Towards a high peak capacity of 130 using nanoflow hydrophilic interaction liquid chromatography

2019 ◽  
Vol 1062 ◽  
pp. 147-155 ◽  
Author(s):  
Ya Liu ◽  
Xiaofei Wang ◽  
Zhangqian Chen ◽  
Derek Hecheng Liang ◽  
Kaiyue Sun ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Peak ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Peak Capacity ◽  
Hydrophilic Interaction

Aminoquinolines as fluorescent labels for hydrophilic interaction liquid chromatography of oligosaccharides

2012 ◽  
Vol 393 (8) ◽  
pp. 757-765 ◽  
Author(s):  
Weston B. Struwe ◽  
Pauline M. Rudd
Keyword(s):  
Liquid Chromatography ◽  
Hplc Analysis ◽  
Three Dimensional ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Peak Capacity ◽  
Fluorescent Labels ◽  
Sample Analysis ◽  
Hydrophilic Interaction ◽  
Optimal Excitation ◽  
Complex Sample

Abstract In this study, we investigated the potential of four different aminoquinoline (AQ) compounds as fluorescent labels for glycan analysis using hydrophilic interaction liquid chromatography (HILIC) and fluorescence detection (FLD). We confirmed the optimal excitation and emission wavelengths of 3-AQ and 6-AQ conjugated to glycan standards using three-dimensional fluorescent spectral scanning. The optimal excitation and emission wavelengths for 6-AQ were confirmed at λex=355 nm and λem=440 nm. We concluded that the optimal wavelengths for 3-AQ were λex=355 nm and λem=420 nm, which differed considerably from the wavelengths applied in previous reports. HILIC-FLD chromatograms using experimentally determined wavelengths were similar to 2-aminobenzamide controls, but the peak capacity and resolution differed significantly when published 3-AQ λex/em values were applied. Furthermore, we found that 5-AQ and 8-AQ labeled maltohexaose did not display any fluorescent pro\xadperties when used as a carbohydrate tag for HPLC analysis. Finally, we applied experimentally determined wavelengths to 3-AQ labeled N-glycans released from human IgG to illustrate changes in retention time as well as to demonstrate that AQ labeling is applicable to complex sample analysis via exoglycosidase sequencing.

Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations

2016 ◽  
Vol 8 (31) ◽  
pp. 6038-6045 ◽  
Author(s):  
Yadi Wang ◽  
M. Farooq Wahab ◽  
Zachary S. Breitbach ◽  
Daniel W. Armstrong
Keyword(s):  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Stationary Phase ◽  
Mixed Mode ◽  
High Efficiency ◽  
Stationary Phases ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Stationary phases composed of native cyclofructan 6 (CF6) and benzoic acid modified CF6 were synthesized and evaluated for hydrophilic interaction liquid chromatography (HILIC).

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