An entropy-driven signal amplifying strategy for real-time monitoring of DNA methylation process and high-throughput screening of methyltransferase inhibitors

2017 ◽  
Vol 970 ◽  
pp. 57-63 ◽  
Author(s):  
Siyi Chen ◽  
Huan Ma ◽  
Wang Li ◽  
Zhou Nie ◽  
Shouzhuo Yao
Keyword(s):  
Dna Methylation ◽  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Real Time Monitoring

In-situ nanoelectrospray for high-throughput screening of enzymes and real-time monitoring of reactions

2016 ◽  
Vol 902 ◽  
pp. 135-141 ◽  
Author(s):  
Yuhan Yang ◽  
Feifei Han ◽  
Jin Ouyang ◽  
Yunling Zhao ◽  
Juan Han ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Real Time Monitoring

scholarly journals Development of Neh2-Luciferase Reporter and Its Application for High Throughput Screening and Real-Time Monitoring of Nrf2 Activators

2011 ◽  
Vol 18 (6) ◽  
pp. 752-765 ◽  
Author(s):  
Natalya A. Smirnova ◽  
Renee E. Haskew-Layton ◽  
Manuela Basso ◽  
Dmitry M. Hushpulian ◽  
Jimmy B. Payappilly ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening ◽  
Luciferase Reporter ◽  
Real Time Monitoring

Volume Cytometry:  Microfluidic Sensor for High-Throughput Screening in Real Time

2005 ◽  
Vol 77 (5) ◽  
pp. 1290-1294 ◽  
Author(s):  
Daniel A. Ateya ◽  
Frederick Sachs ◽  
Philip A. Gottlieb ◽  
Steve Besch ◽  
Susan Z. Hua
Keyword(s):  
Real Time ◽  
High Throughput ◽  
High Throughput Screening

High-Throughput and Real-Time Monitoring of Single-Cell Extracellular pH Based on Polyaniline Microarrays

2021 ◽  
Author(s):  
Lihui Yang ◽  
Xiaobo Liu ◽  
Bing Yin ◽  
Xunxun Deng ◽  
Xiaotong Lin ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
High Throughput ◽  
Extracellular Ph ◽  
Real Time Monitoring

Real-Time Monitoring of Luciferase-Tagged Cronobacter sakazakii in Reconstituted Infant Milk Formula

2011 ◽  
Vol 74 (4) ◽  
pp. 573-579 ◽  
Author(s):  
RUTH MORRISSEY ◽  
MÁIRE BEGLEY ◽  
SATORU OSHIMA ◽  
MARY REA ◽  
R. PAUL ROSS ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Skim Milk ◽  
Luria Bertani ◽  
Milk Formula ◽  
Cronobacter Sakazakii ◽  
Real Time Monitoring ◽  
Luria Bertani Broth ◽  
Infant Milk Formula

The aim of this study was to examine the potential of using a lux-tagged Cronobacter sakazakii strain to monitor growth of the bacterium in various liquids. C. sakazakii was transformed with plasmid p16Slux, and integration of the plasmid at the desired site on the chromosome was confirmed by PCR. The growth of the lux-tagged strain was similar to that of the non–lux-tagged strain, and the integrated plasmid was stable when cells were cultured in the absence of antibiotic. Growth of the lux-tagged strain was monitored in real time in Luria-Bertani broth, skim milk, and infant milk formula by using both the Luminoskan luminometer and the Xenogen IVIS imager. Bioluminescence could be detected when the lux-tagged strain was cocultured with other bacteria. The effect of monocaprylin and nisin on the growth of C. sakazakii in milk was monitored by measuring bioluminescence. In conclusion, growth of a lux-tagged C. sakazakii can be monitored in real time in both clear and opaque liquids by measuring bioluminescence. lux-tagged C. sakazakii strains could be potentially used in high-throughput assays to monitor the effects of various infant milk formula compositions on growth of the bacterium.

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