Desired and side effects of the supplementation with l -glutamine and l -glutathione in enteric glia of diabetic rats

2016 ◽  
Vol 118 (6) ◽  
pp. 625-631 ◽  
Author(s):  
Cynthia Priscilla do Nascimento Bonato Panizzon ◽  
Jacqueline Nelisis Zanoni ◽  
Catchia Hermes-Uliana ◽  
Aline Rosa Trevizan ◽  
Camila Caviquioli Sehaber ◽  
...  
Keyword(s):  
Side Effects ◽  
Diabetic Rats ◽  
Enteric Glia

scholarly journals Effects of Securigera Securidaca Seed Extract, Alone and in combination with Glibenclamide, on Circulating Levels of Pro-Angiogenic/Anti-Angiogenic Biomarkers in Hyperglycemic Rats

2020 ◽  
Author(s):  
Shahin Alizadeh-Fanalou ◽  
Mohammad Babaei ◽  
Elham Bahreini
Keyword(s):  
Side Effects ◽  
Combination Therapy ◽  
Diabetes Complications ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Angiogenic Factors ◽  
Herbal Extract ◽  
Diabetic Patients ◽  
Angiogenic Biomarkers ◽  
Securigera Securidaca

Abstract Background: Many diabetic patients use herbal medicines in addition to their mainstream treatments. Plants contain a well-known and unknown set of compounds that may exacerbate or improve diabetes complications. Thus, the side effects of these herbs should be known before prescribing. The aim of the study is to investigate the effects of hydroalcoholic extract of Securigera securidaca (L.) Degen & Dorfl (S. securidaca) seed (HESS) on angiogenesis/anti-angiogenesis balance in Streptozotocin (STZ)-induced diabetic rats, alone and in combination with glibenclamide. Methods: The groups involved in this animal study included diabetic and healthy control groups, groups treated with three doses of HESS, group treated with glibenclamide, and groups received combination therapy. Serum samples were taken and analyzed for the levels of angiogenic/ anti-angiogenic biomarkers.Results: Induction of diabetes increased serum levels of angiogenic agents and decreased circulating anti-angiogenic factors. The herbal extract, even with the highest dose, had little effects on the blood levels of the tested biomarkers except with TGF-β. Glibenclamide was more effective than the highest dose of HESS in preventing the increase in serum levels of angiogenic factors and in inhibiting the decrease in anti-angiogenic agents in diabetic rats. Combination therapy with the highest dose of HESS partly enhanced the glibenclamide effects.Conclusions: Although glibenclamide was more effective than the highest dose of HESS used in this study in preventing changes in serum concentrations of angiogenic/ anti-angiogenic biomarkers in the diabetic animals, this study show that S. securidaca has no side effects on diabetes complications caused by vascular disorders and neovascularization, and still it can be used as a herbal supplement with the standard drug.

scholarly journals EFFECT OF TETRAHYDROCURCUMIN IN STREPTOZOTOCIN - NICOTINAMIDE INDUCED DIABETES

2021 ◽  
Vol 21 (No 1) ◽  
Author(s):  
Pidaran Murugan
Keyword(s):  
Type 2 Diabetes ◽  
Insulin Secretion ◽  
Side Effects ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Active Metabolites ◽  
Pancreatic Cell ◽  
Major Health Problem ◽  
Chronic Hyperglycemia

Diabetes is a major health problem affecting major populations worldwide. It is characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycemia and disturbances of carbohydrate, lipid and protein metabolism. As a consequence of the metabolic derangements in diabetes, various complications develop including both macro and micro-vascular dysfunctions. Pancreatic cell dysfunction and insulin resistance are the two hallmarks of type 2 diabetes mellitus. Treatment of diabetes without any side effects is still a challenge to the medical system. There is an increasing demand by patients to use the natural products with antidiabetic activity, because insulin and oral hypoglycemic drugs are having so many side effects. Streptozotocin (STZ)-nicotinamide type 2 model shares a number of features with human type 2 diabetes and is characterized by moderate stable hyperglycemia, glucose intolerance, altered but significant glucose-stimulated insulin secretion, in vivo and in vitro. Tetrahydrocurcumin (THC) is one of the major colorless metabolite of curcumin. THC has been reported to exhibit the same physiological and pharmacological properties of curcumin. Curcumin is rapidly metabolized during absorption from the intestine, yielding THC, which has shown the strongest antioxidant activity among all curcuminoids. THC one of the active metabolites in curcumin on blood glucose and plasma insulin in streptozotocin induced diabetic rats. Different doses of THC (20, 40 and 80 mg\kg body weight) were orally administered to diabetic rats for 45 days, after which activities 6-weeks treatment with various doses of THC and curcumin on glucose levels were assayed

Evalution of anti Diabetic Activity for ethanolic extract Syzygium cumini leaf in Alloxan induced diabetic rat

2015 ◽  
Vol 1 (1) ◽  
pp. 27-31
Author(s):  
Maheswararao Chattu ◽  
Guptha Attyam
Keyword(s):  
Blood Glucose ◽  
Side Effects ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Diabetic Rats ◽  
Hypoglycemic Effect ◽  
Hypoglycemic Agents ◽  
Syzygium Cumini ◽  
Test Drug ◽  
Standard Drug

The available drugs for the treatment of diabetes, such as Insulin or Oral hypoglycemic agents have one or more side effects. So the search for newer anti-diabetic drugs with minimum or no side effects from herbal plants is a challenge as per world health organization (WHO) recommendations. Diabetes mellitus is a metabolic disorder of carbohydrate, protein and fat which are characterized by hyperglycemia, polyuria, polydipsia, and polyphagia which is deficient insulin production or ineffectiveness in insulin actions. The present study was aimed to evaluate the anti diabetic potency of syzygium cumini leaf on the blood glucose level in alloxan induced diabetic rats. Diabetic Wistar strain rats were treated with standard drug Glibenclamide and test drug syzygium cumini at 100mg, 200mg. The hypoglycemic effect was determined in the rats and the efficacy of the test drug was compared to the standard drug Glibenclamide. Syzygium cumini leaf was orally administered for 14 days in alloxan induced diabetic rats. At the end of the study duration blood glucose level and Biochemical parameters were statistically analyzed. Based on these results of the study this syzygium cumini produced a significant reduction in blood glucose levels when compared with diabetic control rats and hence the present research work proved that the syzygium cumini possess hypoglycemic effect.

scholarly journals Hypoglycemic Effect of Macrocyclic Binuclear Oxovanadium (IV) Complex on Streptozotocin-Induced Diabetic Rats

2004 ◽  
Vol 5 (2) ◽  
pp. 137-142 ◽  
Author(s):  
B. Ramachandran ◽  
D. Sathish Sekar ◽  
M. Kandaswamy ◽  
V. Narayanan ◽  
S. Subramanian
Keyword(s):  
Blood Glucose ◽  
Side Effects ◽  
Diabetic Rats ◽  
Spectral Studies ◽  
Vanadium Complexes ◽  
Vanadium Complex ◽  
Glucose Load ◽  
Oxovanadium Complex ◽  
Composition And Structure ◽  
Biochemical Studies

Though vanadium complexes mimic the action of insulin, owing to their toxicity, research is still in progress for a new vanadium complex with maximum efficacy at low concentration and without any side effects. A novel macrocyclic binuclear oxovanadium complex was synthesized, its composition and structure were confirmed by spectral studies and its efficacy was studied in streptozotocin-induced diabetic rats over a period of 30 days. The oral administration of the complex normalizes the blood glucose level in the diabetic rats and also maintains normoglycemia after a glucose load. The biochemical studies revealed that the complex is not toxic to the system. The nontoxic nature of this complex may be due to the presence of the vanadyl ions in an intact form. The study highlights the nontoxic and hypoglycemic effects of the new macrocyclic binuclear oxovanadium complex.

scholarly journals Antidiabetic Effect of Katakakhadiradi kashayam by Improving the Insulin Expression and Glucose Metabolising Enzyme

2021 ◽  
pp. 1-8
Author(s):  
Angelie Jessica Subbiah ◽  
M. Kavimani ◽  
Mukilan Ramadoss ◽  
Mudiganti Ram Krishna Rao ◽  
K. Prabhu
Keyword(s):  
Side Effects ◽  
Wistar Rats ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Β Cells ◽  
Metabolizing Enzymes ◽  
Antihyperglycemic Activity ◽  
Antidiabetic Effect ◽  
Pathological Conditions ◽  
Insulin Expression

Many plants provide a rich source of bioactive chemicals, which are free from undesirable side effects and possess powerful pharmacological actions. The present study was carried out to find the antidiabetic effect of   Katakakhadiradi kashayam (KKK) by improving the insulin expression and regulating properly the glucose metabolising enzymes. The diabetes was induced in combination with streptozotocin and nicotinamide injection to Wistar rats. Diabetic rats were treated with   Katakakhadiradi kashayam orally at doses of 100, 200 and 300 mg/kg/bw for 28 days, and the obtained results of parameters were compared with glibenclamide. The antidiabetic effect of Kashayam was measured by the expression of insulin by immunohistochemistry and restoring the normal clinical values of glucose metabolizing enzymes. The present study specified that hyperglycemia leads to pathological conditions in pancreatic tissue with decreased expression of insulin in β-cells whereas the   Katakakhadiradi kashayam normalised the production of insulin. The study found that the antihyperglycemic activity of   Katakakhadiradi kashayam L. is mainly due to their ability to restore the function of pancreatic tissues by causing an increase in insulin output and maintaining the glucose metabolising enzymes.

scholarly journals Improving the Reliability and Utility of Streptozotocin-Induced Rat Diabetic Model

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Yanlin Wang-Fischer ◽  
Tina Garyantes
Keyword(s):  
Side Effects ◽  
Sodium Channel ◽  
Pancreatic Islets ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Partial Recovery ◽  
Sprague Dawley ◽  
Area Reduction ◽  
Diabetic Model ◽  
Pancreatic Toxicity

The Streptozotocin- (STZ-) induced diabetic model is widely used; however, unexplained acute toxicity has given the model an unreliable reputation. To improve the reliability and utility of this model, we characterize the age dependence of STZ toxicity and introduce novel endpoints to assess diabetic complications and reveal possible mechanisms for diabetic development. Diabetes was induced by STZ injection into male, 6 to 23 weeks old, Sprague-Dawley rats. Their metabolic (glucose, lipids, and hormones), inflammatory (cytokines), histologic and behavioral endpoints were observed for 1.2 years. Analgesic compounds were assessed for efficacy treating neuropathy. Acute mortality, within a week of STZ injection (50–65 mg/kg i.v.), was inversely correlated to animal age. Only 3% of rats, age 6–11 weeks, died in the week following STZ injection, whereas 83% of rats 12 to 17 weeks old and 91% of rats 18 weeks or older died in the same week. Partial model recovery (normalized insulin, glucose and food/water intake) was observed starting at week 36; however, pain scores, kidney enlargement, and cataract formation continued to show progression consistent with the diabetic state. Unique noninvasive observational measurements, such as haircoat quality and diarrhea scores, served as useful endpoints for this model. The increased plasma cytokines (such as TNF-α, IL-4, and IL-6) and inflammatory cell infiltration into the pancreatic islets are strong evidence of inflammation in the STZ-induced diabetic model. Pancreatic tissue staining revealed total islet area reduction and confirmed STZ-specific pancreatic toxicity; however, the β-cell density per area in pancreatic islets and insulin levels statistically increased over time in the diabetic rats, suggesting a mechanism for partial recovery of diabetic symptoms. Voltage-gated sodium channel (NaV1.7 specific, peripherally restricted) blocker, CC4148, inhibited neuropathy without side effects as compared to a nonspecific sodium channel inhibitor, Mexiletine, or GABA analog, Pregabalin, which inhibited neuropathy with side effects.

Improvement of Glucose Homeostasis by Oral Vanadyl or Vanadate Treatment in Diabetic Rats is Accompanied by Negative Side Effects

1991 ◽  
Vol 68 (4) ◽  
pp. 249-253 ◽  
Author(s):  
José L. Domingo ◽  
Mercedes Gómez ◽  
Juan M. Llobet ◽  
Jacinto Corbella ◽  
Carl L. Keen
Keyword(s):  
Side Effects ◽  
Glucose Homeostasis ◽  
Diabetic Rats ◽  
Negative Side ◽  
Negative Side Effects

Sites of Insulin Action Using Ferritin Labeling

1971 ◽  
Vol 29 ◽  
pp. 540-541
Author(s):  
Burton B. Silver ◽  
Ronald S. Nelson
Keyword(s):  
Electron Microscopy ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Insulin Antibody ◽  
Fat Pad ◽  
Target Organs ◽  
Uranium Salts ◽  
Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

Gastric Ulcers Produced by Cisplatin

1981 ◽  
Vol 39 ◽  
pp. 582-583
Author(s):  
S.K. Aggarwal ◽  
J. San Antonio
Keyword(s):  
Electron Microscopy ◽  
Single Dose ◽  
Side Effects ◽  
Antitumor Agent ◽  
Gastric Ulcers ◽  
Enzyme Cytochemistry ◽  
Intestinal Toxicity ◽  
Ovarian Cancers ◽  
Inner Surface

Cisplatin (cis-dichlorodiammineplatinum(II)) a potent antitumor agent is now available for the treatment of testicular and ovarian cancers. It is however, not free from its serious side effects including nephrotoxicity, gastro intestinal toxicity, myelosuppression, and ototoxicity. Here we now report that the drug produces peculiar bloating of the stomach in rats and induces acute ulceration.Wistar-derived rats weighing 200-250 g were administered cisplatin(9 mg/kg) ip as a single dose in 0.15 M NaCl. After 3 days the animals were sacrificed by decapitation. The stomachs were removed, the contents analyzed for pepsin and acidity. The inner surface was examined with a dissecting microscope after a moderate stretching for ulcers. Affected areas were fixed and processed for routine electron microscopy and enzyme cytochemistry.The drug treated animals kept on food and water consistently showed bloating and lesions (Fig. 1) with a frequency of 6-70 ulcers in the rumen section of the stomachs.

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