Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification

2016 ◽  
Vol 916 ◽  
pp. 112-119 ◽  
Author(s):  
Kenichiro Todoroki ◽  
Tatsuki Nakano ◽  
Yasuhiro Eda ◽  
Kaname Ohyama ◽  
Hideki Hayashi ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Temperature ◽  
Fluorescence Detection ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Magnetic Purification

scholarly journals Multiresidue Determination of Fluoroquinolones in Eggs

2000 ◽  
Vol 83 (6) ◽  
pp. 1306-1312 ◽  
Author(s):  
Marilyn J Schneider ◽  
Dan J Donoghue
Keyword(s):  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Small Volume ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Trace Enrichment ◽  
On Line ◽  
Phase Liquid ◽  
Multiresidue Determination

Abstract A multiresidue method was developed for the determination of fluoroquinolones in eggs. Extraction of eggs with ammoniacal acetonitrile was followed by liquid–liquid defatting, solvent evaporation, and redissolution in a small volume of buffer. The fluoroquinolones were further purified by on-line microdialysis, concentrated on a trace enrichment column, and separated by reversed-phase liquid chromatography with fluorescence detection. Norfloxacin (NOR), ciprofloxacin (CIP), and sarafloxacin (SAR) were extracted from fortified eggs over a range of 2–200 μg/kg, with recoveries of 65.7–78.9%, 65.6–77.1%, and 67.6–110%, respectively. Enrofloxacin (ENRO) was extracted over a range of 1–100 μg/kg, with recoveries of 71.5–86.7%, whereas desethylene ciprofloxacin (DCIP) and danofloxacin (DANO) were extracted over a range of 0.2–20 μg/kg, with recoveries of 68.7–90.7% and 76.0–93.8%, respectively. The limits of quantitation for the 6 fluoroquinolones were as follows: DCIP and DANO, 0.3 μg/kg; ENRO, 1 μg/kg; NOR and CIP, 2 μg/kg; and SAR, 3 μg/kg. Both SAR and ENRO incurred eggs were also successfully analyzed using this method.

Liquid-chromatographic study of fluorescent compounds in hemodialysate solutions.

1985 ◽  
Vol 31 (1) ◽  
pp. 127-130 ◽  
Author(s):  
A L Barnett ◽  
H Veening
Keyword(s):  
Acetic Acid ◽  
Liquid Chromatography ◽  
Fluorescence Detection ◽  
Reversed Phase ◽  
Chromatographic Study ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Liquid Chromatographic ◽  
Fluorescent Compounds ◽  
Indole 3 Acetic Acid

Abstract We have separated and identified three endogenous, naturally fluorescent substances in uremic hemodialysate by using reversed-phase liquid chromatography with fluorescence detection. Co-chromatography with authentic standards, monitoring peak shifts after enzymic treatment, and spectrofluorescence measurements were used to confirm the identity of indican, tryptophan, and indole-3-acetic acid. Concentrations of indican were about 1.5 those of tryptophan and considerably greater than those of indole-3-acetic acid in hemodialysate samples from 12 renal patients. These three compounds, as well as eight unidentified components, were consistently present in dialysate samples from each of the 12 patients.

Reversed-phase liquid chromatography on a microspherical carbon column at high temperature

1996 ◽  
Vol 728 (1-2) ◽  
pp. 189-194 ◽  
Author(s):  
Satoshi Yamaki ◽  
Toshiaki Isobe ◽  
Tsuneo Okuyama ◽  
Tomotaka Shinoda
Keyword(s):  
Liquid Chromatography ◽  
High Temperature ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid
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