scholarly journals Alkaloid profiling of the traditional Chinese medicine Rhizoma corydalis using high performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry

2014 ◽  
Vol 4 (3) ◽  
pp. 208-216 ◽  
Author(s):  
Mingqian Sun ◽  
Jianxun Liu ◽  
Chengren Lin ◽  
Lan Miao ◽  
Li Lin
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
High Performance ◽  
Time Of Flight ◽  
Flight Mass Spectrometry ◽  
Tandem Quadrupole

scholarly journals Rapid Screening of Forskolin-Type Diterpenoids of Blumea aromatica DC Using Ultra-High-Performance Liquid Chromatography Tandem Quadrupole Time-of-Flight Mass Spectrometry Based on the Mass Defect Filtering Approach

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3073
Author(s):  
He ◽  
Zhang ◽  
Yao ◽  
Miao ◽  
Yan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Time Of Flight ◽  
Rapid Screening ◽  
Mass Defect ◽  
Active Compounds ◽  
Flight Mass Spectrometry ◽  
Tandem Quadrupole

The discovery of new active compounds of natural products tends to be increasingly more challenging due to chemical complexity and unpredictable matrices. Forskolin is an active natural labdane-type diterpenoid ingredient widely used worldwide for the treatment of glaucoma, heart failure, hypertension, diabetes, and asthma, and is expected to be a promising anticancer, anti-inflammation, and anti-HIV agent. In recent years, demand for forskolin in the medicine market has increased dramatically. However, natural forskolin originates exclusively from traditional Indian herb medicine Coleus forskohlii (Willd.) Briq. In a previous study, we isolated a series of diterpenoids including an 8,13-epoxy-14ene labdane carbon skeleton from Blumea aromatica DC. In order to identify alternative plant resources, a novel and effective strategy was proposed for the screening of potential forskolin-type diterpenoids (FSKD) compounds obtained from B. aromatica, using the mass defect filtering (MDF) strategy via ultra-high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) approach. Within a narrow, well-defined mass defect range, the strategy developed could significantly improve the detection efficiency of selected FSKD compounds by filtering out certain major or moderate interference compounds. Additionally, the MS/MS cleavage behavior and the characteristic diagnostic ions of the FSKD compounds were proposed to be used in aiding structural identification of the filtration compounds. As a result, a total of 38 FSKD of B. aromatica were filtered out and tentatively identified. To the best of our knowledge, it was the first time that these forskolin-type diterpenoids were identified in B. aromatica, which significantly expands our understanding of the chemical constituents of Blumea species, and allows B. aromatica to be used as a potential alternative plant resource that contains these forskolin-type active compounds. The strategy proposed was proven efficient and reliable for the discovery of novel compounds of herbal extracts.

Ultra-high performance liquid chromatography coupled with time-of-flight mass spectrometry screening and analysis of potential bioactive compounds from traditional chinese medicine Kai-Xin-San, using a multivariate data processing approach and the MetaboLynx tool

RSC Advances ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Chang Liu ◽  
Aihua Zhang ◽  
Ying Han ◽  
Shengwen Lu ◽  
Hui Sun ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Clinical Practice ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Data Processing ◽  
High Performance ◽  
Flight Mass Spectrometry ◽  
Prevention And Treatment

Traditional Chinese Medicine (TCM) has been used in clinical practice for several thousand years, with an indispensable role in prevention and treatment of disease using multiple ingredients.

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