Utilizing microphysiological systems and induced pluripotent stem cells for disease modeling: a case study for blood brain barrier research in a pharmaceutical setting

2019 ◽  
Vol 140 ◽  
pp. 129-135 ◽  
Author(s):  
Kristin M. Fabre ◽  
Louise Delsing ◽  
Ryan Hicks ◽  
Nicola Colclough ◽  
Damian C. Crowther ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Disease Modeling ◽  
Brain Barrier ◽  
Blood Brain ◽  
Microphysiological Systems ◽  
Induced Pluripotent

scholarly journals Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 994 ◽  
Author(s):  
Annalise Di Marco ◽  
Domenico Vignone ◽  
Odalys Gonzalez Paz ◽  
Ivan Fini ◽  
Maria Rosaria Battista ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Human Model ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Induced Pluripotent

The blood-brain barrier (BBB) is responsible for the homeostasis between the cerebral vasculature and the brain and it has a key role in regulating the influx and efflux of substances, in healthy and diseased states. Stem cell technology offers the opportunity to use human brain-specific cells to establish in vitro BBB models. Here, we describe the establishment of a human BBB model in a two-dimensional monolayer culture, derived from human induced pluripotent stem cells (hiPSCs). This model was characterized by a transendothelial electrical resistance (TEER) higher than 2000 Ω∙cm2 and associated with negligible paracellular transport. The hiPSC-derived BBB model maintained the functionality of major endothelial transporter proteins and receptors. Some proprietary molecules from our central nervous system (CNS) programs were evaluated revealing comparable permeability in the human model and in the model from primary porcine brain endothelial cells (PBECs).

scholarly journals Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Emma K. Hollmann ◽  
Amanda K. Bailey ◽  
Archit V. Potharazu ◽  
M. Diana Neely ◽  
Aaron B. Bowman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Induced Pluripotent Stem Cells ◽  
Blood Brain Barrier ◽  
Pluripotent Stem Cells ◽  
Brain Barrier ◽  
Blood Brain ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document