Xenogeneic cardiac extracellular matrix scaffolds with or without seeded mesenchymal stem cells exhibit distinct in vivo immunosuppressive and regenerative properties

2016 ◽  
Vol 45 ◽  
pp. 155-168 ◽  
Author(s):  
Angela Papalamprou ◽  
Chia Wei Chang ◽  
Natalia Vapniarsky ◽  
Alycia Clark ◽  
Naomi Walker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Extracellular Matrix Scaffolds

scholarly journals Tumor Immunotherapy Using Gene-Modified Human Mesenchymal Stem Cells Loaded into Synthetic Extracellular Matrix Scaffolds

Stem Cells ◽  
2009 ◽  
Vol 27 (3) ◽  
pp. 753-760 ◽  
Author(s):  
Marta Compte ◽  
Ángel M. Cuesta ◽  
David Sánchez-Martín ◽  
Vanesa Alonso-Camino ◽  
José Luís Vicario ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Tumor Immunotherapy ◽  
Extracellular Matrix Scaffolds

scholarly journals Extracellular matrix modulates the biological effects of melatonin in mesenchymal stem cells

2014 ◽  
Vol 223 (2) ◽  
pp. 167-180 ◽  
Author(s):  
Fan He ◽  
Xiaozhen Liu ◽  
Ke Xiong ◽  
Sijin Chen ◽  
Long Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Biological Effects ◽  
Matrix Composition ◽  
Calcium Deposition ◽  
Specific Gene ◽  
Skeletal Tissue ◽  
Underlying Mechanisms

Both self-renewal and lineage-specific differentiation of mesenchymal stem cells (MSCs) are triggered by theirin vivomicroenvironment including the extracellular matrix (ECM) and secreted hormones. The ECM may modulate the physiological functions of hormones by providing binding sites and by regulating downstream signaling pathways. Thus, the purpose of this study was to evaluate the degree of adsorption of melatonin to a natural cell-deposited ECM and the effects of this interaction on the biological functions of melatonin in human bone marrow-derived MSCs (BM-MSCs). The fibrillar microstructure, matrix composition, and melatonin-binding affinity of decellularized ECM were characterized. The cell-deposited ECM improved melatonin-mediated cell proliferation by 31.4%, attenuated accumulation of intracellular reactive oxygen species accumulation, and increased superoxide dismutase (SOD) mRNA and protein expression. Interaction with ECM significantly enhanced the osteogenic effects of melatonin on BM-MSCs by increasing calcium deposition by 30.5%, up-regulating osteoblast-specific gene expression and down-regulating matrix metalloproteinase (MMP) expression. The underlying mechanisms of these changes in expression may involve intracellular antioxidant enzymes, because osteoblast-specific genes were down-regulated, whereas MMP expression was up-regulated, in the presence of SOD-specific inhibitors. Collectively, our findings indicate the importance of native ECM in modulating the osteoinductive and antioxidant effects of melatonin and provide a novel platform for studying the biological actions of growth factors or hormones in a physiologically relevant microenvironment. Moreover, a better understanding of the enhancement of MSC growth and osteogenic differentiation resulting from the combination of ECM and melatonin could improve the design of graft substitutes for skeletal tissue engineering.

scholarly journals Effects of Extracellular Matrix Density and Mesenchymal Stem Cells on Neovascularization In Vivo

2011 ◽  
Vol 17 (7-8) ◽  
pp. 905-914 ◽  
Author(s):  
Ekaterina Kniazeva ◽  
Suraj Kachgal ◽  
Andrew J. Putnam
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Matrix Density

Composite Silk-Extracellular Matrix Scaffolds for Enhanced Chondrogenesis of Mesenchymal Stem Cells

2018 ◽  
Vol 24 (11) ◽  
pp. 645-658 ◽  
Author(s):  
Xiao Zhang ◽  
Chenjun Zhai ◽  
Hao Fei ◽  
Yang Liu ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Extracellular Matrix Scaffolds
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