Label-free counting of Escherichia coli cells in nanoliter droplets using 3D printed microfluidic devices with integrated contactless conductivity detection

2019 ◽  
Vol 1071 ◽  
pp. 36-43 ◽  
Author(s):  
Lucas C. Duarte ◽  
Federico Figueredo ◽  
Luiz E.B. Ribeiro ◽  
Eduardo Cortón ◽  
Wendell K.T. Coltro
Keyword(s):  
Escherichia Coli ◽  
Microfluidic Devices ◽  
Label Free ◽  
Conductivity Detection ◽  
Contactless Conductivity Detection ◽  
Escherichia Coli Cells ◽  
3D Printed

Microfluidic devices with integrated dual-capacitively coupled contactless conductivity detection to monitor binding events in real time

2014 ◽  
Vol 192 ◽  
pp. 239-246 ◽  
Author(s):  
Wendell Karlos Tomazelli Coltro ◽  
Rodrigo de Santis Neves ◽  
Artur de Jesus Motheo ◽  
José Alberto Fracassi da Silva ◽  
Emanuel Carrilho
Keyword(s):  
Real Time ◽  
Microfluidic Devices ◽  
Conductivity Detection ◽  
Capacitively Coupled ◽  
Contactless Conductivity Detection

DETECTION OF GAMMA RADIATION EFFECT INDUCED BY COBELT-60 ON ESCHERICHIA COLI CELLS

2010 ◽  
Vol 13 (3) ◽  
pp. 129-133
Author(s):  
Ghusoon Ali Abdul Hasan Al-Sudany ◽  
◽  
Wasan Zuheir Majeed ◽  
Hind Jabbar Abdul Rhman Akram Al-Aubeidi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gamma Radiation ◽  
Radiation Effect ◽  
Escherichia Coli Cells

scholarly journals Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Huiyi Song ◽  
Ni Lou ◽  
Jianjun Liu ◽  
Hong Xiang ◽  
Dong Shang
Keyword(s):  
Escherichia Coli ◽  
Proteomic Analysis ◽  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Label Free ◽  
Quantitative Proteomic Analysis ◽  
E Coli ◽  
Protein Ubiquitination

Abstract Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance. This study employed proteomic analysis to identify differentially expressed proteins after coculture of E. coli with Lactobacillus rhamnosus GG (LGG) microcapsules. Methods To explore the relevant protein abundance changes after E. coli and LGG coculture, label-free quantitative proteomic analysis and qRT-PCR were applied to E. coli and LGG microcapsule groups before and after coculture, respectively. Results The proteomic analysis characterised a total of 1655 proteins in E. coli K12MG1655 and 1431 proteins in the LGG. After coculture treatment, there were 262 differentially expressed proteins in E. coli and 291 in LGG. Gene ontology analysis showed that the differentially expressed proteins were mainly related to cellular metabolism, the stress response, transcription and the cell membrane. A protein interaction network and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentiated proteins were mainly involved in the protein ubiquitination pathway and mitochondrial dysfunction. Conclusions These findings indicated that LGG microcapsules may inhibit E. coli biofilm formation by disrupting metabolic processes, particularly in relation to energy metabolism and stimulus responses, both of which are critical for the growth of LGG. Together, these findings increase our understanding of the interactions between bacteria under coculture conditions.

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