Urinary exposure marker discovery for toxicants using ultra-high pressure liquid chromatography coupled with Orbitrap high resolution mass spectrometry and three untargeted metabolomics approaches

2016 ◽  
Vol 939 ◽  
pp. 73-83 ◽  
Author(s):  
Jen-Yi Hsu ◽  
Jing-Fang Hsu ◽  
Yet-Ran Chen ◽  
Chia-Lang Shih ◽  
Yi-Sheng Hsu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Pressure Liquid Chromatography ◽  
High Resolution Mass Spectrometry ◽  
Untargeted Metabolomics ◽  
Ultra High Pressure ◽  
Marker Discovery ◽  
Resolution Mass

scholarly journals Discovery of Pancreatic Adenocarcinoma Biomarkers by Untargeted Metabolomics

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1002 ◽  
Author(s):  
Ariadna Martín-Blázquez ◽  
Cristina Jiménez-Luna ◽  
Caridad Díaz ◽  
Joaquina Martínez-Galán ◽  
Jose Prados ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Untargeted Metabolomics ◽  
Ductal Adenocarcinoma ◽  
Serum Samples ◽  
High Resolution Mass ◽  
Negative Electrospray Ionization ◽  
Resolution Mass

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and lethal cancers, with a 5-year survival rate of less than 5%. In fact, complete surgical resection remains the only curative treatment. However, fewer than 20% of patients are candidates for surgery at the time of presentation. Hence, there is a critical need to identify diagnostic biomarkers with potential clinical utility in this pathology. In this context, metabolomics could be a powerful tool to search for new robust biomarkers. Comparative metabolomic profiling was performed in serum samples from 59 unresectable PDAC patients and 60 healthy controls. Samples were analyzed by using an untargeted metabolomics workflow based on liquid chromatography, coupled to high-resolution mass spectrometry in positive and negative electrospray ionization modes. Univariate and multivariate analysis allowed the identification of potential candidates that were significantly altered in PDAC patients. A panel of nine candidates yielded excellent diagnostic capacities. Pathway analysis revealed four altered pathways in our patients. This study shows the potential of liquid chromatography coupled to high-resolution mass spectrometry as a diagnostic tool for PDAC. Furthermore, it identified novel robust biomarkers with excellent diagnostic capacities.

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