Bovine serum albumin bioconjugated graphene oxide: Red blood cell adhesion and hemolysis studied by QCM-D

2015 ◽  
Vol 356 ◽  
pp. 844-851 ◽  
Author(s):  
Bing Cai ◽  
Kebang Hu ◽  
Chunming Li ◽  
Jing Jin ◽  
Yuexin Hu
Keyword(s):  
Graphene Oxide ◽  
Cell Adhesion ◽  
Bovine Serum Albumin ◽  
Blood Cell ◽  
Serum Albumin ◽  
Red Blood Cell ◽  
Bovine Serum

In vitro binding of dantrolene to bovine serum albumin and rabbit red blood cell ghosts

1982 ◽  
Vol 60 (10) ◽  
pp. 1307-1311 ◽  
Author(s):  
A. R. Dehpour ◽  
M. Mahmoudian
Keyword(s):  
Bovine Serum Albumin ◽  
Blood Cell ◽  
Serum Albumin ◽  
Red Blood Cell ◽  
Binding Sites ◽  
Blood Cells ◽  
Bovine Serum ◽  
Association Constant ◽  
Dantrolene Sodium

The binding of dantrolene to rabbit red blood cell (RBC) ghosts and bovine serum albumin as models of receptor sites was studied using fluorescence techniques. Dantrolene upon binding to rabbit RBC ghosts and bovine serum albumin showed fluorescence with emission maxima at 495 and 500 nm, respectively. Dantrolene bound to rabbit RBC ghosts with an association constant of 6.06 × 104 M−1 and there were 2.4 × 106 dantrolene binding sites per ghost. The binding of dantrolene to bovine serum albumin showed an anomaly with respect to dantrolene concentration. Dantrolene at concentrations of 1.25 – 3.75 μM bound to bovine serum albumin with an association constant of 1.72 × 104 M−1 and there were 1.078 binding sites/mol bovine serum albumin. The higher affinity of dantrolene toward blood cells was confirmed by studying the distribution of dantrolene between blood cells and plasma after a single i.p. injection of dantrolene sodium (3 mg/kg) to albino rabbits. It was found that the concentration of dantrolene in rabbit blood cells was 2.76 times that of plasma.

Effect of Bovine Serum Albumin on Red Blood Cell Optical Anisotropy Probed Through the Optomechanical Response in an Optical Trap

2017 ◽  
Vol 376 (1) ◽  
pp. 1600209
Author(s):  
Parthasarathi Praveen ◽  
Selvan Rekha ◽  
Devarkonda Chetana ◽  
Shruthi S. Iyengar ◽  
Sarbari Bhattacharya ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Blood Cell ◽  
Serum Albumin ◽  
Red Blood Cell ◽  
Bovine Serum ◽  
Optical Anisotropy ◽  
Optical Trap

Reduced graphene oxide nanosheets for selective picomolar detection of Bovine serum albumin

2021 ◽  
pp. 1-1
Author(s):  
Kunal Biswas ◽  
Avik Sett ◽  
Monojit Mondal ◽  
Srijeet Tripathy ◽  
Jaya Bandyopadhyay ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Reduced Graphene Oxide ◽  
Bovine Serum ◽  
Graphene Oxide Nanosheets ◽  
Reduced Graphene

scholarly journals Grafting Thin Layered Graphene Oxide onto the Surface of Nonwoven/PVDF-PAA Composite Membrane for Efficient Dye and Macromolecule Separations

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 792
Author(s):  
Febri Baskoro ◽  
Selvaraj Rajesh Kumar ◽  
Shingjiang Jessie Lue
Keyword(s):  
Graphene Oxide ◽  
Folic Acid ◽  
Methyl Orange ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Composite Membrane ◽  
Polyacrylic Acid ◽  
Bovine Serum ◽  
Polyvinylidene Fluoride ◽  
Oxide Composite

This study investigates the permeance and rejection efficiencies of different dyes (Rhodamine B and methyl orange), folic acid and a protein (bovine serum albumin) using graphene oxide composite membrane. The ultrathin separation layer of graphene oxide (thickness of 380 nm) was successfully deposited onto porous polyvinylidene fluoride-polyacrylic acid intermediate layer on nonwoven support layer using vacuum filtration. The graphene oxide addition in the composite membrane caused an increased hydrophilicity and negative surface charge than those of the membrane without graphene oxide. In the filtration process using a graphene oxide composite membrane, the permeance values of pure water, dyes, folic acid and bovine serum albumin molecules were more severely decreased (by two orders of magnitude) than those of the nonwoven/polyvinylidene fluoride-polyacrylic acid composite membrane. However, the rejection efficiency of the graphene oxide composite was significantly improved in cationic Rhodamine B (from 9% to 80.3%) and anionic methyl orange (from 28.3% to 86.6%) feed solutions. The folic acid and bovine serum albumin were nearly completely rejected from solutions using either nonwoven/polyvinylidene fluoride-polyacrylic acid or nonwoven/polyvinylidene fluoride-polyacrylic acid/graphene oxide composite membrane, but the latter possessed anti-fouling property against the protein molecules. The separation mechanism in nonwoven/polyvinylidene fluoride-polyacrylic acid membrane includes the Donnan exclusion effect (for smaller-than-pore-size solutes) and sieving mechanism (for larger solutes). The sieving mechanism governs the filtration behavior in the nonwoven/polyvinylidene fluoride-polyacrylic acid/graphene oxide composite membrane.

Controlled Cell Adhesion Using a Biocompatible Anchor for Membrane-Conjugated Bovine Serum Albumin/Bovine Serum Albumin Mixed Layer

Langmuir ◽  
2013 ◽  
Vol 29 (21) ◽  
pp. 6429-6433 ◽  
Author(s):  
Ryuzo Kawamura ◽  
Mari Mishima ◽  
Seunghwan Ryu ◽  
Yu Arai ◽  
Motomu Okose ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Mixed Layer ◽  
Bovine Serum
Sign in / Sign up

Export Citation Format

Share Document