In vitro study of hydroxyapatite-based photocurable polymer composites prepared by laser stereolithography and supercritical fluid extraction

2008 ◽  
Vol 4 (6) ◽  
pp. 1603-1610 ◽  
Author(s):  
John J.A. Barry ◽  
Alexandr V. Evseev ◽  
Mikhail A. Markov ◽  
Clare E. Upton ◽  
Colin A. Scotchford ◽  
...  
Keyword(s):  
Polymer Composites ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fluid Extraction ◽  
Laser Stereolithography

scholarly journals Bioactive Compounds Obtained from Polish “Marynka” Hop Variety Using Efficient Two-Step Supercritical Fluid Extraction and Comparison of Their Antibacterial, Cytotoxic, and Anti-Proliferative Activities In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2366
Author(s):  
Katarzyna Klimek ◽  
Katarzyna Tyśkiewicz ◽  
Malgorzata Miazga-Karska ◽  
Agnieszka Dębczak ◽  
Edward Rój ◽  
...  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Normal Skin ◽  
Extraction Methods ◽  
Biological Properties ◽  
Added Value ◽  
Fluid Extraction

Given the health-beneficial properties of compounds from hop, there is still a growing trend towards developing successful extraction methods with the highest yield and also receiving the products with high added value. The aim of this study was to develop efficient extraction method for isolation of bioactive compounds from the Polish “Marynka” hop variety. The modified two-step supercritical fluid extraction allowed to obtain two hop samples, namely crude extract (E1), composed of α-acids, β-acids, and terpene derivatives, as well as pure xanthohumol with higher yield than that of other available methods. The post-extraction residues (R1) were re-extracted in order to obtain extract E2 enriched in xanthohumol. Then, both samples were subjected to investigation of their antibacterial (anti-acne, anti-caries), cytotoxic, and anti-proliferative activities in vitro. It was demonstrated that extract (E1) possessed more beneficial biological properties than xanthohumol. It exhibited not only better antibacterial activity against Gram-positive bacteria strains (MIC, MBC) but also possessed a higher synergistic effect with commercial antibiotics when compared to xanthohumol. Moreover, cell culture experiments revealed that crude extract neither inhibited viability nor divisions of normal skin fibroblasts as strongly as xanthohumol. In turn, calculated selectivity indexes showed that the crude extract had from slightly to significantly better selective anti-proliferative activity towards cancer cells in comparison with xanthohumol.

scholarly journals The Supercritical Fluid Extraction of Alkaloids from Papaya (Carica papaya L. var. Eksotika) Leaves

1970 ◽  
Vol 4 (2) ◽  
pp. 35-49 ◽  
Author(s):  
Mohamad Fhaizal Mohamad Bukhari ◽  
Noorsaadah Abdul Rahman ◽  
Norzulaani Khalid ◽  
Ahmad Hazri Rashid ◽  
Mazita Mohd Diah
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Carica Papaya ◽  
Extraction Procedure ◽  
Solvent System ◽  
Fluid Extraction ◽  
Suspension Cells ◽  
Fruit Peel ◽  
Pure Compounds

Since the isolation procedures are easy, many of the earliest pure compounds isolated with biological activitywere alkaloids. The nitrogen molecules in plant cells generally make the compound alkaline which makealkaloids exist in a salt form in plants. Thus, alkaloids are often extracted with water and/or acid solution systemand then recovered as crystalline material by treatment using a base. The alkaloid carpaine was extracted fromvarious part of Carica papaya L. var. Eksotika from field grown samples namely leaves, petiole and fruit peel,and from in vitro samples namely leaves, petiole, suspension cells and suspension liquid with only one artifact ofimpurity detected i.e. dehydrocarpaine II. Supercritical fluid extraction was analysed to obtain pure and highyield of carpaine compound as compared to conventional acid base extraction method. The ratio ofethanol/water/acetic acid used at 94.5:5:0.5 (v/v/v) was confirmed to be a better solvent system for carpaineextraction since only one pseudocarpaine i.e. dehydrocarpaine II was extracted together with carpaine. In thisstudy, the application of single fluid of carbon dioxide in supercritical fluid extraction procedure generated pureand higher yield of carpaine compound. Additional centrifugation step should have contributed to a higher purityof the extracted carpaine.

In vitro study of toxicity of hybrid gold-polymer composites

2018 ◽  
Vol 671 (1) ◽  
pp. 1-8 ◽  
Author(s):  
N. Kutsevol ◽  
Yu. Harahuts ◽  
I. Shton ◽  
T. Borikun ◽  
D. Storchai ◽  
...  
Keyword(s):  
Polymer Composites ◽  
In Vitro Study ◽  
Vitro Study
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