Realignment process of actin stress fibers in single living cells studied by focused femtosecond laser irradiation

2007 ◽  
Vol 253 (15) ◽  
pp. 6416-6419 ◽  
Author(s):  
Ryohei Yasukuni ◽  
Jean-Alexis Spitz ◽  
Rachel Meallet-Renault ◽  
Takayuki Negishi ◽  
Takuji Tada ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Irradiation ◽  
Living Cells ◽  
Stress Fibers ◽  
Femtosecond Laser Irradiation ◽  
Single Living Cells ◽  
Single Living ◽  
Actin Stress Fibers

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Generation of nano-bumps on transparent quartz glass surface under femtosecond laser irradiation

2008 ◽  
Author(s):  
Wei Guo ◽  
Zeng Bo Wang ◽  
Lin Li ◽  
Zhu Liu ◽  
Boris Luk’yanchuk ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Irradiation ◽  
Quartz Glass ◽  
Glass Surface ◽  
Femtosecond Laser Irradiation

Probing nucleus-enriched proteins in single living cells via subcellular-resolved plasmonic immunosandwich assay

The Analyst ◽  
2021 ◽  
Author(s):  
Jia Liu ◽  
Dan Xie ◽  
Zhen Liu
Keyword(s):  
Living Cells ◽  
Nuclear Proteins ◽  
Biological Functions ◽  
Abnormal Expression ◽  
Single Living Cells ◽  
Single Living ◽  
In Cells

Nuclear proteins are crucial in cells and are greatly linked to various biological functions. Abnormal expression of nuclear proteins is associated with many diseases ranging from inflammation to cancer. However,...

scholarly journals Condensation of Si-rich region inside soda-lime glass by parallel femtosecond laser irradiation

2014 ◽  
Vol 22 (13) ◽  
pp. 16493 ◽  
Author(s):  
Masaaki Sakakura ◽  
Kouhei Yoshimura ◽  
Torataro Kurita ◽  
Masahiro Shimizu ◽  
Yasuhiko Shimotsuma ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Laser Irradiation ◽  
Soda Lime ◽  
Femtosecond Laser Irradiation ◽  
Soda Lime Glass ◽  
Rich Region
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