scholarly journals Influence of select extracellular matrix proteins on mesenchymal stem cell osteogenic commitment in three-dimensional contexts

2012 ◽  
Vol 8 (12) ◽  
pp. 4397-4404 ◽  
Author(s):  
Silvia Becerra-Bayona ◽  
Viviana Guiza-Arguello ◽  
Xin Qu ◽  
Dany J. Munoz-Pinto ◽  
Mariah S. Hahn
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins

scholarly journals Modulation of mesenchymal stem cell genotype and phenotype by extracellular matrix proteins

2016 ◽  
Vol 57 (6) ◽  
pp. 443-453 ◽  
Author(s):  
Lucy E. Clements ◽  
Elaine R. Garvican ◽  
Jayesh Dudhia ◽  
Roger K. W. Smith
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins

scholarly journals Bio-inks for 3D bioprinting: recent advances and future prospects

2017 ◽  
Vol 8 (31) ◽  
pp. 4451-4471 ◽  
Author(s):  
Ilze Donderwinkel ◽  
Jan C. M. van Hest ◽  
Neil R. Cameron
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Cell Aggregates ◽  
3D Bioprinting ◽  
Future Prospects ◽  
Printing Inks ◽  
Polymer Bead ◽  
Polymeric Hydrogels

In the last decade, interest in the field of three-dimensional (3D) bioprinting has increased enormously. This review describes all the currently used bio-printing inks, including polymeric hydrogels, polymer bead microcarriers, cell aggregates and extracellular matrix proteins.

scholarly journals Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

2019 ◽  
Vol 20 (16) ◽  
pp. 3970 ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Susumu Horikoshi ◽  
Tomoya Ogawa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Host Cells ◽  
Dependent Manner ◽  
Serum Free ◽  
Serum Free Conditions ◽  
Free Serum

Three-dimensional clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. We demonstrated previously that C-MSCs can be transplanted into bone defect regions with no artificial scaffold to induce bone regeneration. To apply C-MSCs in a clinical setting as a reliable bone regenerative therapy, the present study aimed to generate C-MSCs in xeno-free/serum-free conditions that can exert successful bone regenerative properties and to monitor interactions between grafted cells and host cells during bone healing processes. Human bone marrow-derived MSCs were cultured in xeno-free/serum-free medium. To obtain C-MSCs, confluent cells that had formed on the cellular sheet were scratched using a micropipette tip and then torn off. The sheet was rolled to make a round clump of cells. Then, C-MSCs were transplanted into an immunodeficient mouse calvarial defect model. Transplantation of C-MSCs induced bone regeneration in a time-dependent manner. Immunofluorescence staining showed that both donor human cells and host mice cells contributed to bone reconstruction. Decellularized C-MSCs implantation failed to induce bone regeneration, even though the host mice cells can infiltrate into the defect area. These findings suggested that C-MSCs generated in xeno-free/serum-free conditions can induce bone regeneration via direct and indirect osteogenesis.

Identification of the Critical Extracellular Matrix Proteins that Promote Human Embryonic Stem Cell Assembly

2009 ◽  
Vol 18 (6) ◽  
pp. 919-928 ◽  
Author(s):  
Denis Evseenko ◽  
Katja Schenke-Layland ◽  
Gautam Dravid ◽  
Yuhua Zhu ◽  
Qian-Lin Hao ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Cell Assembly

Neural stem cell neural differentiation in 3D extracellular matrix and endoplasmic reticulum stress microenvironment

RSC Advances ◽  
2016 ◽  
Vol 6 (41) ◽  
pp. 34959-34969 ◽  
Author(s):  
Yan-Qiu Liu ◽  
Li-Bin Zhan ◽  
Ting-Ting Bi ◽  
Li-Na Liang ◽  
Xiao-Xin Sun ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endoplasmic Reticulum ◽  
Stem Cell ◽  
Endoplasmic Reticulum Stress ◽  
Neural Stem Cell ◽  
Neural Differentiation ◽  
Stress Condition ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins

Neural stem cell neural differentiation was protected by nanomatrix and extracellular matrix proteins under the endoplasmic reticulum stress condition.

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