scholarly journals Incorporating β-cyclodextrin into collagen scaffolds to sequester growth factors and modulate mesenchymal stem cell activity

2018 ◽  
Vol 76 ◽  
pp. 116-125 ◽  
Author(s):  
William K. Grier ◽  
Aleczandria S. Tiffany ◽  
Matthew D. Ramsey ◽  
Brendan A.C. Harley
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Mesenchymal Stem Cell ◽  
Cell Activity ◽  
Collagen Scaffolds ◽  
Stem Cell Activity

scholarly journals Hair-follicle mesenchymal stem-cell activity during homeostasis and wound healing

2021 ◽  
Author(s):  
Emil Aamar ◽  
Efrat Avigad Laron ◽  
Wisal Asaad ◽  
Sarina Harshuk-Shabso ◽  
David Enshell-Seijffers
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Cell Activity ◽  
Stem Cell Activity

Fibroblast Growth Factors Regulate Stem Cell Functioning In Vitro and in Vivo.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1705-1705
Author(s):  
Joyce S.G Yeoh ◽  
Ronald van Os ◽  
Ellen Weersing ◽  
Bert Dontje ◽  
Edo Vellenga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Cultured Cells ◽  
Cell Activity ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Stem Cell Activity

Abstract Fibroblast Growth Factors (FGF) are a large family of signaling molecules widely involved in tissue development, maintenance and repair. Little is known about the role of FGF/FGF-receptor signaling in the regulation of adult hematopoietic stem cells (HSC). In this study, we assessed the potential of exogenously added FGF-1/2, or retrovirally overexpressed FGF-1 to preserve HSC function in vitro and in vivo. First, we demonstrate that in vitro culture of unfractionated mouse bone marrow cells, in serum-free medium, supplemented with FGF-1 or FGF-2 or FGF-1 + 2 resulted in the robust generation of long-term repopulating (LTR) HSCs. Cultures were maintained for 12 weeks and during that time in vivo competitive reconstitution assays were performed. Stem cell activity was detectable at 3, 5, and 8 weeks after initiation of culture, but lost after 12 weeks. However, whereas 3 and 5 week cultured cells provided radioprotection in non-competitive assays, animals transplanted with 8 or 12 week cultured cells succumbed due to bone marrow failure. So far, we have been unable to expand single, highly purified Lin−Sca-1+c-Kit+ using FGF-1 + 2. Consequently, we speculated that essential intermediate cell populations or signals are required for FGF-induced stem cell conservation. To test this we cultured highly purified CD45.1 Lin−Sca-1+c-Kit+ cells in a co-culture with CD45.2 unfractionated BM. Co-cultured cells were transplanted after 5 weeks in lethally irradiated recipients, and CD45.1 chimerism levels were assessed. High levels of CD45.1 chimerism confirmed that Lin−Sca-1+c-Kit+ cells require an accessory signal in addition to FGF to induced stem cell activity in vitro. We subsequently tested stem cell potential of cells cultured in FGF-1 + 2 for 5 weeks, with the addition of SCF + IL-11 + Flt3L for the last 2, 4 or 7 days. Cell numbers increased with increasing time of growth factor presence. However, only when growth factors were present for 2 days engraftment of cultured cells in a competitive repopulation assay was increased 3.5-fold. Finally, we show by immunohistochemistry that ~10% of freshly isolated Lin−Sca-1+c-Kit+ expresses high levels of FGF-1. Retroviral overexpression of FGF-1 in stem cells resulted in increased growth potential and sustained clonogenic activity in vitro. Upon transplantation of transduced stem cells, FGF-1 overexpression resulted in increased white blood cell counts 4 weeks post-transplant compared to control animals. Most notable was a marked granulocytosis in FGF-1 overexpressing recipients Our results reveal FGF as an important regulator of HSC signaling and homeostasis. Importantly, in the presence of FGF stem cells can be maintained in vitro for 2 months. These findings open novel avenues for in vitro manipulation of stem cells for future clinical therapies.

Regulation of Mesenchymal Stem Cell Activity by Endothelial Cells

2011 ◽  
Vol 20 (3) ◽  
pp. 391-403 ◽  
Author(s):  
Fatima A. Saleh ◽  
Muna Whyte ◽  
Peter Ashton ◽  
Paul G. Genever
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Activity ◽  
Stem Cell Activity

scholarly journals Effects of Poor Maternal Nutrition during Gestation on Bone Development and Mesenchymal Stem Cell Activity in Offspring

PLoS ONE ◽  
2016 ◽  
Vol 11 (12) ◽  
pp. e0168382 ◽  
Author(s):  
Sambhu M. Pillai ◽  
Nicole H. Sereda ◽  
Maria L. Hoffman ◽  
Ellen V. Valley ◽  
Thomas D. Crenshaw ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Maternal Nutrition ◽  
Bone Development ◽  
Cell Activity ◽  
Stem Cell Activity

scholarly journals Impact of obesity on bone integrity and mesenchymal stem cell activity in Ossabaw swine

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Brynn Shea Seabolt ◽  
J W Perfield ◽  
R S Rector ◽  
C H Stahl
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Activity ◽  
Stem Cell Activity
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