scholarly journals Impaired Expansion of Regulatory T Cells in a Neonatal Thymectomy-Induced Autoimmune Mouse Model

2015 ◽  
Vol 185 (11) ◽  
pp. 2886-2897 ◽  
Author(s):  
Akiko Yamada ◽  
Aya Ushio ◽  
Rieko Arakaki ◽  
Takaaki Tsunematsu ◽  
Yasusei Kudo ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Neonatal Thymectomy ◽  
Autoimmune Mouse

scholarly journals CD4+CD25+Foxp3+ regulatory T cells regulate immune balance in unexplained recurrent spontaneous abortion via the Toll-like receptor 4/nuclear factor-κB pathway

2020 ◽  
Vol 48 (12) ◽  
pp. 030006052098094
Author(s):  
Shuang Qin ◽  
Li Li ◽  
Jia Liu ◽  
Jinrui Zhang ◽  
Qing Xiao ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Inflammatory Response ◽  
Mouse Model ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
Tlr4 Antagonist

Objective The present study aimed to evaluate the effects of cluster of differentiation (CD)4+CD25+ forkhead box p3 (Foxp3)+ regulatory T cells (Tregs) on unexplained recurrent spontaneous abortion (URSA) and the associated mechanisms. Methods The proportion of CD4+CD25+Foxp3+ Tregs and inflammatory cytokine concentrations in the peripheral blood of women with URSA were measured by flow cytometry and enzyme-linked immunosorbent assay, respectively. CBA/JxDBA/2J mating was used to establish an abortion-prone mouse model and the model mice were treated with the Toll-like receptor 4 (TLR4) antagonist E5564 and the TLR4 agonist lipopolysaccharide. Results The proportion of CD4+CD25+Foxp3+ Tregs was decreased and the inflammatory response was increased in women with URSA. In the abortion-prone mouse model, E5564 significantly increased the proportion of CD4+CD25+Foxp3+ Tregs, decreased the inflammatory response, and increased Foxp3 mRNA and protein expression. Lipopolysaccharide had adverse effects on the abortion-prone model. Conclusions These data suggest that CD4+CD25+Foxp3+ Tregs regulate immune homeostasis in URSA via the TLR4/nuclear factor-κB pathway, and that the TLR4 antagonist E5564 may be a novel and potential drug for treating URSA.

scholarly journals 873 Pharmacologic macrophage depletion affects metastasis formation by modulating systemic immune responses in a genetic pancreatic cancer model

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A926-A926
Author(s):  
Heidi Griesmann ◽  
Heidi Griesmann ◽  
Heidi Griesmann ◽  
Christof Drexel ◽  
Nada Milosevic ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
T Cells ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Immune Cells ◽  
Tumour Progression ◽  
Metastasis Formation ◽  
Macrophage Depletion ◽  
Genetic Mouse Model ◽  
Liposomal Clodronate

BackgroundTumour-associated macrophages (TAM) play an important role in mediating tumour progression. In pancreatic cancer, infiltrating macrophages have been identified not only in invasive tumours, but also in early preinvasive pancreatic intraepithelial neoplasias and are known to mediate tumour progression.MethodsWe aimed to study the impact of pharmacological macrophage depletion by liposomal clodronate in the genetic mouse model of pancreatic cancer (KPC mouse: LSL-KrasG12D/+;LSL-Trp53R172H/+;Pdx-1-Cre). KPC mice were treated with liposomal clodronate or control liposomes from week 8 to week 20. Tumour and metastasis formation as well as alterations in local and circulating immune cells and cytokines were analysed.ResultsTreatment with liposomal clodronate effectively reduced CD11b-positive macrophages both in the pancreas and other organs such as liver, lung and spleen. Tumour incidence and size was only slightly reduced. However, metastasis formation in the liver und lungs was markedly diminished after macrophage depletion. Reduced macrophage count was associated with significant alterations in circulating growth factors and mediators known to be secreted by macrophages and associated with angiogenesis, most prominently VEGF. Moreover, application of liposomal clodronate led to marked alterations in circulating immune cells, among them reduced regulatory T cells.ConclusionsPharmacological depletion of macrophages in a genetic mouse model of pancreatic cancer markedly reduced metastasis formation and is associated with modulated profile of both secreted mediators and regulatory T cells. Pharmacological modulation of infiltrating macrophages represents a promising avenue for antimetastatic therapeutic approaches.

scholarly journals Regulatory T cells exhibit neuroprotective effect in a mouse model of traumatic brain injury

2016 ◽  
Vol 14 (6) ◽  
pp. 5556-5566 ◽  
Author(s):  
Yunhu Yu ◽  
Fang Cao ◽  
Qishan Ran ◽  
Xiaochuan Sun
Keyword(s):  
Traumatic Brain Injury ◽  
T Cells ◽  
Brain Injury ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Neuroprotective Effect

Allergen-specific immunotherapy induces regulatory T cells in an atopic dermatitis mouse model

Allergy ◽  
2018 ◽  
Vol 73 (9) ◽  
pp. 1801-1811 ◽  
Author(s):  
J. U. Shin ◽  
S. H. Kim ◽  
J. Y. Noh ◽  
J. H. Kim ◽  
H. R. Kim ◽  
...  
Keyword(s):  
T Cells ◽  
Atopic Dermatitis ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Specific Immunotherapy ◽  
Allergen Specific Immunotherapy

Immune stimulatory antigen loaded particles combined with depletion of regulatory T-cells induce potent tumor specific immunity in a mouse model of melanoma

2008 ◽  
Vol 58 (4) ◽  
pp. 517-530 ◽  
Author(s):  
Robin Goforth ◽  
Aliasger K. Salem ◽  
Xiaoyan Zhu ◽  
Suzanne Miles ◽  
Xue-Qing Zhang ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Specific Immunity ◽  
Potent Tumor

scholarly journals Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sija Landman ◽  
Vivian L. de Oliveira ◽  
Piet E. J. van Erp ◽  
Esther Fasse ◽  
Stijn C. G. Bauland ◽  
...  
Keyword(s):  
T Cells ◽  
Regulatory T Cells ◽  
Mouse Model ◽  
Low Dose ◽  
Skin Inflammation ◽  
Intradermal Injection ◽  
Humanized Mouse ◽  
Humanized Mouse Model
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