Fe3O4/BSA particles induce osteogenic differentiation of mesenchymal stem cells under static magnetic field

2016 ◽  
Vol 46 ◽  
pp. 141-150 ◽  
Author(s):  
Pengfei Jiang ◽  
Yixian Zhang ◽  
Chaonan Zhu ◽  
Wenjing Zhang ◽  
Zhengwei Mao ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Static Magnetic Field

Cellulose Acetate/Magnetic Graphene Nanofiber in Enhanced Human Mesenchymal Stem Cells Osteogenic Differentiation Under Alternative Current Magnetic Field

SPIN ◽  
2019 ◽  
Vol 09 (02) ◽  
pp. 1940011 ◽  
Author(s):  
Shadie Hatamie ◽  
Farzaneh Mohamadyar-Toupkanlou ◽  
Samaneh Mirzaei ◽  
Mohammad Mahdi Ahadian ◽  
Simzar Hosseinzadeh ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Osteogenic Differentiation ◽  
Cellulose Acetate ◽  
Magnetic Graphene ◽  
Nanocomposite Scaffold ◽  
Alternative Current ◽  
Current Magnetic Field

The three-dimensional (3D) nano scaffold of the cellulose acetate (CA) containing graphene/cobalt nanocomposite (0.1[Formula: see text]wt.%) was fabricated via electrospinning technique, and its impact on bone regeneration was investigated. Through this aim, bone marrow mesenchymal stem cells are cultured on the CA, and graphene/cobalt (rGO/Co)/CA nanocomposite scaffold surfaces and the samples are treated under low frequency alternative magnetic field (75[Formula: see text]Hz). The scaffolds are characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermal studies (TG/DSC). The proliferation behavior of stem cells on CA, and rGO/Co/CA nano scaffolds are studied by MTT assay, show their biocompability after 14 days of cell seeding. The 6-diamidino-2-phenylindole (DAPI) staining is used to confirm the morality of stem cell for duration of seven days. The nanocomposite scaffold is enhanced for extremely higher proliferation compared to the bare CA scaffold. The acceleration on osteogenic differentiation on the bone mesenchymal stem cell is enhanced within 48[Formula: see text]h when rGO/Co/CA scaffold is placed under alternative current magnetic field (ACMF). Furthermore, the acceleration of the stem cells differentiation for the rGO/Co/CA scaffold under ACMF corresponds to the induced scaffold surface roughness caused by graphene sheets, the metallic behavior of graphene and the responding of the nanocomposite magnetic parts (i.e., cobalt nanoparticles) while applying 75[Formula: see text]Hz frequency. Using reverse transcription polymerase chain reaction (RT-PCR) analysis, the superior effect of ACMF on scaffold contain magnetic graphene nanocomposite is confirmed to produce bone related genes within 14 days.

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