Functional chimera aptamer and molecular beacon based fluorescent detection of Staphylococcus aureus with strand displacement-target recycling amplification

2019 ◽  
Vol 1075 ◽  
pp. 128-136 ◽  
Author(s):  
Rongfeng Cai ◽  
Fan Yin ◽  
Zhongwen Zhang ◽  
Yaping Tian ◽  
Nandi Zhou
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Beacon ◽  
Fluorescent Detection ◽  
Strand Displacement ◽  
Target Recycling ◽  
Recycling Amplification

scholarly journals Aptamer Conformation Switching-Induced Two-Stage Amplification for Fluorescent Detection of Proteins

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 77 ◽  
Author(s):  
Qiao Yu ◽  
Fenfen Zhai ◽  
Hong Zhou ◽  
Zonghua Wang
Keyword(s):  
Molecular Beacon ◽  
Limit Of Detection ◽  
Low Cost ◽  
Dna Aptamer ◽  
Fluorescent Detection ◽  
Fluorescence Signal ◽  
Sensing Platform ◽  
Low Concentrations ◽  
Diagnostic Applications ◽  
Recycling Amplification

Basing on the conformation change of aptamer caused by proteins, a simple and sensitive protein fluorescent assay strategy is proposed, which is assisted by the isothermal amplification reaction of polymerase and nicking endonuclease. In the presence of platelet-derived growth factor (PDGF-BB), the natural conformation of a DNA aptamer would change into a Y-shaped complex, which could hybridize with a molecular beacon (MB) and form a DNA duplex, leading to the open state of the MB and generating a fluorescence signal. Subsequently, with further assistance of isothermal recycling amplification strategies, the designed aptamer sensing platform showed an increment of fluorescence. As a benefit of this amplified strategy, the limit of detection (LOD) was lowered to 0.74 ng/mL, which is much lower than previous reports. This strategy not only offers a new simple, specific, and efficient platform to quantify the target protein in low concentrations, but also shows a powerful approach without multiple washing steps, as well as a precious implementation that has the potential to be integrated into portable, low-cost, and simplified devices for diagnostic applications.

A sensitive biosensing strategy for DNA detection based on graphene oxide and T7 exonuclease assisted target recycling amplification

2013 ◽  
Vol 91 (12) ◽  
pp. 1266-1271 ◽  
Author(s):  
Hai-Bo Wang ◽  
Li-Juan Ou ◽  
Ke-Jing Huang ◽  
Xin-Ge Wen ◽  
Ling-Ling Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Detection Sensitivity ◽  
Target Recycling ◽  
Target Dna ◽  
Quenching Efficiency ◽  
Signal Probe ◽  
Recycling Amplification ◽  
T7 Exonuclease

A fluorescence biosensing strategy based on graphene oxide (GO) was reported for simple, rapid, sensitive, and selective DNA detection by T7 exonuclease assisted target recycling amplification. Due to the super fluorescence quenching efficiency of GO, the fluorescein amiditelabeled signal probe was firstly adsorbed onto the surface of GO and the fluorescence was quenched. Owing to its excellent selectivity for double-stranded DNA, T7 exonuclease was chosen as a signal-amplifying biocatalyst to improve the detection sensitivity. In the presence of target DNA, the signal probe could bind with target DNA and form a DNA duplex structure to trigger the digestion of the signal probe by T7 exonuclease, leading to the recycling of target DNA and the increasing of fluorescence intensity. Upon the recycling use of target DNA, this method achieved a high sensitivity towards target DNA with a detection limit of 0.3 pmol/L, which was lower than previously reported for GO-based DNA biosensors. Moreover, it does not require complex modifications of the molecular beacon and time-consuming thermal cycling procedures. Thus, the simple strategy provides a universal biosensing platform for DNA detection and it could find wide applications in DNA damage analysis and diagnostics.

A simple molecular beacon with duplex-specific nuclease amplification for detection of microRNA

The Analyst ◽  
2016 ◽  
Vol 141 (3) ◽  
pp. 1071-1076 ◽  
Author(s):  
Yingcun Li ◽  
Jiangyan Zhang ◽  
Jingjing Zhao ◽  
Likun Zhao ◽  
Yongqiang Cheng ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Target Recycling ◽  
Amplification Method ◽  
Microrna Detection ◽  
Recycling Amplification

A target recycling amplification method based on a simple molecular beacon and duplex-specific nuclease has been developed for microRNA detection.

Target–aptamer binding triggered quadratic recycling amplification for highly specific and ultrasensitive detection of antibiotics at the attomole level

2015 ◽  
Vol 51 (39) ◽  
pp. 8377-8380 ◽  
Author(s):  
Hongzhi Wang ◽  
Yu Wang ◽  
Su Liu ◽  
Jinghua Yu ◽  
Wei Xu ◽  
...  
Keyword(s):  
Ultrasensitive Detection ◽  
Electrochemical Aptasensor ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Target Recycling ◽  
Nicking Endonuclease ◽  
Recycling Amplification

A novel electrochemical aptasensor for ultrasensitive detection of antibiotics by combining polymerase-assisted target recycling amplification with strand displacement amplification with the help of polymerase and nicking endonuclease has been reported.

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