Low-cost and disposable sensors for the simultaneous determination of coenzyme Q10 and α-lipoic acid using manganese (IV) oxide-modified screen-printed graphene electrodes

2018 ◽  
Vol 1004 ◽  
pp. 22-31 ◽  
Author(s):  
Kanokwan Charoenkitamorn ◽  
Sudkate Chaiyo ◽  
Orawon Chailapakul ◽  
Weena Siangproh
Keyword(s):  
Simultaneous Determination ◽  
Lipoic Acid ◽  
Coenzyme Q10 ◽  
Low Cost ◽  
Screen Printed

Disposable screen-printed chemiluminescent biochips for the simultaneous determination of four point-of-care relevant proteins

2008 ◽  
Vol 393 (4) ◽  
pp. 1191-1198 ◽  
Author(s):  
C. A. Marquette ◽  
F. Bouteille ◽  
B. P. Corgier ◽  
A. Degiuli ◽  
L. J. Blum
Keyword(s):  
Simultaneous Determination ◽  
Point Of Care ◽  
Screen Printed

Environmentally Evaluated New HPLC/UV Method for the Simultaneous Determination of Acesulfame-K, Butylated Hydroxytoluene, and Aspartame and Its Degradant in Chewing Gum

2019 ◽  
Vol 102 (6) ◽  
pp. 1892-1900
Author(s):  
Nada S. Zamzam ◽  
Mona H. Abdel Rahman ◽  
Maha F. Abdel Ghani
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Low Cost ◽  
Food Additives ◽  
Gradient Elution ◽  
Butylated Hydroxytoluene ◽  
Chewing Gum ◽  
Adverse Health Effects ◽  
Chewing Gums

Background: Acesulfame-K (ACE), butylated hydroxytoluene (BHT), and aspartame (ASP) are a common combination of food additives added to chewing gums. The abuse of these additives results in severe adverse health effects; however, they are still extensively used owing to their high performance and low cost. Objective: The development and optimization of a simple, cheap, sensitive, and eco-friendly HPLC/UV method for the simultaneous determination of ASP, ACE, and BHT along with aspartame degradation product phenylalanine (PHEN) in chewing gum. Methods: The method was optimized using a 5 μm C18 column and an eluent consisting of methanol and 0.1 M phosphate buffer (pH 5.0) according to a suitable gradient elution program. Simple sample preparation, consisting of dilution, homogenization, and sonication followed by centrifugation and filtration, was optimized and used for the extraction of chewing gum. The greenness of the method was evaluated. Results: The proposed method exhibited excellent linearity (R2 > 0.9996), low LOQ (0.08–0.95 μg/mL), and recoveries between 85.3 and 98.83% with relative SD (RSD) ≤ 2.7%. High resolution was obtained with <25 min run times with excellent precision (RSD: 0.28–1.33%). This method was successfully applied for the simultaneous determination of ACE, ASP, and BHT in commercial chewing gum; PHEN was not detected. Furthermore, our method is considered to be environmentally acceptable. Conclusions: The results demonstrate that the developed method can be used to detect ACE, BHT, ASP, and PHEN in chewing gum. Highlights: A new sensitive, green HPLC/UV method is developed to be used as a minimal-cost routine analysis procedure for commercial chewing gum.

Environmentally Evaluated New HPLC/UV Method for the Simultaneous Determination of Acesulfame-K, Butylated Hydroxytoluene, and Aspartame and Its Degradant in Chewing Gum

2019 ◽  
Vol 102 (6) ◽  
pp. 1892-1900
Author(s):  
Nada S Zamzam ◽  
Mona H Abdel Rahman ◽  
Maha F Abdel Ghani
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Low Cost ◽  
Food Additives ◽  
Gradient Elution ◽  
Butylated Hydroxytoluene ◽  
Chewing Gum ◽  
Adverse Health Effects ◽  
Chewing Gums

Abstract Background: Acesulfame-K (ACE), butylated hydroxytoluene (BHT), and aspartame (ASP) are a common combination of food additives added to chewing gums. The abuse of these additives results in severe adverse health effects; however, they are still extensively used owing to their high performance and low cost. Objective: The development and optimization of a simple, cheap, sensitive, and eco-friendly HPLC/UV method for the simultaneous determination of ASP, ACE, and BHT along with aspartame degradation product phenylalanine (PHEN) in chewing gum. Methods: The method was optimized using a 5 μm C18 column and an eluent consisting of methanol and 0.1 M phosphate buffer (pH 5.0) according to a suitable gradient elution program. Simple sample preparation, consisting of dilution, homogenization, and sonication followed by centrifugation and filtration, was optimized and used for the extraction of chewing gum. The greenness of the method was evaluated. Results: The proposed method exhibited excellent linearity (R2 &gt; 0.9996), low LOQ (0.08–0.95 μg/mL), and recoveries between 85.3 and 98.83% with relative SD (RSD) ≤ 2.7%. High resolution was obtained with &lt;25 min run times with excellent precision (RSD: 0.28–1.33%). This method was successfully applied for the simultaneous determination of ACE, ASP, and BHT in commercial chewing gum; PHEN was not detected. Furthermore, our method is considered to be environmentally acceptable. Conclusions: The results demonstrate that the developed method can be used to detect ACE, BHT, ASP, and PHEN in chewing gum. Highlights: A new sensitive, green HPLC/UV method is developed to be used as a minimal-cost routine analysis procedure for commercial chewing gum.

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