Low, but not too low, oxygen tension and macromolecular crowding accelerate extracellular matrix deposition in human dermal fibroblast culture

2016 ◽  
Vol 44 ◽  
pp. 221-231 ◽  
Author(s):  
Abhigyan Satyam ◽  
Pramod Kumar ◽  
Daniela Cigognini ◽  
Abhay Pandit ◽  
Dimitrios I. Zeugolis
Keyword(s):  
Extracellular Matrix ◽  
Oxygen Tension ◽  
Dermal Fibroblast ◽  
Macromolecular Crowding ◽  
Human Dermal Fibroblast ◽  
Fibroblast Culture ◽  
Low Oxygen ◽  
Matrix Deposition ◽  
Low Oxygen Tension ◽  
Extracellular Matrix Deposition

The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents

2020 ◽  
Vol 12 (2) ◽  
pp. 025018 ◽  
Author(s):  
Dimitrios Tsiapalis ◽  
Andrea De Pieri ◽  
Kyriakos Spanoudes ◽  
Ignacio Sallent ◽  
Stephen Kearns ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Synergistic Effect ◽  
Oxygen Tension ◽  
Macromolecular Crowding ◽  
Low Oxygen ◽  
Low Oxygen Tension

Glucosamine sulphate does not increase extracellular matrix production at low oxygen tension

2009 ◽  
Vol 337 (1) ◽  
pp. 103-111 ◽  
Author(s):  
Cheng-Juan Qu ◽  
Teemu Pöytäkangas ◽  
Marjo Jauhiainen ◽  
Seppo Auriola ◽  
Mikko J. Lammi
Keyword(s):  
Extracellular Matrix ◽  
Oxygen Tension ◽  
Low Oxygen ◽  
Low Oxygen Tension ◽  
Extracellular Matrix Production ◽  
Glucosamine Sulphate ◽  
Matrix Production

Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding

Biomaterials ◽  
2015 ◽  
Vol 73 ◽  
pp. 60-69 ◽  
Author(s):  
Marina C. Prewitz ◽  
Aline Stißel ◽  
Jens Friedrichs ◽  
Nicole Träber ◽  
Steffen Vogler ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Macromolecular Crowding ◽  
Bone Marrow Stroma ◽  
Matrix Deposition ◽  
Marrow Stroma ◽  
Extracellular Matrix Deposition

Low Oxygen Tension Decreases Receptor Binding of Peptide Growth Factors in Dermal Fibroblast Cultures

1994 ◽  
Vol 213 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Vincent Falanga ◽  
Hajime Takagi ◽  
Patricia I. Ceballos ◽  
Jeffrey B. Pardes
Keyword(s):  
Growth Factors ◽  
Oxygen Tension ◽  
Receptor Binding ◽  
Dermal Fibroblast ◽  
Low Oxygen ◽  
Fibroblast Cultures ◽  
Peptide Growth Factors ◽  
Low Oxygen Tension

scholarly journals Adapting the Scar-in-a-Jar to Skin Fibrosis and Screening Traditional and Contemporary Anti-Fibrotic Therapies

2021 ◽  
Vol 9 ◽  
Author(s):  
João Q. Coentro ◽  
Ulrike May ◽  
Stuart Prince ◽  
John Zwaagstra ◽  
Olli Ritvos ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Histone Deacetylases ◽  
Transforming Growth Factor ◽  
Dermal Fibroblast ◽  
Trichostatin A ◽  
Macromolecular Crowding ◽  
Skin Fibrosis ◽  
Type I ◽  
Matrix Deposition

Skin fibrosis still constitutes an unmet clinical need. Although pharmacological strategies are at the forefront of scientific and technological research and innovation, their clinical translation is hindered by the poor predictive capacity of the currently available in vitro fibrosis models. Indeed, customarily utilised in vitro scarring models are conducted in a low extracellular matrix milieu, which constitutes an oxymoron for the in-hand pathophysiology. Herein, we coupled macromolecular crowding (enhances and accelerates extracellular matrix deposition) with transforming growth factor β1 (TGFβ1; induces trans-differentiation of fibroblasts to myofibroblasts) in human dermal fibroblast cultures to develop a skin fibrosis in vitro model and to screen a range of anti-fibrotic families (corticosteroids, inhibitors of histone deacetylases, inhibitors of collagen crosslinking, inhibitors of TGFβ1 and pleiotropic inhibitors of fibrotic activation). Data obtained demonstrated that macromolecular crowding combined with TGFβ1 significantly enhanced collagen deposition and myofibroblast transformation. Among the anti-fibrotic compounds assessed, trichostatin A (inhibitors of histone deacetylases); serelaxin and pirfenidone (pleiotropic inhibitors of fibrotic activation); and soluble TGFβ receptor trap (inhibitor of TGFβ signalling) resulted in the highest decrease of collagen type I deposition (even higher than triamcinolone acetonide, the gold standard in clinical practice). This study further advocates the potential of macromolecular crowding in the development of in vitro pathophysiology models.

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