Quantitative generalized ratiometric fluorescence spectroscopy for turbid media based on probe encapsulated by biologically localized embedding

2016 ◽  
Vol 921 ◽  
pp. 38-45 ◽  
Author(s):  
Xiu-Fang Yan ◽  
Zeng-Ping Chen ◽  
Yin-Yin Cui ◽  
Yuan-Liang Hu ◽  
Ru-Qin Yu
Keyword(s):  
Fluorescence Spectroscopy ◽  
Turbid Media ◽  
Ratiometric Fluorescence

Quantitative Fluorescence Spectroscopy in Turbid Media: A Practical Solution to the Problem of Scattering and Absorption

2013 ◽  
Vol 85 (4) ◽  
pp. 2015-2020 ◽  
Author(s):  
Yao Chen ◽  
Zeng-Ping Chen ◽  
Jing Yang ◽  
Jing-Wen Jin ◽  
Juan Zhang ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Turbid Media ◽  
Practical Solution ◽  
Quantitative Fluorescence

Intrinsic fluorescence spectroscopy in turbid media: disentangling effects of scattering and absorption

2001 ◽  
Vol 40 (25) ◽  
pp. 4633 ◽  
Author(s):  
Markus G. Müller ◽  
Irene Georgakoudi ◽  
Qingguo Zhang ◽  
Jun Wu ◽  
Michael S. Feld
Keyword(s):  
Fluorescence Spectroscopy ◽  
Intrinsic Fluorescence ◽  
Turbid Media

Quantitative fluorescence spectroscopy in turbid media using fluorescence differential path length spectroscopy

2008 ◽  
Vol 13 (5) ◽  
pp. 054051 ◽  
Author(s):  
Arjen Amelink ◽  
Bastiaan Kruijt ◽  
Dominic J. Robinson ◽  
Henricus J. C. M. Sterenborg
Keyword(s):  
Fluorescence Spectroscopy ◽  
Path Length ◽  
Turbid Media ◽  
Quantitative Fluorescence

Fluorescence spectroscopy of turbid media: Autofluorescence of the human aorta

1989 ◽  
Vol 28 (20) ◽  
pp. 4286 ◽  
Author(s):  
Marleen Keijzer ◽  
Rebecca R. Richards-Kortum ◽  
Steven L. Jacques ◽  
Michael S. Feld
Keyword(s):  
Fluorescence Spectroscopy ◽  
Turbid Media ◽  
Human Aorta

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

Sign in / Sign up

Export Citation Format

Share Document