Poly(lactic–co-glycolic acid): Carbon nanofiber composites for myocardial tissue engineering applications

2011 ◽  
Vol 7 (8) ◽  
pp. 3101-3112 ◽  
Author(s):  
David A. Stout ◽  
Bikramjit Basu ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
Carbon Nanofiber ◽  
Myocardial Tissue ◽  
Engineering Applications ◽  
Myocardial Tissue Engineering

Poly Lactic-Co-Glycolic Acid Carbon Nanofiber Composite for Enhancing Cardiomyocyte Function

2011 ◽  
Vol 1316 ◽  
Author(s):  
David A. Stout ◽  
Jennie Yoo ◽  
Thomas J. Webster
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanofibers ◽  
Glycolic Acid ◽  
Carbon Nanofiber ◽  
Myocardial Tissue ◽  
Engineering Applications ◽  
Nanofiber Composite ◽  
Human Cardiomyocytes ◽  
Myocardial Tissue Engineering

ABSTRACTThe objective of the present in vitro research was to determine cardiomyocyte function on poly lactic-co-glycolic acid (50:50 (PLA:PGA); PLGA) with greater amounts of carbon nanofibers (CNFs) and variations in CNF size, for myocardial tissue engineering applications. The addition of CNFs would increase conductivity and strength of pure PLGA. For this reason, different PLGA: CNF ratios (100:0, 75:25, 50:50, 25:75, 0:100 wt.%) were created and conductivity and cytocompatibility properties with human cardiomyocytes were determined. Results showed that PLGA:CNF materials were conductive and that conductivity increased with greater amounts of PLGA added, from 0 S.m-1 for 100:0 wt.% (pure PLGA) to 5.5x10-3 S.m-1 for 0:100 wt.% (pure CNFs) material. Furthermore, results indicated that cardiomyocyte density increased with greater amounts of CNFs of 200nm in diameter in PLGA (up to 25:75 wt.% , PLGA:CNFs). This study, thus, provided an alternative conductive scaffold using nanotechnology which should be further explored for cardiovascular applications.

Improved Cardiomyocyte Functions of Carbon Nanofiber Cardiac Patches

2012 ◽  
Vol 1417 ◽  
Author(s):  
David A. Stout ◽  
Emilia Raimondo ◽  
Thomas J. Webster
Keyword(s):  
Electrical Stimulation ◽  
Glycolic Acid ◽  
Troponin I ◽  
Carbon Nanofiber ◽  
Myocardial Tissue ◽  
Human Cardiomyocytes ◽  
Cell Functions ◽  
Stimulation System ◽  
Myocardial Tissue Engineering

ABSTRACTThe objective of the present in vitro research was to determine cardiomyocyte functions on poly-lactic-co-glycolic acid (50:50 (PLA:PGA); PLGA) with greater amounts of carbon nanofibers (CNFs) using an in vitro electrical stimulation system for myocardial tissue engineering applications. The addition of CNFs can increase the conductivity and strength of pure PLGA. For this reason, different PLGA: CNF ratios (100:0, 75:25, 50:50, 25:75, 0:100 wt%) were created where conductivity and cytocompatibility properties under electrical stimulation with human cardiomyocytes were determined. Results showed that PLGA:CNF materials were conductive and that conductivity increased with greater amounts of PLGA added, from 0 S.m-1 for 100:0 wt% (pure PLGA) to 6.5x10-3 S.m-1 for 0:100 wt% (pure CNFs) materials. Furthermore, results indicated that cardiomyocyte cell density increased with continuous electrical stimulation (rectangular, 2 nm, 5 V/cm, 1 Hz) after 1, 3, and 5 days as well as a slight increase in Troponin I excretion compared to non-electrically stimulated normal cardiomyocyte cell functions. This study, thus, provides an alternative conductive scaffold using nanotechnology which should be further explored for numerous cardiovascular applications.

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