scholarly journals Platelet-Derived Growth Factor Receptor α Contributes to Human Hepatic Stellate Cell Proliferation and Migration

2017 ◽  
Vol 187 (10) ◽  
pp. 2273-2287 ◽  
Author(s):  
Alexander Kikuchi ◽  
Tirthadipa Pradhan-Sundd ◽  
Sucha Singh ◽  
Shanmugam Nagarajan ◽  
Nick Loizos ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Growth Factor Receptor ◽  
Platelet Derived Growth Factor ◽  
Cell Proliferation And Migration ◽  
Human Hepatic Stellate Cell ◽  
And Migration ◽  
Proliferation And Migration

Requirement of the epidermal growth factor receptor in renal epithelial cell proliferation and migration

2004 ◽  
Vol 287 (3) ◽  
pp. F365-F372 ◽  
Author(s):  
Shougang Zhuang ◽  
Yujing Dang ◽  
Rick G. Schnellmann
Keyword(s):  
Cell Proliferation ◽  
Epidermal Growth Factor Receptor ◽  
Cell Migration ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Mechanical Injury ◽  
Cell Proliferation And Migration ◽  
Epidermal Growth ◽  
And Migration ◽  
Proliferation And Migration

We showed that renal proximal tubular cells (RPTC) can proliferate and migrate following plating and oxidant or mechanical injury in the absence of exogenous growth factors; however, the mechanisms of this response remain unclear. We examined whether epidermal growth factor receptor (EGFR) signaling is activated following plating and mechanical injury and mediates RPTC proliferation and migration. EGFR, Akt [a target of phosphoinositide-3-kinase (PI3K)], and ERK1/2 were activated after plating and mechanical injury, and their phosphorylation was further enhanced by addition of exogenous EGF. Inactivation of the EGFR with the selective inhibitor AG-1478 completely blocked phosphorylation of EGFR, Akt, and ERK1/2 and blocked cell proliferation and migration after plating and injury. Inhibition of PI3K with LY-294002 blocked Akt phosphorylation and proliferation, whereas U-0126 blocked ERK1/2 phosphorylation but had no effect on proliferation. Furthermore, p38 was phosphorylated following mechanical injury and the p38 inhibitor SB-203580 blocked p38 phosphorylation and cell migration. In contrast, neither PI3K nor ERK1/2 inhibition blocked cell migration. These results show that EGFR activation is required for RPTC proliferation and migration and that proliferation is mediated by PI3K, whereas migration is mediated by p38.

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