WITHDRAWN: Calcium phosphate nanoparticles as gene therapy vectors show the advantage for short-term hard tissue formation when compared with adenovirus

2011 ◽  
Author(s):  
Xuechao Yang ◽  
X. Frank Walboomers ◽  
Zhuan Bian ◽  
John A. Jansen ◽  
Mingwen Fan
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Short Term ◽  
Gene Therapy Vectors ◽  
Calcium Phosphate Nanoparticles

scholarly journals Glutamine-chitosan modified calcium phosphate nanoparticles for efficient siRNA delivery and osteogenic differentiation

2015 ◽  
Vol 3 (31) ◽  
pp. 6448-6455 ◽  
Author(s):  
Bogyu Choi ◽  
Zhong-Kai Cui ◽  
Soyon Kim ◽  
Jiabing Fan ◽  
Benjamin M. Wu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Gene Transfection ◽  
Sirna Delivery ◽  
Calcium Phosphate Nanoparticles

CaP nanoparticles coated with highly cationic, glutamine-conjugated oligochitosan (Gln-OChi) are developed for siRNA delivery to significantly enhance gene transfection and knockdown efficiency with minimal cytotoxicity. This new nanocarrier can potentially be used for gene therapy.

scholarly journals Functionalized Calcium Phosphate Nanoparticles for Biomedical Application

2010 ◽  
Vol 441 ◽  
pp. 299-305 ◽  
Author(s):  
Matthias Epple ◽  
Anna Kovtun
Keyword(s):  
Calcium Phosphate ◽  
Nucleic Acids ◽  
Intracellular Calcium ◽  
Calcium Level ◽  
Biomedical Application ◽  
Hard Tissue ◽  
Chemical Similarity ◽  
Monodisperse Nanoparticles ◽  
Intracellular Calcium Level ◽  
Calcium Phosphate Nanoparticles

Calcium phosphate is a natural biomineral and therefore possesses an excellent biocompatibility due to its chemical similarity to human hard tissue (bone and teeth). Calcium phosphate nanoparticles can be precipitated under controlled conditions and used as carrier in biological systems, e.g. to transfer nucleic acids or drugs. Such nanoparticles can also be suitably functionalized with fluorescing dyes, polymeric agents, pro-drugs or activators. The small monodisperse nanoparticles only mildly influence the intracellular calcium level and therefore are not toxic for cells.

PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES AS NON-VIRAL VECTORS FOR GENE DELIVERY SYSTEM

2017 ◽  
Vol 29 (04) ◽  
pp. 1750027 ◽  
Author(s):  
Ko-Chung Yen ◽  
I-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Fluorescent Protein ◽  
Molar Ratio ◽  
Gene Delivery System ◽  
Calcium Phosphate Nanoparticles

A major aim of gene therapy is the efficient and specific delivery of therapeutic gene into the desired target tissues. Development of reliable vectors is a major challenge in gene therapy. The aim of this study is to develop calcium phosphate nanoparticles as novel non-viral vectors for the gene delivery system. Calcium phosphate nanoparticles were prepared by water-in-oil microemulsion method with a water to surfactant molar ratio, Wo [Formula: see text] 2–10. This paper studies the design and synthesis of ultra-low size, highly monodispersed DNA doped calcium phosphate nanoparticles of size around 100[Formula: see text]nm in diameter. The structure of DNA-calcium phosphate nanocomplex observed by TEM was displayed as a shell-like structure. This study used pEGFP as a reporter gene. The encapsulating efficiency to encapsulate DNA inside the nanoparticles was greater than 80%. In the MTT test, both calcium phosphate nanoparticles and DNA-calcium phosphate nanocomplex have no negative effect for 293T cells. By gel electrophoresis of free and entrapped pEGFP DNA, the DNA encapsulated inside the nanoparticles was protected from the external DNaseI environment. In vitro transfection studies in 293T cell-line, the DNA-calcium phosphate nanocomplex could be used safely to transfer the encapsulated DNA into the 293T cells and expression green fluorescent protein. The characteristic of DNA-calcium phosphate nanocomplex to deliver DNA belongs to slow release. The property of DNA-calcium phosphate nanocomplex was fit in the requirement of non-viral vectors for the gene delivery system.

scholarly journals Calcium Phosphate Nanoparticles-Based Systems for RNAi Delivery: Applications in Bone Tissue Regeneration

Nanomaterials ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 146 ◽  
Author(s):  
Tanya J. Levingstone ◽  
Simona Herbaj ◽  
John Redmond ◽  
Helen O. McCarthy ◽  
Nicholas J. Dunne
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Viral Vectors ◽  
Bone Repair ◽  
Transfection Efficiency ◽  
Bone Tissue Regeneration ◽  
Calcium Phosphate Nanoparticles

Bone-related injury and disease constitute a significant global burden both socially and economically. Current treatments have many limitations and thus the development of new approaches for bone-related conditions is imperative. Gene therapy is an emerging approach for effective bone repair and regeneration, with notable interest in the use of RNA interference (RNAi) systems to regulate gene expression in the bone microenvironment. Calcium phosphate nanoparticles represent promising materials for use as non-viral vectors for gene therapy in bone tissue engineering applications due to their many favorable properties, including biocompatibility, osteoinductivity, osteoconductivity, and strong affinity for binding to nucleic acids. However, low transfection rates present a significant barrier to their clinical use. This article reviews the benefits of calcium phosphate nanoparticles for RNAi delivery and highlights the role of surface functionalization in increasing calcium phosphate nanoparticles stability, improving cellular uptake and increasing transfection efficiency. Currently, the underlying mechanistic principles relating to these systems and their interplay during in vivo bone formation is not wholly understood. Furthermore, the optimal microRNA targets for particular bone tissue regeneration applications are still unclear. Therefore, further research is required in order to achieve the optimal calcium phosphate nanoparticles-based systems for RNAi delivery for bone tissue regeneration.

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