scholarly journals Peer review report 3 on “A technique for marking oncological breast tissue samples in wide local excisions”

2016 ◽  
Vol 5 ◽  
pp. S99
Keyword(s):  
Peer Review ◽  
Breast Tissue ◽  
Tissue Samples

scholarly journals Hormone Receptor Expression Variations in Normal Breast Tissue: Preliminary Results of a Prospective Observational Study

2021 ◽  
Vol 11 (5) ◽  
pp. 387
Author(s):  
Giacomo Santandrea ◽  
Chiara Bellarosa ◽  
Dino Gibertoni ◽  
Maria C. Cucchi ◽  
Alejandro M. Sanchez ◽  
...  
Keyword(s):  
Hormone Receptor ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Receptor Expression ◽  
Hormonal Stimulation ◽  
Tissue Samples ◽  
Hormonal Receptor ◽  
Hormone Receptor Expression ◽  
Cystic Disease Fluid Protein

Normal breast tissue undergoes great variations during a woman’s life as a consequence of the different hormonal stimulation. The purpose of the present study was to examine the hormonal receptor expression variations according to age, menstrual cycle, menopausal state and body mass index. To this purpose, 49 tissue samples of normal breast tissue, obtained during surgery performed for benign and malignant conditions, were immunostained with Estrogen (ER), Progesterone (PR) and Androgen receptors (AR). In addition, Ki67 and Gross Cystic Disease Fluid Protein were studied. The data obtained revealed a great variability of hormone receptor expression. ER and AR generally increased in older and post-menopausal women, while young women presented a higher proliferative rate, evaluated with Ki67. PR increase was observed in women with BMI higher than 25. The different hormonal receptor expression could favor the development of breast cancer.

Angular dispersive diffraction microCT of small breast tissue samples

2008 ◽  
Vol 77 (4) ◽  
pp. 373-380 ◽  
Author(s):  
J.A. Griffiths ◽  
G.J. Royle ◽  
J.A. Horrocks ◽  
A.M. Hanby ◽  
S. Pani ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Tissue Samples ◽  
Small Breast

scholarly journals Association between cadmium and breast cancer

Medicina ◽  
2008 ◽  
Vol 44 (6) ◽  
pp. 415 ◽  
Author(s):  
Loreta Strumylaitė ◽  
Algirdas Boguševičius ◽  
Stanislovas Ryselis ◽  
Darius Pranys ◽  
Lina Poškienė ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Tissue ◽  
Benign Tumor ◽  
Normal Breast Tissue ◽  
Cadmium Concentration ◽  
Normal Breast ◽  
Tissue Samples ◽  
Malignant Breast Tissue ◽  
Two Samples ◽  
Malignant Breast

Cadmium is a known human lung carcinogen, although some studies indicate a link between cadmium exposure and human breast cancer. The objective of this study was to assess cadmium concentration in breast tissue samples of patients with breast cancer and benign breast tumor. Material and methods. The concentration of cadmium was determined in breast tissue samples of 21 breast cancer and 19 benign tumor patients. Two samples of breast tissue from each patient, i.e. tumor and normal tissue close to tumor, were taken for the analysis. Cadmium was determined by atomic absorption spectrometry (Perkin-Elmer, Zeeman 3030). Results. In patients with breast cancer, the mean cadmium concentration was 33.1 ng/g (95% CI, 21.9– 44.4) in malignant breast tissue and 10.4 ng/g (95% CI, 5.6–15.2) in normal breast tissue (P=0.002). In patients with benign tumor, the corresponding values were 17.5 ng/g (95% CI, 8.4–26.5) and 11.8 ng/g (95% CI, 5.1– 18.5) (P=0.3144). There was a statistically significant difference in cadmium concentration between malignant and benign breast tissues (P=0.009). Conclusion. The data obtained show that cadmium concentration is significantly higher in malignant breast tissue as compared with normal breast tissue of the same women or benign breast tissue. Further studies are necessary to determine the association between cadmium concentration in malignant breast tissue and estrogen receptor level, and smoking.

scholarly journals Leptospira in breast tissue and milk of urban Norway rats (Rattus norvegicus)

2016 ◽  
Vol 144 (11) ◽  
pp. 2420-2429 ◽  
Author(s):  
D. DE OLIVEIRA ◽  
C. P. FIGUEIRA ◽  
L. ZHAN ◽  
A. C. PERTILE ◽  
G. G. PEDRA ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Breast Tissue ◽  
Developed Countries ◽  
Immunofluorescence Assay ◽  
Health Concern ◽  
Tissue Samples ◽  
Norway Rat ◽  
First Time ◽  
Globally Distributed ◽  
Norway Rats

SUMMARYLeptospirosis is a zoonosis caused by bacteria of the genus Leptospira. The disease is globally distributed and a major public health concern. The Norway rat (Rattus norvegicus) is the main reservoir of the pathogen in urban slums of developing and developed countries. The potential routes of intra-specific leptospire transmission in rats are largely unknown. Herein, we identified pathogenic Leptospira spp. in breast tissue and milk of naturally infected rats. We examined kidney, breast tissue and milk from 24 lactating rats for the presence of leptospires using immunofluorescence, immunohistochemistry, polymerase chain reaction (PCR) and scanning electronic microscopy. All 24 rats had evidence for Leptospira in the kidneys, indicating chronic carriage. The majority of kidney-positive rats had detectable leptospires in milk (18, 75%) and breast tissue (16, 67%), as evidenced by immunofluorescence assay and immunohistochemistry. Four (17%) milk samples and two (8%) breast tissue samples were positive by quantitative real-time PCR. Scanning electron microscopy confirmed the presence of leptospires in breast tissue. No major pathological changes in breast tissue were found. This study, for the first time, identified leptospires in the milk and breast tissue of wild Norway rats, suggesting the possibility of milk-borne transmission of leptospirosis to neonates.

scholarly journals Invasive breast tumors are characterized by the presence of crystalline nanoparticles

2020 ◽  
Author(s):  
Elena Tsolaki ◽  
William Doran ◽  
Luca Magnani ◽  
Alessandro Olivo ◽  
Inge K. Herrmann ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Diagnosis ◽  
Breast Tissue ◽  
Arterial Wall ◽  
Breast Cancer Diagnosis ◽  
Malignant Tumour ◽  
Spherical Particles ◽  
Tumour Tissue ◽  
Tissue Samples

The presence of calcification in tumours has been known for decades1. Indeed, calcified breast tissue is a fundamental criterion for early breast cancer diagnosis, indicative of malignancies2, and their appearance is used to distinguish between benign and malignant in breast biopsies3,4. However, an in-depth characterization of the nature and origin of tumour tissue calcification remains elusive5–8. Here, we report the presence of nano and micron-sized spherical particles made of highly crystalline whitlockite that are exclusively found in the arterial wall of malignant invasive tumours. By applying nanoanalytical methods to healthy, benign and malignant tumour breast tissue biopsies from patients, we show that poorly crystalline apatite can be found in all breast tissue samples, whereas spherical crystalline whitlockite particles are present only in invasive cancers, mainly in areas close to the lumen of the arterial wall. Moreover, we demonstrate that the concentration of these spherical crystalline particles increases with the grade of disease, and that their size can be related to tumour type. Therefore, our results not only provide new insight into calcification of tumour tissue, but also enable a precise, yet simple route of breast cancer diagnosis and staging.

scholarly journals Structure and Dynamics of the Breast Tissue Microbiomes Under Tumor Influences: An Approach With Neutral, Near-Neutral, and Niche-Neutral Hybrid Models

2021 ◽  
Vol 12 ◽  
Author(s):  
Lianwei Li ◽  
Ping Ning ◽  
Zhanshan Ma
Keyword(s):  
Malignant Tumor ◽  
Breast Tumor ◽  
Breast Tissue ◽  
Benign Tumor ◽  
Hybrid Models ◽  
Bacterial Cells ◽  
Ecological Processes ◽  
Tissue Samples ◽  
Structure And Dynamics ◽  
Diversity Maintenance

The structure and dynamics of breast tissue bacteria can have far-reaching influences on women’s health, particularly on breast tumor development. However, there is little understanding on the ecological processes that shape the structure and dynamics of breast tissue bacteria. Here, we fill the gap by applying three metacommunity models for investigating the community assembly and diversity maintenance, including Sloan near neutral model, Harris et al. multisite neutral and Tang & Zhou niche-neutral hybrid models to reanalyze the 16S-rRNA sequencing datasets of 23 healthy, 12 benign tumor, and 33 malignant tumor tissue samples. First, we found that, at the community/metacommunity levels, the mechanisms of bacteria assembly and diversity maintenance of breast tissue bacteria were moderately influenced by stochastic drifts of bacteria demography (division, death, and dispersal of bacterial cells). At species level, on average, approximately 10 and 5% species were above (positively selected) and below (negatively selected) neutral, respectively. Furthermore, malignant tumor may raise the positively selected species up to 17%. Second, malignant tumor appears to inhibit microbial dispersal as evidenced by lowered migration rates, compared with the migration in normal and benign tumor tissues. These theoretic findings can be inspirational for further investigating the relationships between tissue bacteria and breast tumor progression/development.

scholarly journals Inference of tissue relative proportions of the breast epithelial cell types luminal progenitor, basal, and luminal mature

2021 ◽  
Author(s):  
Thomas E Bartlett ◽  
Swati Chandna ◽  
Sandipan Roy
Keyword(s):  
Progenitor Cells ◽  
Hormone Receptor ◽  
Breast Tissue ◽  
Breast Tumour ◽  
Breast Epithelial Cell ◽  
Fanconi Anaemia ◽  
Breast Cancers ◽  
Luminal Progenitor ◽  
Tissue Samples ◽  
Luminal Progenitor Cells

Hormone receptor negative breast cancers are highly aggressive, and are thought to originate from a subtype of epithelial cells called the luminal progenitor. In this paper, we show how to quantify the number of luminal progenitor cells as well as other epithelial subtypes in breast tissue samples using DNA and RNA based measurements. We find elevated levels of these hormone receptor negative luminal progenitor cells in breast tumour biopsies of hormone receptor negative cancers, as well as in healthy breast tissue samples from BRCA1 (FANCS) mutation carriers. We also find that breast tumours from carriers of heterozygous mutations in non-BRCA Fanconi Anaemia pathway genes are much more likely to be hormone receptor negative.

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