scholarly journals A multi-analytical approach to better assess the keratan sulfate contamination in animal origin chondroitin sulfate

2017 ◽  
Vol 958 ◽  
pp. 59-70 ◽  
Author(s):  
Odile Francesca Restaino ◽  
Rosario Finamore ◽  
Paola Diana ◽  
Mariacarmela Marseglia ◽  
Mario Vitiello ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Analytical Approach ◽  
Keratan Sulfate ◽  
Animal Origin

scholarly journals Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 737
Author(s):  
Antonietta Stellavato ◽  
Odile Francesca Restaino ◽  
Valentina Vassallo ◽  
Elisabetta Cassese ◽  
Rosario Finamore ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Matrix Protein ◽  
Structural Characteristics ◽  
Keratan Sulfate ◽  
Pharmaceutical Products ◽  
Animal Origin ◽  
Molecular Weights ◽  
Hyaluronate Synthase ◽  
Inflammatory Effect

The biological activity of chondroitin sulfate (CS) and glucosamine (GlcN) food supplements (FS), sold in USA against osteoarthritis, might depend on the effective CS and GlcN contents and on the CS structural characteristics. In this paper three USA FS were compared to two pharmaceutical products (Ph). Analyses performed by HPAE-PAD, by HPCE and by SEC-TDA revealed that the CS and GlcN titers were up to −68.8% lower than the contents declared on the labels and that CS of mixed animal origin and variable molecular weights was present together with undesired keratan sulfate. Simulated gastric and intestinal digestions were performed in vitro to evaluate the real CS amount that may reach the gut as biopolymer. Chondrocytes and synoviocytes primary cells derived from human pathological joints were used to assess: cell viability, modulation of the NF-κB, quantification of cartilage oligomeric matrix protein (COMP-2), hyaluronate synthase enzyme (HAS-1), pentraxin (PTX-3) and the secreted IL-6 and IL-8 to assess inflammation. Of the three FS tested only one (US FS1) enhanced chondrocytes viability, while all of them supported synoviocytes growth. Although US FS1 proved to be less effective than Ph as it reduced NF-kB, it could not down-regulate COMP-2; HAS-1 was up-regulated but with a lower efficacy. Inflammatory cytokines were markedly reduced by Ph while a slight decrease was only found for US-FS1.

Immunocytochemical Study of Proteoglycans in Vocal Folds

1996 ◽  
Vol 105 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Agnieszka S. Pawlak ◽  
Elizabeth Hammond ◽  
Thomas Hammond ◽  
Steven D. Gray
Keyword(s):  
Chondroitin Sulfate ◽  
Lamina Propria ◽  
Vocal Fold ◽  
Keratan Sulfate ◽  
Vocal Folds ◽  
Basement Membrane Zone ◽  
Immunocytochemical Study ◽  
Frozen Sections ◽  
Intense Staining ◽  
Immunocytochemical Techniques

We evaluated the proteoglycan composition of normal vocal folds using immunocytochemical techniques. Frozen sections of 14 normal cadaveric vocal folds were obtained within 12 hours of death and sectioned immediately. Vocal fold sections were stained with antibodies against keratan sulfate, chondroitin sulfate, heparan sulfate proteoglycan (HSPG), decorin, and hyaluronate receptor. We found that the lamina propria has diffuse staining of fibrillar components with keratan sulfate and decorin. Intense staining was observed in the vocal ligament area with keratan sulfate. The HSPG was localized to the basement membrane zone. Chondroitin sulfate, HSPG, and hyaluronate receptor were detected in the cytoplasm of interstitial cells with immunocytochemical characteristics of macrophages. The keratan sulfate distribution suggests that fibromodulin may be significant in normal vocal folds. Production of HSPG and probably versican occurs in macrophages and fibroblasts in the lamina propria.

Transforming growth factor-β1 upregulates keratan sulfate and chondroitin sulfate biosynthesis in microglias after brain injury

2009 ◽  
Vol 1263 ◽  
pp. 10-22 ◽  
Author(s):  
Jiarong Yin ◽  
Kazuma Sakamoto ◽  
Haoqian Zhang ◽  
Zenya Ito ◽  
Shiro Imagama ◽  
...  
Keyword(s):  
Brain Injury ◽  
Growth Factor ◽  
Chondroitin Sulfate ◽  
Transforming Growth Factor ◽  
Keratan Sulfate ◽  
Transforming Growth Factor Β1

scholarly journals Contributions of Chondroitin Sulfate, Keratan Sulfate and N-linked Oligosaccharides to Inhibition of Neurite Outgrowth by Aggrecan

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 29 ◽  
Author(s):  
Thomas M. Hering ◽  
Justin A. Beller ◽  
Christopher M. Calulot ◽  
Diane M. Snow
Keyword(s):  
Chondroitin Sulfate ◽  
Neurite Outgrowth ◽  
Keratan Sulfate ◽  
Cns Injury ◽  
Post Translational Modifications ◽  
The Central Nervous System ◽  
Quantitative Analyses ◽  
Sequential Digestion ◽  
High Throughput Assay ◽  
First Time

The role of proteoglycans in the central nervous system (CNS) is a rapidly evolving field and has major implications in the field of CNS injury. Chondroitin sulfate proteoglycans (CSPGs) increase in abundance following damage to the spinal cord and inhibit neurite outgrowth. Major advances in understanding the interaction between outgrowing neurites and CSPGs has created a need for more robust and quantitative analyses to further our understanding of this interaction. We report the use of a high-throughput assay to determine the effect of various post-translational modifications of aggrecan upon neurite outgrowth from NS-1 cells (a PC12 cell line derivative). Aggrecan contains chondroitin sulfate, keratan sulfate, and N-linked oligosaccharides (N-glycans), each susceptible to removal through different enzymatic digestions. Using a sequential digestion approach, we found that chondroitin sulfate and N-glycans, but not keratan sulfate, contribute to inhibition of neurite outgrowth by substrate-bound aggrecan. For the first time, we have shown that N-linked oligosaccharides on aggrecan contribute to its inhibition of neuritogenesis.

Unraveling metabolism heterogeneity in colorectal cancer and its implications in pan-cancer cohort.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16016-e16016
Author(s):  
Qijing Wu ◽  
Qiong Huang ◽  
Mengting Sun ◽  
Bishan Liang ◽  
Xingbin Hu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Chondroitin Sulfate ◽  
Molecular Subtype ◽  
Keratan Sulfate ◽  
Good Prognosis ◽  
Nucleotide Metabolism ◽  
Consensus Clustering ◽  
Pyrimidine Synthesis ◽  
Pre Treatment ◽  
Pan Cancer

e16016 Background: Colorectal cancer (CRC) molecular subtype has been emphasized and links to biological and clinical behavior. However, comprehensive metabolism of CRC has not been characterized. Subtype-specific metabolic differences and immunometabolism phenotypes remain unclear. Therefore, this study performed metabolism clustering and explore its relation with immune phenotypes as well as prognosis predictive value. Methods: Transcriptome of TCGA CRC cohort was utilized to generate 113 KEGG metabolism pathway scores via GSVA algorithm. Consensus clustering were used to identify metabolism subtypes. Bioinformatic algorithms were applied in classification and survival analysis. Results: Energy metabolism-oriented subtype, featured by nitrogen metabolism and immune-inflamed, correlated with good prognosis (log rank P < 0.001). Pyrimidine synthesis activated most in nucleotide metabolism-oriented subtype, which was immune-deserted. Stroma metabolism-oriented subtype, characterized by glycosaminoglycan (GAG) biosynthesis and immune-suppressive phenotype, was an independent risk factor for overall survival (multivariate Cox: HR = 2.30 95% CI 1.23-4.28, P = 0.009). Clinical cohort from Nanfang Hospital composed of RNA-Seq data from 26 pre- and post-treatment samples in mCRC patients receiving mFOLFOX+Bevacizumab were classified. 22 were identified and 8 of them were pre-treatment, 4 of which were nucleotide subtype being 100% PR. The rest were stroma subtype with SD or PD, indicating drug resistance in stroma subtype. Interestingly, GAG chondroitin sulfate featured in stroma subtype outperformed other metabolisms in predicting negative prognosis (HR = 1.42 95% CI 1.18-1.71, P < 0.001). We then tested GAG metabolism in TCGA pan-cancer cohort. Chondroitin sulfate and heparan sulfate metabolism subtype correlated inversely with bladder (HR = 1.23 95% CI 1.06-1.43, P = 0.007) and breast cancer (HR = 1.27 95% CI 1.10-1.47, P = 0.001) patients’ survival, respectively. Keratan sulfate subtype predicted worse survival in glioblastoma, pancreatic and liver cancer (HR = 1.31 95% CI 1.09-1.57, P = 0.003; HR = 1.31 95% CI 1.06-1.61, P = 0.01; HR = 1.23 95% CI 1.04-1.46, P = 0.02). These indicated a pan-cancer relevant role of GAG disorder. Conclusions: CRC metabolism subtypes unraveled metabolism heterogeneity with prognosis value. CRC stroma subtype and other stroma-involved cancers share active GAG metabolism, which may lighten common targeting strategies in multiple stroma-accompanied cancers.

Chondroitin sulfate and keratan sulfate are the major glycosaminoglycans present in the adult zebrafish Danio rerio (Chordata-Cyprinidae)

2007 ◽  
Vol 24 (9) ◽  
pp. 521-530 ◽  
Author(s):  
Aline R. C. Souza ◽  
Eliene O. Kozlowski ◽  
Vinicius R. Cerqueira ◽  
Morgana T. L. Castelo-Branco ◽  
Manoel L. Costa ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Danio Rerio ◽  
Keratan Sulfate ◽  
Adult Zebrafish
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