In vitro study of 3D PLGA/n-HAp/β-TCP composite scaffolds with etched oxygen plasma surface modification in bone tissue engineering

2016 ◽  
Vol 388 ◽  
pp. 321-330 ◽  
Author(s):  
Hee-Sang Roh ◽  
Sang-Chul Jung ◽  
Min-Suk Kook ◽  
Byung-Hoon Kim
Keyword(s):  
Tissue Engineering ◽  
Surface Modification ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Oxygen Plasma ◽  
In Vitro Study ◽  
Vitro Study ◽  
Composite Scaffolds ◽  
Plasma Surface

Three-Dimensionally Printed Polycaprolactone and β-Tricalcium Phosphate Scaffolds for Bone Tissue Engineering: An In Vitro Study

2012 ◽  
Vol 70 (3) ◽  
pp. 647-656 ◽  
Author(s):  
Basel Sharaf ◽  
Caroline B. Faris ◽  
Harutsugi Abukawa ◽  
Srinivas M. Susarla ◽  
Joseph P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
In Vitro Study ◽  
Vitro Study

Fabrication of Pentoxifylline-Loaded Hydroxyapatite/Alginate Scaffold for Bone Tissue Engineering

2020 ◽  
Vol 47 ◽  
pp. 25-40
Author(s):  
Maryam Shadravanan ◽  
Mona Latifi ◽  
Zahra Vojdani ◽  
Tahereh Talaei-Khozani
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Organic Matrix ◽  
Vitro Study ◽  
Calcium Deposition ◽  
Alizarin Red ◽  
Degradation Rates

Background: Hydroxyapatite (HAP), as a common biomaterial in bone tissue engineering, can be fabricated in combination with other osteogenic agents. Pentoxifylline (PTX) is demonstrated to have positive roles in bone defect healing. Since local administration can diminish the systemic side effects of the drug, the objectives of the current in vitro study were to find the effects of PTX on the osteoblast functions for tissue engineering applications. Methods: a HAP scaffold was fabricated by casting the HAP slurry within polyurethane foam. The scaffold was enriched with 5 mg/mL PTX. Alginate (Alg) was used as drug carrier to regulate the PTX releasing rate. MG-63 osteosarcoma cells were cultured on 3D scaffolds and 2D Alg films in the presence or absence of PTX. Results: PTX did not affect the cell viability, attachment and phenotype. Also, the ultrastructure of the scaffolds was not modified by PTX enrichment. Alizarin red S staining showed that PTX has no effect on calcium deposition. Besides, Raman confocal microscopy demonstrated an increase in the organic matrix formation including proline, valine and phenylalanine deposition (represented collagen). Although PTX increased the total protein secretion, it led to a decrease in the alkaline phosphatase activity and vascular endothelial growth factor (VEGF) content. PTX reduced the hydration and degradation rates and it was released mainly at the first 24 hours of incubation. Conclusion: Based on our in vitro study, application of engineered PTX-loaded HAP scaffold in bone regeneration can act on behalf of organic matrix production, but not angiogenesis and mineralization.

scholarly journals In vitro study of novel microparticle based silk fibroin scaffold with osteoblast-like cells for load-bearing osteo-regenerative applications

RSC Advances ◽  
2017 ◽  
Vol 7 (43) ◽  
pp. 26551-26558 ◽  
Author(s):  
Nimisha Parekh ◽  
Chandni Hushye ◽  
Saniya Warunkar ◽  
Sayam Sen Gupta ◽  
Anuya Nisal
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
In Vitro Study ◽  
Vitro Study ◽  
Engineering Applications ◽  
Load Bearing ◽  
Silk Fibroin Scaffold

Silk Fibroin microparticle scaffolds show promise in bone tissue engineering applications.

Bone tissue engineering using 3‐D polycaprolactone/gelatin nanofibrous scaffold containing berberine: In vivo and in vitro study

2021 ◽  
Author(s):  
Arian Ehterami ◽  
Ghasem Abbaszadeh‐Goudarzi ◽  
Saeed Haghi‐Daredeh ◽  
Maryam Niyakan ◽  
Morteza Alizadeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Nanofibrous Scaffold ◽  
Vitro Study

Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study

2020 ◽  
Vol 151 ◽  
pp. 584-594 ◽  
Author(s):  
Muhammad Umar Aslam Khan ◽  
Sajjad Haider ◽  
Saqlain A. Shah ◽  
Saiful Izwan Abd Razak ◽  
Shukur Abu Hassan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nanocomposite Scaffold
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