Combination of field amplified sample injection and hydrophobic interaction electrokinetic chromatography (FASI-HIEKC) as a signal amplification method for the determination of selected macrocyclic antibiotics

2019 ◽  
Vol 1046 ◽  
pp. 192-198 ◽  
Author(s):  
Piotr Kowalski ◽  
Ilona Olędzka ◽  
Alina Plenis ◽  
Natalia Miękus ◽  
Michał Pieckowski ◽  
...  
Keyword(s):  
Hydrophobic Interaction ◽  
Signal Amplification ◽  
Electrokinetic Chromatography ◽  
Sample Injection ◽  
Amplification Method ◽  
Macrocyclic Antibiotics

Simultaneous determination of ingredients in an ointment by hydrophobic interaction electrokinetic chromatography

2001 ◽  
Vol 929 (1-2) ◽  
pp. 133-141 ◽  
Author(s):  
Hitoshi Okamoto ◽  
Atsuhiro Uetake ◽  
Ryuji Tamaya ◽  
Toshiaki Nakajima ◽  
Kazuhiko Sagara ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Hydrophobic Interaction ◽  
Electrokinetic Chromatography

scholarly journals A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

1995 ◽  
Vol 43 (4) ◽  
pp. 347-352 ◽  
Author(s):  
H M Kerstens ◽  
P J Poddighe ◽  
A G Hanselaar
Keyword(s):  
In Situ Hybridization ◽  
Signal Amplification ◽  
Dna Probes ◽  
Bright Field ◽  
Archival Material ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Catalyzed Reporter Deposition

For amplification of in situ hybridization (ISH) signals, we describe a method using catalyzed reporter deposition (CARD). This amplification method is based on the deposition of biotinylated tyramine (BT) at the location of the DNA probe. The BT precipitate can then visualized with fluorochrome- or enzyme-labeled avidin. Both for bright-field ISH (BRISH) and for fluorescence ISH (FISH), the detection limit was highly increased. This method is especially suitable for visualization of very weak ISH signals, such as those obtained by ISH using locus-specific DNA probes. Furthermore, CARD amplification of ISH signals (CARD-ISH) is highly sensitive, rapid, flexible, and easy to implement. Successful application of CARD-ISH with locus-specific DNA probes on histological and cytological samples may improve the determination of structural chromosomal aberrations in archival material.

A new photoelectrochemical biosensor for ultrasensitive determination of nucleic acids based on a three-stage cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2799-2806 ◽  
Author(s):  
Erhu Xiong ◽  
Xiaoxia Yan ◽  
Xiaohua Zhang ◽  
Yanmei Li ◽  
Ruiying Yang ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Dna Detection ◽  
Amplification Method ◽  
Photoelectrochemical Biosensor ◽  
Amplification Strategy

Based on a “signal-on” sensing strategy and a three-stage cascade signal amplification method, an ultrasensitive photoelectrochemical biosensor has been developed for DNA detection.

scholarly journals An LC-MS/MS method for protein detection based on a mass barcode and dual-target recognition strategy

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 16094-16100
Author(s):  
Duo Li ◽  
Qinxin Song ◽  
Tengfei Li ◽  
Chang Shu ◽  
Shunli Ji ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Target Recognition ◽  
Protein Detection ◽  
Amplification Method ◽  
Dual Target

A mass barcode-mediated signal amplification method was developed for the determination of protein by LC-MS/MS.

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