Chemiluminescence imaging for microRNA detection based on cascade exponential isothermal amplification machinery

2016 ◽  
Vol 936 ◽  
pp. 229-235 ◽  
Author(s):  
Yongjie Xu ◽  
Dandan Li ◽  
Wei Cheng ◽  
Rong Hu ◽  
Ye Sang ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Microrna Detection

Rapid MicroRNA Detection Using Paper-Based Isothermal Amplification

2021 ◽  
Author(s):  
Jingjing Qian ◽  
Qinming Zhang ◽  
Joyce C. Lai ◽  
Yixuan Wang ◽  
Meng Lu
Keyword(s):  
Isothermal Amplification ◽  
Microrna Detection

Isothermal Amplification for MicroRNA Detection: From the Test Tube to the Cell

2017 ◽  
Vol 50 (4) ◽  
pp. 1059-1068 ◽  
Author(s):  
Ruijie Deng ◽  
Kaixiang Zhang ◽  
Jinghong Li
Keyword(s):  
Test Tube ◽  
Isothermal Amplification ◽  
Microrna Detection

Attomolar Ultrasensitive MicroRNA Detection by DNA-Scaffolded Silver-Nanocluster Probe Based on Isothermal Amplification

2012 ◽  
Vol 84 (12) ◽  
pp. 5165-5169 ◽  
Author(s):  
Yu-Qiang Liu ◽  
Min Zhang ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Isothermal Amplification ◽  
Silver Nanocluster ◽  
Microrna Detection

scholarly journals Isothermal digital detection of microRNAs using background-free molecular circuit

2020 ◽  
Vol 6 (4) ◽  
pp. eaay5952 ◽  
Author(s):  
Guillaume Gines ◽  
Roberta Menezes ◽  
Kaori Nara ◽  
Anne-Sophie Kirstetter ◽  
Valerie Taly ◽  
...  
Keyword(s):  
Regulation Of Gene Expression ◽  
Isothermal Amplification ◽  
Absorption Mechanism ◽  
Molecular Programming ◽  
Bodily Fluids ◽  
Microrna Detection ◽  
Nonspecific Amplification ◽  
Femtomolar Concentration ◽  
Accurate Quantification ◽  
Digital Counting

MicroRNAs, a class of transcripts involved in the regulation of gene expression, are emerging as promising disease-specific biomarkers accessible from tissues or bodily fluids. However, their accurate quantification from biological samples remains challenging. We report a sensitive and quantitative microRNA detection method using an isothermal amplification chemistry adapted to a droplet digital readout. Building on molecular programming concepts, we design a DNA circuit that converts, thresholds, amplifies, and reports the presence of a specific microRNA, down to the femtomolar concentration. Using a leak absorption mechanism, we were able to suppress nonspecific amplification, classically encountered in other exponential amplification reactions. As a result, we demonstrate that this isothermal amplification scheme is adapted to digital counting of microRNAs: By partitioning the reaction mixture into water-in-oil droplets, resulting in single microRNA encapsulation and amplification, the method provides absolute target quantification. The modularity of our approach enables to repurpose the assay for various microRNA sequences.

MicroRNA detection at femtomolar concentrations with isothermal amplification and a biological nanopore

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16124-16127 ◽  
Author(s):  
Haolin Zhang ◽  
Moe Hiratani ◽  
Kentaro Nagaoka ◽  
Ryuji Kawano
Keyword(s):  
Isothermal Amplification ◽  
Microrna Detection ◽  
Low Concentrations

This paper describes a method to detect ultra-low concentrations of nucleotides using isothermal amplification and a biological nanopore.

A ligation-based loop-mediated isothermal amplification (ligation-LAMP) strategy for highly selective microRNA detection

2016 ◽  
Vol 52 (86) ◽  
pp. 12721-12724 ◽  
Author(s):  
Wenfang Du ◽  
Mengmei Lv ◽  
Junjie Li ◽  
Ruqin Yu ◽  
Jianhui Jiang
Keyword(s):  
Isothermal Amplification ◽  
Lamp Method ◽  
Selective Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Microrna Detection

A novel ligation-based loop-mediated isothermal amplification (ligation-LAMP) method has been developed for sensitive and selective detection of microRNA.

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

2017 ◽  
Author(s):  
Bo Tian ◽  
Peter Svedlindh ◽  
Mattias Strömberg ◽  
Erik Wetterskog
Keyword(s):  
Magnetic Nanoparticles ◽  
Ferromagnetic Resonance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Rolling Circle Amplification ◽  
Resonance Field ◽  
Isothermal Amplification ◽  
Detection Sensitivity ◽  
Serum Samples ◽  
Rolling Circle

In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, <i>i.e.</i>, rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg<sub>2</sub>P<sub>2</sub>O<sub>7</sub> (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.<br>

The influence of SYBR Green I on isothermal amplification with Bst exo DNA polymerase.

Biomics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 268-273
Author(s):  
A.R. Gilvanov ◽  
A.R. Sakhabutdinova ◽  
A.V. Chemeris ◽  
R.R. Garafutdinov
Keyword(s):  
Dna Polymerase ◽  
Sybr Green ◽  
Isothermal Amplification ◽  
Sybr Green I
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