent scaffolds (i.e. the weight percentage of gelatin varying from 0 to 100%) could also be controlled. Such controls would yield scaffolds of desired properties (hydrophilicity, degradation rate, strength, etc.) After electrospinning, pure gelatin scaffolds and bicomponent scaffolds were crosslinked by glutaraldehyde (GA) and genipin, respectively, using different crosslinking methods. Both crosslinked and non-crosslinked scaffolds were studied using various techniques (scanning electron microscopy (SEM) for scaffold morphology, differential scanning calorimetry (DSC) for polymer crystallinity, contact angle measurement for hydrophilicity, tensile testing for mechanical properties and crosslinking efficiency, etc.). It was found that the bicomponent scaffolds were more hydrophilic than pure PLLA scaffolds due to the presence of gelatin fibers. The tensile strength of bicomponent scaffolds was also increased after crosslinking. Using our experimental setup, bicomponent scaffolds could be constructed for tissue engineering with enhanced mechanical properties, biocompatibility and biodegradability. Furthermore, in the bicomponent scaffolds, while PLLA fibers could act as the structural component with a slower degradation rate, the gelatin fibers could be used as a carrier for therapeutic agents (drugs and therapeutic biomolecules). With controlled degrees of the crosslinking of gelatin, the release of therapeutic agents from gelatin fibers would be controlled.

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3D printing of Haversian bone–mimicking scaffolds for multicellular delivery in bone regeneration

Science Advances ◽

10.1126/sciadv.aaz6725 ◽

2020 ◽

Vol 6 (12) ◽

pp. eaaz6725 ◽

Cited By ~ 11

Author(s):

Meng Zhang ◽

Rongcai Lin ◽

Xin Wang ◽

Jianmin Xue ◽

Cuijun Deng ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue ◽

Bone Structure ◽

Hierarchical Structures ◽

Tissue Engineering Scaffolds ◽

Native Bone ◽

And Function

The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, the complexity of hierarchical structures, the requirement for mechanical properties, and the diversity of bone resident cells are the major challenges in constructing biomimetic bone tissue engineering scaffolds. Herein, a Haversian bone–mimicking scaffold with integrated hierarchical Haversian bone structure was successfully prepared via digital laser processing (DLP)–based 3D printing. The compressive strength and porosity of scaffolds could be well controlled by altering the parameters of the Haversian bone–mimicking structure. The Haversian bone–mimicking scaffolds showed great potential for multicellular delivery by inducing osteogenic, angiogenic, and neurogenic differentiation in vitro and accelerated the ingrowth of blood vessels and new bone formation in vivo. The work offers a new strategy for designing structured and functionalized biomaterials through mimicking native complex bone tissue for tissue regeneration.

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Selective HIFU Foaming to Fabricate Porous Polymer for Tissue Engineering Scaffolds

Manufacturing Science and Engineering, Parts A and B ◽

10.1115/msec2006-21043 ◽

2006 ◽

Author(s):

Hai Wang ◽

Wei Li

Keyword(s):

Tissue Engineering ◽

Cellular Response ◽

Focused Ultrasound ◽

Low Cost ◽

Hierarchical Structures ◽

Polymeric Materials ◽

Scanning Speed ◽

High Intensity Focused Ultrasound ◽

Porous Polymer ◽

Tissue Engineering Scaffolds

A novel technique is presented in this paper for the fabrication of tissue engineering scaffolds using the High Intensity Focused Ultrasound (HIFU). This acoustic method is a solvent-free, highly efficient and low cost process that has the potential in scaffold-based tissue engineering. HIFU fabrication technique is capable of creating hierarchically-structured porous polymeric materials, which have various topographical features at different length scales. This will in turn affect the cellular response and behavior of certain type of cells, such as the integration and growth of smooth muscle cells (SMCs). In this study, the effect of HIFU porous polymer fabrication was investigated. Scanning-mode HIFU insonation was performed in the HIFU polymer foaming experiments. The acoustic power and the scanning speed were chosen as the parameters and varied in different groups of experiments. The created microstructures were characterized using the scanning electron microscopy (SEM). The fabricated samples were used for cell culture studies with human aortic SMCs (Passage 4). It was found that the selective HIFU foaming process could be used to create hierarchical structures by choosing appropriate ultrasound parameters. The SMCs were viable on the HIFU-created porous PMMA specimens, and the topographical nature of a HIFU-created porous structure affected the cellular response of SMCs.

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Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

10.1055/s-0039-3400068 ◽

2019 ◽

Author(s):

AS Arampatzis ◽

K Theodoridis ◽

E Aggelidou ◽

KN Kontogiannopoulos ◽

I Tsivintzelis ◽

...

Keyword(s):

Tissue Engineering ◽

Wound Dressings ◽

Active Ingredients ◽

Tissue Engineering Scaffolds

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HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

Dentika Dental Journal ◽

10.32734/dentika.v19i2.457 ◽

2016 ◽

Vol 19 (2) ◽

pp. 93-100

Author(s):

Lalita El Milla

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Functional Groups ◽

Bone Growth ◽

Three Dimensional ◽

Dimensional Structure ◽

Tissue Engineering Scaffolds ◽

Hydroxyapatite Powder ◽

Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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