scholarly journals Distinct Renal Pathology and a Chemotactic Phenotype after Enterohemorrhagic Escherichia coli Shiga Toxins in Non-Human Primate Models of Hemolytic Uremic Syndrome

2013 ◽  
Vol 182 (4) ◽  
pp. 1227-1238 ◽  
Author(s):  
Deborah J. Stearns-Kurosawa ◽  
Sun-Young Oh ◽  
Rama P. Cherla ◽  
Moo-Seung Lee ◽  
Vernon L. Tesh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Enterohemorrhagic Escherichia Coli ◽  
Renal Pathology ◽  
Shiga Toxins ◽  
Uremic Syndrome ◽  
Human Primate

Enterohemorrhagic Escherichia coli associated with hemolytic-uremic syndrome in Chilean children.

1992 ◽  
Vol 30 (8) ◽  
pp. 2153-2157 ◽  
Author(s):  
A Cordovéz ◽  
V Prado ◽  
L Maggi ◽  
J Cordero ◽  
J Martinez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Enterohemorrhagic Escherichia Coli ◽  
Uremic Syndrome

scholarly journals Enterohemorrhagic Escherichia coli as Causes of Hemolytic Uremic Syndrome in the Czech Republic

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73927 ◽  
Author(s):  
Monika Marejková ◽  
Květa Bláhová ◽  
Jan Janda ◽  
Angelika Fruth ◽  
Petr Petráš
Keyword(s):  
Escherichia Coli ◽  
Czech Republic ◽  
Hemolytic Uremic Syndrome ◽  
Enterohemorrhagic Escherichia Coli ◽  
The Czech Republic ◽  
Uremic Syndrome

Cytokine profiles of patients with enterohemorrhagic Escherichia coli O111-induced hemolytic-uremic syndrome

Cytokine ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 694-700 ◽  
Author(s):  
Masaki Shimizu ◽  
Mondo Kuroda ◽  
Natsumi Sakashita ◽  
Michio Konishi ◽  
Hisashi Kaneda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Enterohemorrhagic Escherichia Coli ◽  
Cytokine Profiles ◽  
Uremic Syndrome

scholarly journals Identification of Antibiotics That Diminish Disease in a Murine Model of Enterohemorrhagic Escherichia coli Infection

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Sabrina Mühlen ◽  
Isabell Ramming ◽  
Marina C. Pils ◽  
Martin Koeppel ◽  
Jana Glaser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Content Type ◽  
Shiga Toxins ◽  
Escherichia Coli Infection ◽  
Uremic Syndrome ◽  
Severity Of The Disease ◽  
Selection Of ◽  
Mild Diarrhea

ABSTRACT Infections with enterohemorrhagic Escherichia coli (EHEC) cause disease ranging from mild diarrhea to hemolytic-uremic syndrome (HUS) and are the most common cause of renal failure in children in high-income countries. The severity of the disease derives from the release of Shiga toxins (Stx). The use of antibiotics to treat EHEC infections is generally avoided, as it can result in increased stx expression. Here, we systematically tested different classes of antibiotics and found that their influence on stx expression and release varies significantly. We assessed a selection of these antibiotics in vivo using the Citrobacter rodentium ϕstx2dact mouse model and show that stx2d-inducing antibiotics resulted in weight loss and kidney damage despite clearance of the infection. However, several non-Stx-inducing antibiotics cleared bacterial infection without causing Stx-mediated pathology. Our results suggest that these antibiotics might be useful in the treatment of EHEC-infected human patients and decrease the risk of HUS development.

scholarly journals Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Sunao Iyoda ◽  
Shannon D. Manning ◽  
Kazuko Seto ◽  
Keiko Kimata ◽  
Junko Isobe ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Clinical Presentation ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Nitric Oxide Reductase ◽  
Asymptomatic Carriers ◽  
Uremic Syndrome ◽  
Risk Of Disease

Abstract Background.  Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC O157:H7 strains have exhibited high genetic variability, their abilities to cause human diseases have not been fully examined. Methods.  Clade typing and stx subtyping of EHEC O157:H7 strains, which were isolated in Japan during 1999–2011 from 269 HUS patients and 387 asymptomatic carriers (ACs) and showed distinct pulsed-field gel electrophoresis patterns, were performed to determine relationships between specific lineages and clinical presentation. Results.  Clades 6 and 8 strains were more frequently found among the isolates from HUS cases than those from ACs (P = .00062 for clade 6, P < .0001 for clade 8). All clade 6 strains isolated from HUS patients harbored stx2a and/or stx2c, whereas all clade 8 strains harbored either stx2a or stx2a/stx2c. However, clade 7 strains were predominantly found among the AC isolates but less frequently found among the HUS isolates, suggesting a significant association between clade 7 and AC (P < .0001). Logistic regression analysis revealed that 0–9 year old age is a significant predictor of the association between clade 8 and HUS. We also found an intact norV gene, which encodes for a nitric oxide reductase that inhibits Shiga toxin activity under anaerobic condition, in all clades 1–3 isolates but not in clades 4–8 isolates. Conclusions.  Early detection of EHEC O157:H7 strains that belonged to clades 6/8 and harbored specific stx subtypes may be important for defining the risk of disease progression in EHEC-infected 0- to 9-year-old children.

scholarly journals Genomic Comparison of Two O111:H−Enterohemorrhagic Escherichia coli Isolates from a Historic Hemolytic-Uremic Syndrome Outbreak in Australia

2016 ◽  
Vol 84 (3) ◽  
pp. 775-781 ◽  
Author(s):  
Lauren J. McAllister ◽  
Stephen J. Bent ◽  
Nicola K. Petty ◽  
Elizabeth Skippington ◽  
Scott A. Beatson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Hemolytic Uremic Syndrome ◽  
Sequence Data ◽  
Enterohemorrhagic Escherichia Coli ◽  
Nucleotide Polymorphisms ◽  
Extra Copy ◽  
Content Type ◽  
Shiga Toxin 2 ◽  
Uremic Syndrome ◽  
Insertion Sites

EnterohemorrhagicEscherichia coli(EHEC) is an important cause of diarrhea and hemolytic-uremic syndrome (HUS) worldwide. Australia's worst outbreak of HUS occurred in Adelaide in 1995 and was one of the first major HUS outbreaks attributed to a non-O157 Shiga-toxigenicE. coli(STEC) strain. Molecular analyses conducted at the time suggested that the outbreak was caused by an O111:H−clone, with strains from later in the outbreak harboring an extra copy of the genes encoding the potent Shiga toxin 2 (Stx2). Two decades later, we have used next-generation sequencing to compare two isolates from early and late in this important outbreak. We analyzed genetic content, single-nucleotide polymorphisms (SNPs), and prophage insertion sites; for the latter, we demonstrate how paired-end sequence data can be leveraged to identify such insertion sites. The two strains are genetically identical except for six SNP differences and the presence of not one but two additional Stx2-converting prophages in the later isolate. Isolates from later in the outbreak were associated with higher levels of morbidity, suggesting that the presence of the additional Stx2-converting prophages is significant in terms of the virulence of this clone.

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