scholarly journals Investigating lipid interactions and the process of raft formation in cellular membranes using ToF-SIMS

2006 ◽  
Vol 252 (19) ◽  
pp. 6716-6718 ◽  
Author(s):  
Carolyn M. McQuaw ◽  
Audra G. Sostarecz ◽  
Leiliang Zheng ◽  
Andrew G. Ewing ◽  
Nicholas Winograd
Keyword(s):  
Cellular Membranes ◽  
Lipid Interactions ◽  
Tof Sims

Identification of a lipid-related peak set to enhance the interpretation of TOF-SIMS data from model and cellular membranes

2011 ◽  
Vol 44 (3) ◽  
pp. 322-333 ◽  
Author(s):  
Christopher R. Anderton ◽  
Bita Vaezian ◽  
Kaiyan Lou ◽  
Jessica F. Frisz ◽  
Mary L. Kraft
Keyword(s):  
Cellular Membranes ◽  
Tof Sims ◽  
Peak Set

Introduction to Cell Membranes at Molecular Resolution

1978 ◽  
Vol 36 (3) ◽  
pp. 497-502
Author(s):  
R.J. Barrnett
Keyword(s):  
Cell Membranes ◽  
International Federation ◽  
Mountain Range ◽  
Cellular Membranes ◽  
Short History ◽  
Biological Organization ◽  
Macromolecular Assemblies ◽  
The Past

This subject, is like observing the panorama of a mountain range, magnificent towering peaks, but it doesn't take much duration of observation to recognize that they are still in the process of formation. The mountains consist of approaches, materials and methods and the rocky substance of information has accumulated to such a degree that I find myself concentrating on the foothills in the foreground in order to keep up with the advance; the edifices behind form a wonderous, substantive background. It's a short history for such an accumulation and much of it has been moved by the members of the societies that make up this International Federation. My panel of speakers are here to provide what we hope is an interesting scientific fare, based on the fact that there is a continuum of biological organization from biochemical molecules through macromolecular assemblies and cellular membranes to the cell itself. Indeed, this fact explains the whole range of towering peaks that have emerged progressively during the past 25 years.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

New approaches to coupling TLC with TOF-SIMS

2005 ◽  
Vol 18 (101) ◽  
pp. 44-50 ◽  
Author(s):  
Andrej Oriňák ◽  
Guido Vering ◽  
Heinrich Arlinghaus ◽  
Jan Andersson ◽  
Ladislav Halas ◽  
...  
Keyword(s):  
New Approaches ◽  
Tof Sims

Optics-Free Visualization of Proteins in Single Cells by Time of Flight-Secondary Ion Mass Spectrometry Coupled with Genetically Encoded Chemical Tags

2020 ◽  
Author(s):  
Feifei Jia ◽  
Jie Wang ◽  
Yanyan Zhang ◽  
Qun Luo ◽  
Luyu Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Single Cells ◽  
Time Of Flight ◽  
E Coli ◽  
Tof Sims ◽  
Ion Mass Spectrometry ◽  
Chemical Tags ◽  
Secondary Ion

<p></p><p><i>In situ</i> visualization of proteins of interest at single cell level is attractive in cell biology, molecular biology and biomedicine, which usually involves photon, electron or X-ray based imaging methods. Herein, we report an optics-free strategy that images a specific protein in single cells by time of flight-secondary ion mass spectrometry (ToF-SIMS) following genetic incorporation of fluorine-containing unnatural amino acids as a chemical tag into the protein via genetic code expansion technique. The method was developed and validated by imaging GFP in E. coli and human HeLa cancer cells, and then utilized to visualize the distribution of chemotaxis protein CheA in E. coli cells and the interaction between high mobility group box 1 protein and cisplatin damaged DNA in HeLa cells. The present work highlights the power of ToF-SIMS imaging combined with genetically encoded chemical tags for <i>in situ </i>visualization of proteins of interest as well as the interactions between proteins and drugs or drug damaged DNA in single cells.</p><p></p>

Studies on a Failure Analysis Flow of Surface Contamination, Corrosion, and Underetch on Microchip Al Bondpads in Wafer Fabrication

2005 ◽  
Author(s):  
Hua Younan
Keyword(s):  
Failure Analysis ◽  
Galvanic Corrosion ◽  
Surface Contamination ◽  
Wafer Fabrication ◽  
Tof Sims ◽  
Corrosion Passivation

Abstract A failure analysis flow is developed for surface contamination, corrosion and underetch on microchip Al bondpads and it is applied in wafer fabrication. SEM, EDX, Auger, FTIR, XPS and TOF-SIMS are used to identify the root causes. The results from carbon related contamination, galvanic corrosion, fluorine-induced corrosion, passivation underetch and Auger bondpad monitoring will be presented. The failure analysis flow will definitely help us to select suitable methods and tools for failure analysis of Al bondpad-related issues, identify rapidly possible root causes of the failures and find the eliminating solutions at both wafer fabrication and assembly houses.

scholarly journals The relationship between protein-protein and protein-lipid interactions and the immunological properties of bovine prothrombin and several of its fragments.

1982 ◽  
Vol 257 (4) ◽  
pp. 1836-1844
Author(s):  
D.A. Madar ◽  
M.M. Sarasua ◽  
H.C. Marsh ◽  
L.G. Pedersen ◽  
K.E. Gottschalk ◽  
...  
Keyword(s):  
Immunological Properties ◽  
Lipid Interactions ◽  
The Relationship
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