scholarly journals Interferon-γ Released by Activated CD8+ T Lymphocytes Impairs the Calcium Resorption Potential of Osteoclasts in Calcified Human Aortic Valves

2017 ◽  
Vol 187 (6) ◽  
pp. 1413-1425 ◽  
Author(s):  
Edit Nagy ◽  
Yang Lei ◽  
Eduardo Martínez-Martínez ◽  
Simon C. Body ◽  
Florian Schlotter ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Interferon Γ ◽  
Aortic Valves ◽  
Cd8 T Lymphocytes ◽  
Calcium Resorption

Interferon-γ preferentially reduces memory/effector CD8 T lymphocytes in healthy subjects

1999 ◽  
Vol 134 (2) ◽  
pp. 147-153 ◽  
Author(s):  
J. de Metz ◽  
T.A. Out ◽  
P.C. Wever ◽  
R.M.R. Reijneke ◽  
F. Sprangers ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Healthy Subjects ◽  
Interferon Γ ◽  
Cd8 T Lymphocytes

High Frequencies of Increased Interferon-γ-Producing and/or Skewed CD8+ T Lymphocyte Subfamilies in Patients with Paroxysmal Nocturnal Hemoglobinuria (PNH).

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 980-980
Author(s):  
Akiko Shichishima ◽  
Hideyoshi Noji ◽  
Kazuhiko Ikeda ◽  
Yukio Maruyama ◽  
Tsutomu Shichishima
Keyword(s):  
T Lymphocytes ◽  
T Lymphocyte ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Interferon Γ ◽  
Healthy Individuals ◽  
High Frequencies ◽  
Over Expression ◽  
Cd8 T Lymphocytes ◽  
Cd8 T Lymphocyte ◽  
Ifn Γ

Abstract Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired clonal hematological disorder affecting all hematopoietic lineages, which lack glycosylphosphatidylinositol (GPI)-anchored membrane proteins due to somatic mutations in the phosphatidylinositol glycan-class A gene, and is one disorder of bone marrow failure (BMF) syndromes. Autoreactive T lymphocytes are implicated in some of the immune mechanisms involved in PNH. In fact, we reported recently that the HLA-DRB1*1501 allele and HLA-A*0206 allele is frequent and is related to grading of hemolysis, respectively, in PNH patients (Shichishima T et al, Blood, 2002 and Haematologica, 2006, respectively). However, some characteristics of CD4+ and CD8+ T lymphocytes, including GPI-negative CD4+ and CD8+ T lymphocytes, in PNH patients remain unknown. To know some characteristics of CD4+ and CD8+ T lymphocytes with and without expressions of GPI proteins in PNH, we examined preferential variable beta chain (Vβ) repertoires of the T-cell receptor (TCR) and expressions of interferon-γ (IFN-γ) by flow cytometry and the TCR Vβ complementarity-determining region 3 (CDR3) spectratypes by genetic methods at the same time in CD4+CD59+, CD4+CD59−, CD8+CD59+, and/or CD8+CD59− T lymphocytes from 10 Japanese patients, including 6 and 4 with the HLA-DRB1*1501 allele and HLA-A*0206 allele, respectively, and from 5 age-matched healthy individuals. In the analyses of TCR Vβ repertoires, over-expressed TCR Vβ subfamilies were found in any T lymphocytes subsets from all the patients. We found significantly higher numbers (mean ± standard deviation; 1.9 ± 1.2) of over-expressed TCR Vβ subfamilies in CD8+CD59+ T lymphocytes from PNH patients compared with those (0 ± 0, p <0.01) from healthy individuals. In the TCR Vβ CDR3 spectratyping, skewed TCR Vβ CDR3 spectatypes were found in more than one TCR Vβ subfamilies of CD3+CD4+CD59−, CD3+CD8+CD59+, and CD3+CD8+CD59− T lymphocytes from all the PNH patients. The numbers of skewed TCR Vβ CDR3 spectatypes in CD3+CD8+CD59+ (4.0 ± 3.3) and CD3+CD8+CD59− (7.5 ± 3.9) T lymphocytes from one PNH patient were significantly greater than those in CD3+CD4+CD59+ T lymphocytes (0.6 ± 1.0, p <0.005) and CD3+CD4+CD59− (2.5 ± 1.5, p <0.002), respectively. Skewed TCR Vβ CDR3 spectatypes were found commonly in Vβ 25 subfamily of CD3+CD8+CD59+ and CD3+CD8+CD59− T lymphocytes from all of 4 PNH patients with the HLA-A*0206 allele. In the analyses of IFN-γ expressions, more than one TCR Vβ subfamiliy with over-expression of IFN-γ was found in CD8+CD59+ and/or CD8+CD59− T lymphocytes from 9 patients and in CD4+CD59+ and/or CD4+CD59− T lymphocytes from 8 patients. The numbers of TCR Vβ subfamiliy with over-expression of IFN-γ in CD8+CD59+ T lymphocytes (5.2 ± 4.3), but not in the other T lymphocyte subsets, from one PNH patient were significantly greater than those from healthy individuals (0 ± 0, p <0.05). However, there were no specific Vβ subfamilies determined by any analyses, described above, in PNH. In conclusion, we found high frequencies of increased IFN-γ-producing and/or skewed CD8+ T lymphocyte subfamilies with and/or without CD59 expression in PNH patients, suggesting that these cells may contribute to the occurrence of BMF rather than negative selection of PNH clones through action of IFN-γ in PNH.

Interferon-γ production by CMV-specific CD8+ T lymphocytes provides protection against cytomegalovirus reactivation in critically ill patients

2015 ◽  
Vol 42 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Juan José Castón ◽  
Sara Cantisán ◽  
Francisco González-Gasca ◽  
Aurora Páez-Vega ◽  
Hasania Abdel-Hadi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Interferon Γ ◽  
Cytomegalovirus Reactivation ◽  
Cd8 T Lymphocytes

Interferon-γ secretion and perforin expression are impaired in CD8+ T lymphocytes from patients with undifferentiated carcinoma of nasopharyngeal type

2003 ◽  
Vol 52 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Stefania Zanussi ◽  
Emanuela Vaccher ◽  
Cristina Caffau ◽  
Chiara Pratesi ◽  
Cinzia Crepaldi ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Interferon Γ ◽  
Undifferentiated Carcinoma ◽  
Cd8 T Lymphocytes ◽  
Perforin Expression
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