scholarly journals Three-dimensional cell culture technique and pathophysiology

2014 ◽  
Vol 74 ◽  
pp. 95-103 ◽  
Author(s):  
Michiya Matsusaki ◽  
Charles Patrick Case ◽  
Mitsuru Akashi
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Culture Technique ◽  
Dimensional Cell ◽  
Cell Culture Technique

scholarly journals Cytotoxic Evaluation Using Murine Fibroblasts (L-929) Three Dimensional Cell Culture Technique

2018 ◽  
Vol 14 (1) ◽  
pp. 18-27
Author(s):  
Pratyusha Ghanta ◽  
Upendra Bhatnagar ◽  
Sasidhar Pasumarthi ◽  
Sunil Kumar Chiliveri
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Culture Technique ◽  
Murine Fibroblasts ◽  
Dimensional Cell ◽  
Cell Culture Technique

A Review of the Three-Dimensional Cell Culture Technique: Approaches, Advantages and Applications

2016 ◽  
Vol 11 (4) ◽  
pp. 370-380 ◽  
Author(s):  
Weijie Zhang ◽  
Ai Zhuang ◽  
Ping Gu ◽  
Huifang Zhou ◽  
Xianqun Fan
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Culture Technique ◽  
Dimensional Cell ◽  
Cell Culture Technique

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

A novel fluffy hydroxylapatite fiber scaffold with deep interconnected pores designed for three-dimensional cell culture

2014 ◽  
Vol 2 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Lin Jin ◽  
Zhang-Qi Feng ◽  
Ting Wang ◽  
Zhuozhuo Ren ◽  
Shuangshuang Ma ◽  
...  
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
Interconnected Pores ◽  
Dimensional Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

A Cell Culture Technique for Chromosome Preparation in Cyprinid Fishes

Copeia ◽  
1984 ◽  
Vol 1984 (1) ◽  
pp. 232 ◽  
Author(s):  
Chris T. Amemiya ◽  
John W. Bickham ◽  
John R. Gold
Keyword(s):  
Cell Culture ◽  
Culture Technique ◽  
Chromosome Preparation ◽  
A Cell ◽  
Cyprinid Fishes ◽  
Cell Culture Technique

Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1177-1180 ◽  
Author(s):  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Yuki Shirosaki ◽  
T. Okayama ◽  
K. Kataoka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Pore Diameter ◽  
Sol Gel ◽  
Three Dimensional ◽  
Culture Methods ◽  
Organically Modified ◽  
Dimensional Cell

Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.

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