Three-dimensional nitrogen-doped mesoporous carbon nanomaterials derived from plant biomass: Cost-effective construction of label-free electrochemical aptasensor for sensitively detecting alpha-fetoprotein

2019 ◽  
Vol 1078 ◽  
pp. 125-134 ◽  
Author(s):  
Xiaoyu Huang ◽  
Bingbing Cui ◽  
Yashen Ma ◽  
Xu Yan ◽  
Lei Xia ◽  
...  
Keyword(s):  
Mesoporous Carbon ◽  
Carbon Nanomaterials ◽  
Plant Biomass ◽  
Three Dimensional ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Nitrogen Doped

Plasma enhanced label-free immunoassay for alpha-fetoprotein based on a U-bend fiber-optic LSPR biosensor

RSC Advances ◽  
2015 ◽  
Vol 5 (31) ◽  
pp. 23990-23998 ◽  
Author(s):  
Gaoling Liang ◽  
Zhongjun Zhao ◽  
Yin Wei ◽  
Kunping Liu ◽  
Wenqian Hou ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Fiber Optic ◽  
Cost Effective ◽  
Alpha Fetoprotein ◽  
Localized Surface Plasmon ◽  
Label Free

A simple, label-free and cost-effective localized surface plasmon resonance (LSPR) immunosensing method was developed for detection of alpha-fetoprotein (AFP).

scholarly journals Harnessing inherently hierarchical microstructures of plant biomass to construct three-dimensional nanoporous nitrogen-doped carbons as efficient and durable oxygen reduction electrocatalysts

RSC Advances ◽  
2019 ◽  
Vol 9 (69) ◽  
pp. 40326-40335 ◽  
Author(s):  
Hongqu Tang ◽  
Shilin Wei ◽  
Chuangchuang Yang ◽  
Peiyao Bai ◽  
Jiawei Qi ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Oxygen Reduction ◽  
Plant Biomass ◽  
Three Dimensional ◽  
Nitrogen Doped ◽  
Metal Free ◽  
Nitrogen Doped Carbon ◽  
Carbon Based

Naturally hierarchical microstructures of E. tirucalli are utilized to prepare a nanoporous nitrogen-doped carbon-based metal-free ORR electrocatalyst that is superior to the benchmark Pt/C catalyst in catalytic activity and durability.

An ultra-sensitive label-free immunosensor toward alpha-fetoprotein detection based on three–dimensional ordered IrOx inverse opals

2018 ◽  
Vol 254 ◽  
pp. 660-668 ◽  
Author(s):  
Kuang Sheng ◽  
Wei Liu ◽  
Lin Xu ◽  
Yandong Jiang ◽  
Xinran Zhang ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Inverse Opals

scholarly journals Three-Dimensional Self-Assembled Nanocarbon For Theranostics Applications

2021 ◽  
Author(s):  
A K M Rezaul Haque Chowdhury
Keyword(s):  
Hela Cells ◽  
Carbon Nanostructures ◽  
Biomedical Applications ◽  
Carbon Nanomaterials ◽  
Three Dimensional ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Diagnostic Applications ◽  
Therapy And Diagnosis

Carbon nanomaterials have been explored for biomedical applications such as scaffolds in tissue engineering, drug delivery carriers, cancer diagnostics and biological imaging. Due to their possible cytotoxicity and biological inertness, they need biological or chemical functionalization to attain biomedical applications. Current research trends are for the synthesis of biocompatible and self-functionalized nanocarbon with prospective application in therapy and diagnosis. The main objectives of this thesis are to synthesize 3D self-functionalized biocompatible nanocarbon for therapeutic and diagnostic applications. The synthesis of the unique three-dimensional carbon nanostructures has been done with ultrashort femtosecond laser processing mechanism, a versatile yet precise technique for nanoscale material generation. First study deals with the synthesis of 3D nanocarbon network and its biocompatibility assessment. Quantitative and qualitative studies of the fibroblast cell response to this nano-network are performed. The findings from the in-vitro study indicate that the platform possesses excellent biocompatibility and promote cell adhesion and subsequent cell proliferation. In next study, the synthesized nanocarbon network (CNRN) platform that possesses a variation in C-C and C-O bond architecture showed dual functionality i. e. cytophilic to fibroblasts but cytotoxic to HeLa cells. Two distict opposite responses like tissue generation for fibroblasts and apoptosis like function for HeLa was observed after 48-hour of culture. The results have potentials or therapeutic appliations. Third study focuses on the diagnostic applications of the nanocarbon. A unique non-plasmonic SERS based bio-sensing platform using 3D nanocarbon is introduced for in-vitro detection and differentiation of HeLa and fibroblast cells. Time based Raman spectroscopy of these cells seeded on nanocarbon revealed chemical fingerprints of intracellular components like DNA/RNA, protein and lipids. Their spectroscopic differences guide differentiation of each cell. Finally, we have synthesized N-enriched nanocarbon probe through nitrogen incorporation-assisted ionization and demonstrate label free SERS based detection of transient variation of cell chemistry and thereby cancer cell diagnosis with N-enriched 3D nanocarbon probe. The results suggested that the SERS functionality not only reveal the chemical fingerprint of the intracellular components (e. g. protein, DNA, RNA etc.) within a cell but also guide detection of cancerous HeLa cells. The results obtained in this thesis point out multifunctional viability of biocompatible self-functionalized nanocarbons for therapy and diagnosis.

A graphene oxide-based label-free electrochemical aptasensor for the detection of alpha-fetoprotein

2018 ◽  
Vol 112 ◽  
pp. 186-192 ◽  
Author(s):  
Shaohong Yang ◽  
Feifei Zhang ◽  
Zonghua Wang ◽  
Qionglin Liang
Keyword(s):  
Graphene Oxide ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Electrochemical Aptasensor

scholarly journals Three-Dimensional Self-Assembled Nanocarbon For Theranostics Applications

2021 ◽  
Author(s):  
A K M Rezaul Haque Chowdhury
Keyword(s):  
Hela Cells ◽  
Carbon Nanostructures ◽  
Biomedical Applications ◽  
Carbon Nanomaterials ◽  
Three Dimensional ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Diagnostic Applications ◽  
Therapy And Diagnosis

Carbon nanomaterials have been explored for biomedical applications such as scaffolds in tissue engineering, drug delivery carriers, cancer diagnostics and biological imaging. Due to their possible cytotoxicity and biological inertness, they need biological or chemical functionalization to attain biomedical applications. Current research trends are for the synthesis of biocompatible and self-functionalized nanocarbon with prospective application in therapy and diagnosis. The main objectives of this thesis are to synthesize 3D self-functionalized biocompatible nanocarbon for therapeutic and diagnostic applications. The synthesis of the unique three-dimensional carbon nanostructures has been done with ultrashort femtosecond laser processing mechanism, a versatile yet precise technique for nanoscale material generation. First study deals with the synthesis of 3D nanocarbon network and its biocompatibility assessment. Quantitative and qualitative studies of the fibroblast cell response to this nano-network are performed. The findings from the in-vitro study indicate that the platform possesses excellent biocompatibility and promote cell adhesion and subsequent cell proliferation. In next study, the synthesized nanocarbon network (CNRN) platform that possesses a variation in C-C and C-O bond architecture showed dual functionality i. e. cytophilic to fibroblasts but cytotoxic to HeLa cells. Two distict opposite responses like tissue generation for fibroblasts and apoptosis like function for HeLa was observed after 48-hour of culture. The results have potentials or therapeutic appliations. Third study focuses on the diagnostic applications of the nanocarbon. A unique non-plasmonic SERS based bio-sensing platform using 3D nanocarbon is introduced for in-vitro detection and differentiation of HeLa and fibroblast cells. Time based Raman spectroscopy of these cells seeded on nanocarbon revealed chemical fingerprints of intracellular components like DNA/RNA, protein and lipids. Their spectroscopic differences guide differentiation of each cell. Finally, we have synthesized N-enriched nanocarbon probe through nitrogen incorporation-assisted ionization and demonstrate label free SERS based detection of transient variation of cell chemistry and thereby cancer cell diagnosis with N-enriched 3D nanocarbon probe. The results suggested that the SERS functionality not only reveal the chemical fingerprint of the intracellular components (e. g. protein, DNA, RNA etc.) within a cell but also guide detection of cancerous HeLa cells. The results obtained in this thesis point out multifunctional viability of biocompatible self-functionalized nanocarbons for therapy and diagnosis.

scholarly journals Three-dimensional self-assembled nanocarbon for theranostics applications

2021 ◽  
Author(s):  
A K M Rezaul Haque Chowdhury
Keyword(s):  
Hela Cells ◽  
Carbon Nanostructures ◽  
Biomedical Applications ◽  
Carbon Nanomaterials ◽  
Three Dimensional ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Diagnostic Applications ◽  
Therapy And Diagnosis

Carbon nanomaterials have been explored for biomedical applications such as scaffolds in tissue engineering, drug delivery carriers, cancer diagnostics and biological imaging. Due to their possible cytotoxicity and biological inertness, they need biological or chemical functionalization to attain biomedical applications. Current research trends are for the synthesis of biocompatible and self-functionalized nanocarbon with prospective application in therapy and diagnosis. The main objectives of this thesis are to synthesize 3D self-functionalized biocompatible nanocarbon for therapeutic and diagnostic applications. The synthesis of the unique three-dimensional carbon nanostructures has been done with ultrashort femtosecond laser processing mechanism, a versatile yet precise technique for nanoscale material generation. First study deals with the synthesis of 3D nanocarbon network and its biocompatibility assessment. Quantitative and qualitative studies of the fibroblast cell response to this nano-network are performed. The findings from the in-vitro study indicate that the platform possesses excellent biocompatibility and promote cell adhesion and subsequent cell proliferation. In next study, the synthesized nanocarbon network (CNRN) platform that possesses a variation in C-C and C-O bond architecture showed dual functionality i. e. cytophilic to fibroblasts but cytotoxic to HeLa cells. Two distict opposite responses like tissue generation for fibroblasts and apoptosis like function for HeLa was observed after 48-hour of culture. The results have potentials for therapeutic appliations. Third study focuses on the diagnostic applications of the nanocarbon. A unique non-plasmonic SERS based bio-sensing platform using 3D nanocarbon is introduced for in-vitro detection and differentiation of HeLa and fibroblast cells. Time based Raman spectroscopy of these cells seeded on nanocarbon revealed chemical fingerprints of intracellular components like DNA/RNA, protein and lipids. Their spectroscopic differences guide differentiation of each cell. Finally, we have synthesized N-enriched nanocarbon probe through nitrogen incorporation-assisted ionization and demonstrate label free SERS based detection of transient variation of cell chemistry and thereby cancer cell diagnosis with N-enriched 3D nanocarbon probe. The results suggested that the SERS functionality not only reveal the chemical fingerprint of the intracellular components (e. g. protein, DNA, RNA etc.) within a cell but also guide detection of cancerous HeLa cells. The results obtained in this thesis point out multifunctional viability of biocompatible self-functionalized nanocarbons for therapy and diagnosis.

A novel three-dimensional DNA nanostructure used for thrombin detection

2017 ◽  
Vol 9 (35) ◽  
pp. 5115-5120 ◽  
Author(s):  
Huawei Shu ◽  
He Mei ◽  
Jinxin Gu ◽  
Yongbin Yang ◽  
Baoguang Chen ◽  
...  
Keyword(s):  
Three Dimensional ◽  
High Sensitivity ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure

In this paper, a label-free electrochemical aptasensor for the detection of thrombin with high sensitivity is proposed.

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