scholarly journals Effects of Dopamine Receptor Type 1 and Gs Protein α Subunit Gene Polymorphisms on Blood Pressure at Rest and in Response to Stress

2006 ◽  
Vol 19 (8) ◽  
pp. 832-836 ◽  
Author(s):  
Y LU ◽  
H ZHU ◽  
X WANG ◽  
H SNIEDER ◽  
Y HUANG ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Dopamine Receptor ◽  
Gene Polymorphisms ◽  
Receptor Type ◽  
Subunit Gene ◽  
Α Subunit ◽  
Response To Stress ◽  
Gs Protein

scholarly journals Constitutive activity of dopamine receptor type 1 (D1R) increases CaV2.2 currents in PFC neurons

2020 ◽  
Vol 152 (5) ◽  
Author(s):  
Clara Inés McCarthy ◽  
Cambria Chou-Freed ◽  
Silvia Susana Rodríguez ◽  
Agustín Yaneff ◽  
Carlos Davio ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
Pyramidal Neurons ◽  
Critical Role ◽  
Intraperitoneal Administration ◽  
Brain Slices ◽  
Physiological Role ◽  
Receptor Type ◽  
Constitutive Activity ◽  
Medial Pfc

Alterations in dopamine receptor type 1 (D1R) density are associated with cognitive deficits of aging and schizophrenia. In the prefrontal cortex (PFC), D1R plays a critical role in the regulation of working memory, which is impaired in these cognitive deficit states, but the cellular events triggered by changes in D1R expression remain unknown. A previous report demonstrated that interaction between voltage-gated calcium channel type 2.2 (CaV2.2) and D1R stimulates CaV2.2 postsynaptic surface location in medial PFC pyramidal neurons. Here, we show that in addition to the occurrence of the physical receptor-channel interaction, constitutive D1R activity mediates up-regulation of functional CaV2.2 surface density. We performed patch-clamp experiments on transfected HEK293T cells and wild-type C57BL/6 mouse brain slices, as well as imaging experiments and cAMP measurements. We found that D1R coexpression led to ∼60% increase in CaV2.2 currents in HEK293T cells. This effect was occluded by preincubation with a D1/D5R inverse agonist, chlorpromazine, and by replacing D1R with a D1R mutant lacking constitutive activity. Moreover, D1R-induced increase in CaV2.2 currents required basally active Gs protein, as well as D1R-CaV2.2 interaction. In mice, intraperitoneal administration of chlorpromazine reduced native CaV currents’ sensitivity to ω-conotoxin-GVIA and their size by ∼49% in layer V/VI pyramidal neurons from medial PFC, indicating a selective effect on CaV2.2. Additionally, we found that reducing D1/D5R constitutive activity correlates with a decrease in the agonist-induced D1/D5R inhibitory effect on native CaV currents. Our results could be interpreted as a stimulatory effect of D1R constitutive activity on the number of CaV2.2 channels available for dopamine-mediated modulation. Our results contribute to the understanding of the physiological role of D1R constitutive activity and may explain the noncanonical postsynaptic distribution of functional CaV2.2 in PFC neurons.

Analysis of mutations in the Gs protein α subunit gene in human leukaemia

1992 ◽  
Vol 16 (5) ◽  
pp. 485-489 ◽  
Author(s):  
Bartrum W. Baker ◽  
John D. Norton
Keyword(s):  
Subunit Gene ◽  
Α Subunit ◽  
Gs Protein ◽  
Human Leukaemia

TNF, TNF receptor type 1, and allograft inflammatory factor-1 gene polymorphisms in Japanese patients with type 1 diabetes

2003 ◽  
Vol 64 (2) ◽  
pp. 302-309 ◽  
Author(s):  
Masataka Nishimura ◽  
Hiroshi Obayashi ◽  
Ikuko Mizuta ◽  
Hirokazu Hara ◽  
Tetsuo Adachi ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Gene Polymorphisms ◽  
Japanese Patients ◽  
Receptor Type ◽  
Inflammatory Factor ◽  
Tnf Receptor

scholarly journals Dopamine D3 receptor gene polymorphisms, blood pressure and nephropathy in type 1 diabetic patients

2004 ◽  
Vol 19 (6) ◽  
pp. 1432-1436 ◽  
Author(s):  
K. J. Pettersson-Fernholm ◽  
C. M. Forsblom ◽  
M. Perola ◽  
J. A. Fagerudd ◽  
P.-H. Groop
Keyword(s):  
Blood Pressure ◽  
Gene Polymorphisms ◽  
Receptor Gene ◽  
Diabetic Patients ◽  
Dopamine D3 Receptor ◽  
D3 Receptor ◽  
Dopamine D3 ◽  
Receptor Gene Polymorphisms ◽  
Type 1 Diabetic Patients

scholarly journals Dopamine receptor type 1 of Caenorhabditis elegans expressing in mechanosensory neurons

2012 ◽  
Vol 28 (1) ◽  
pp. 75-77
Author(s):  
V. V. Stadnyk ◽  
A. Regosh ◽  
C. Y. Mayor ◽  
I. S. Fomenko ◽  
T. I. Bondarchuk ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Dopamine Receptor ◽  
Receptor Type ◽  
Mechanosensory Neurons
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