Strontium-containing mesoporous bioactive glass scaffolds with improved osteogenic/cementogenic differentiation of periodontal ligament cells for periodontal tissue engineering

2012 ◽  
Vol 8 (10) ◽  
pp. 3805-3815 ◽  
Author(s):  
Chengtie Wu ◽  
Yinghong Zhou ◽  
Chucheng Lin ◽  
Jiang Chang ◽  
Yin Xiao
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Mesoporous Bioactive Glass

Physiological features of periodontal regeneration and approaches for periodontal tissue engineering utilizing periodontal ligament cells

2007 ◽  
Vol 103 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Bruno Braga Benatti ◽  
Karina Gonzales Silvério ◽  
Márcio Zaffalon Casati ◽  
Enílson Antônio Sallum ◽  
Francisco Humberto Nociti
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue

Periodontal Tissue Engineering with a Multiphasic Construct and Cell Sheets

2019 ◽  
Vol 98 (6) ◽  
pp. 673-681 ◽  
Author(s):  
C. Vaquette ◽  
S. Saifzadeh ◽  
A. Farag ◽  
D.W. Hutmacher ◽  
S. Ivanovski
Keyword(s):  
Tissue Engineering ◽  
Periodontal Ligament ◽  
Cell Sheet ◽  
Periodontal Regeneration ◽  
Surrounding Tissue ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Micro Computed Tomography ◽  
Cell Sheets ◽  
Tissue Integration

This study reports on scaffold-based periodontal tissue engineering in a large preclinical animal model. A biphasic scaffold consisting of bone and periodontal ligament compartments manufactured by melt and solution electrospinning, respectively, was used for the delivery of in vitro matured cell sheets from 3 sources: gingival cells (GCs), bone marrow–derived mesenchymal stromal cells (Bm-MSCs), and periodontal ligament cells (PDLCs). The construct featured a 3-dimensional fibrous bone compartment with macroscopic pore size, while the periodontal compartment consisted of a flexible porous membrane for cell sheet delivery. The regenerative performance of the constructs was radiographically and histologically assessed in surgically created periodontal defects in sheep following 5 and 10 wk of healing. Histologic observation demonstrated that the constructs maintained their shape and volume throughout the entirety of the in vivo study and were well integrated with the surrounding tissue. There was also excellent tissue integration between the bone and periodontal ligament compartments as well as the tooth root interface, enabling the attachment of periodontal ligament fibers into newly formed cementum and bone. Bone coverage along the root surface increased between weeks 5 and 10 in the Bm-MSC and PDLC groups. At week 10, the micro–computed tomography results showed that the PDLC group had greater bone fill as compared with the empty scaffold, while the GC group had less bone than the 3 other groups (control, Bm-MSC, and PDLC). Periodontal regeneration, as measured by histologically verified new bone and cementum formation with obliquely inserted periodontal ligament fibers, increased between 5 and 10 wk for the empty, Bm-MSC, and PDLC groups, while the GC group was inferior to the Bm-MSC and PDLC groups at 10 wk. This study demonstrates that periodontal regeneration can be achieved via the utilization of a multiphasic construct, with Bm-MSCs and PDLCs obtaining superior results as compared with GC-derived cell sheets.

scholarly journals Biomaterial-Based Approaches for Regeneration of Periodontal Ligament and Cementum Using 3D Platforms

2019 ◽  
Vol 20 (18) ◽  
pp. 4364 ◽  
Author(s):  
Chan Ho Park
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
State Of The Art ◽  
Tooth Root ◽  
Tissue Formation ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Root Surfaces ◽  
Periodontal Ligaments

Currently, various tissue engineering strategies have been developed for multiple tissue regeneration and integrative structure formations as well as single tissue formation in musculoskeletal complexes. In particular, the regeneration of periodontal tissues or tooth-supportive structures is still challenging to spatiotemporally compartmentalize PCL (poly-ε-caprolactone)-cementum constructs with micron-scaled interfaces, integrative tissue (or cementum) formations with optimal dimensions along the tooth-root surfaces, and specific orientations of engineered periodontal ligaments (PDLs). Here, we discuss current advanced approaches to spatiotemporally control PDL orientations with specific angulations and to regenerate cementum layers on the tooth-root surfaces with Sharpey’s fiber anchorages for state-of-the-art periodontal tissue engineering.

scholarly journals Effects of methanandamide on human periodontal ligament cells

2019 ◽  
Author(s):  
Fengqiu Zhang ◽  
Burcu Özdemir ◽  
Phuong Quynh Nguyen ◽  
Oleh Andrukhov ◽  
Xiaohui Rausch-Fan
Keyword(s):  
Periodontal Ligament ◽  
Cannabinoid Receptors ◽  
Endocannabinoid System ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Inflammatory Conditions ◽  
Human Periodontal Ligament Cells ◽  
Chemotactic Protein ◽  
Improved Stability ◽  
Porphyromonas Gingivalis Lipopolysaccharide

Abstract Background Endocannabinoid system is involved in the regulation of periodontal tissue homeostasis. Synthetic cannabinoid methanandamide (Meth-AEA) has an improved stability and affinity to cannabinoid receptors compared to its endogenous analogue anandamide. In the present study, we investigated the effect of methanandamide on the production of pro-inflammatory mediators in primary human periodontal ligament cells (hPdLCs). Methods hPdLCs were treated with Meth-AEA for 24 h and resulting production of interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1 was measured under normal condition as well as under inflammatory conditions mimicked by the presence of Porphyromonas gingivalis lipopolysaccharide (LPS). Additionally, the effect of Meth-AEA on the proliferation/viability of hPdLCs was measured by MTT method. Results Methanandamide at concentration of 10 µM significantly inhibited P. gingivalis LPS induced production of IL-6, IL-8, and MCP-1. Basal production of IL-6 and IL-8 was slightly enhanced by 10 µM Meth-AEA. No effect of Meth-AEA on the basal production of MCP-1 was observed. Proliferation/viability of hPdLCs was not affected by Meth-AEA in concentrations up to 10 µM and was significantly inhibited by 30 µM Meth-AEA. Conclusion Our study supports the influence of cannabinoid system on the inflammatory processes in periodontal tissue and its potential involvement in the progression of periodontal disease.

scholarly journals Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

2020 ◽  
Vol 5 (5) ◽  
pp. 28-34
Author(s):  
Olena J. Kordiyak ◽  
Keyword(s):  
Tissue Engineering ◽  
Animal Models ◽  
Periodontal Disease ◽  
Periodontal Ligament ◽  
Periodontal Regeneration ◽  
Sufficient Evidence ◽  
Computer Assisted ◽  
Periodontal Tissue ◽  
Periodontal Tissues ◽  
Computed Tomography Scanning

Chronic periodontitis is a common dental disease, resulting in destruction of gingival tissue, periodontal ligament, cementum, alveolar bone and, consequently- teeth loss in the adult population. Experimental animal models have enabled the study of periodontal disease pathogenesis and are used to test new therapeutic approaches for treating the disease The purpose of this review study was to draw the evidence from animal models, required for future assessment of destructional and regenerative processes in periodontal tissues. Material and methods: a rat experimental periodontitis models of ligature, streptozotocin, and immune complexes induced periodontitis, periodontal defect, altered functional loading, stress exposures and surgically created chronic acid reflux esophagitis models. Histomorphomorphological/-metrical, immunohisto (-cyto)chemical and histopathological analysis, micro-computed tomography, scanning and transmission electron microscopy, polarizing light and confocal microscopy, spectrophotometry, radiographic and biomechanical analysis, descriptive histology and computer-assisted image analysis. Results and discussion. Scaling and root planing may not always be effective in preventing periodontal disease progression, and, moreover, with currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved. However, in 70.5% of the results of experimental studies reported, irrespective of the defect type and animal model used, beneficial outcome for periodontal regeneration after periodontal ligament stem cell implantation, including new bone, new cementum and new connective tissue formation, was recorded. Therefore, platelet-rich fibrin combined with rat periodontal ligament stem cells provides a useful instrument for periodontal tissue engineering. Conclusion. There is sufficient evidence from preclinical animal studies suggesting that periodontal tissue engineering would provide a valuable tool for periodontal regeneration. Further elaboration of the developed in preclinical studies experimental techniques should justify progress to clinical studies and subsequent medical application

Application of eGFP to label human periodontal ligament stem cells in periodontal tissue engineering

2012 ◽  
Vol 57 (9) ◽  
pp. 1241-1250 ◽  
Author(s):  
Yong Wen ◽  
Jing Lan ◽  
Haiyun Huang ◽  
Meijiao Yu ◽  
Jun Cui ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Periodontal Ligament ◽  
Periodontal Tissue ◽  
Periodontal Ligament Stem Cells

Effect of Incorporation of Nanoscale Bioactive Glass and Hydroxyapatite in PCL/Chitosan Nanofibers for Bone and Periodontal Tissue Engineering

2013 ◽  
Vol 9 (3) ◽  
pp. 430-440 ◽  
Author(s):  
K. T. Shalumon ◽  
S. Sowmya ◽  
D. Sathish ◽  
K. P. Chennazhi ◽  
Shantikumar V. Nair ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Periodontal Tissue
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