scholarly journals Paper-based chromatic toxicity bioassay by analysis of bacterial ferricyanide reduction

2016 ◽  
Vol 910 ◽  
pp. 60-67 ◽  
Author(s):  
F. Pujol-Vila ◽  
N. Vigués ◽  
A. Guerrero-Navarro ◽  
S. Jiménez ◽  
D. Gómez ◽  
...  
Keyword(s):  
Toxicity Bioassay ◽  
Ferricyanide Reduction

Chromatic Toxicity Bioassay of Water through Bacterial Ferricyanide Reduction

2021 ◽  
Author(s):  
Maheen Shamim ◽  
Parus Shaikh ◽  
Zara Kanwal ◽  
Muhammad Noman Syed
Keyword(s):  
Visual Inspection ◽  
Cost Effective ◽  
Water Quality Assessment ◽  
Toxicity Bioassay ◽  
Toxic Agent ◽  
Ferricyanide Reduction ◽  
Weak Support ◽  
Long Period ◽  
General Toxicity ◽  
Water Purity

Abstract Water quality assessment demands a precise anatomization of specimens that comply with acclaimed water purity standards. Today, the growing number of toxicants and their synergistic consequences make it necessary to develop general toxicity assays able to examine and determine water pollution. Contemporary general toxicity methods hinder specimen analysis due to their prolonged operation protocols. Also, the equipment involved is very expensive that not everyone can afford it. In an effort to resolve these drawbacks, a quick and cost effective toxicity bioassay based on chromatic changes related to bacterial ferricyanide reduction is introduced here. E.coli cells (Model Bacteria) were stably confined on four supports: Cellulose-based Paper Discs, Silica 60, Polystyrene, and Acrylic Beads, which remained useful for a long period at -20ºC. Copper was used as a model toxic agent to perform Bioassay Assessment. Chromatic changes related to bacterial ferricyanide reduction were determined by visual inspection. Cellulose Paper Discs, Polystyrene, and Acrylic beads showed good results and better viability, while Silica 60 proved itself as a weak support and resulted in poor viability.

Fast and sensitive optical toxicity bioassay based on dual wavelength analysis of bacterial ferricyanide reduction kinetics

2015 ◽  
Vol 67 ◽  
pp. 272-279 ◽  
Author(s):  
F. Pujol-Vila ◽  
N. Vigués ◽  
M. Díaz-González ◽  
X. Muñoz-Berbel ◽  
J. Mas
Keyword(s):  
Dual Wavelength ◽  
Reduction Kinetics ◽  
Toxicity Bioassay ◽  
Ferricyanide Reduction

A Standard Primary Toxicity Bioassay Using Daphnia Magna as a Test Species

1977 ◽  
Vol 12 (1) ◽  
pp. 27-50 ◽  
Author(s):  
L.A. Behie ◽  
J.E. Zajic ◽  
D. Berk ◽  
R.J.P. Brouzes ◽  
V.A. Naish
Keyword(s):  
Statistical Analysis ◽  
Daphnia Magna ◽  
Biological Indicator ◽  
Pulp And Paper ◽  
Toxicity Bioassay ◽  
Simple Method ◽  
Test Species ◽  
Test Organisms ◽  
Dilution Water

Abstract Although Daphnia magna have been widely used in the determination of the toxicity of various substances, there are no reports in the literature that describe a rigorous bioassay method using this organism as a test species. The test described herein involves the standariza-tion of various important aspects of the method such as the age of the test organisms, and the dilution water used for the preparation of the various toxicant concentrations. Also described is a simple method for the statistical analysis of the results. The sensitivity of the proposed bioassay is demonstrated by determining the toxicity of various pulp and paper effluents. Finally, extensive bioassays were carried out simultaneously with rainbow trout and Daphnia magna indicating that Daphnia are as good a biological indicator of acute toxicity as fish.

A multi-pumping flow system for acute toxicity bioassay using the Vibrio fischeri bacteria

2014 ◽  
Vol 6 (18) ◽  
pp. 7367-7373 ◽  
Author(s):  
Andressa Adame ◽  
Fábio R. P. Rocha
Keyword(s):  
Acute Toxicity ◽  
Vibrio Fischeri ◽  
Flow System ◽  
Toxicity Bioassay ◽  
Fast Determination ◽  
Line Sample

Fast determination of acute toxicity using the V. fischeri bacteria, including in-line sample dilutions and conditioning.

Effect of heavy metals on the leaf disc ferricyanide reduction in cucumber

2009 ◽  
Vol 57 (3) ◽  
pp. 307-320
Author(s):  
G. Rabnecz ◽  
G. Záray ◽  
L. Lévai ◽  
F. Fodor
Keyword(s):  
Heavy Metals ◽  
Nutrient Solution ◽  
Chlorophyll Concentration ◽  
Leaf Disc ◽  
Transport Systems ◽  
Ferricyanide Reduction ◽  
Plasma Membrane Electron Transport ◽  
Significant Linear Correlation ◽  
Drastic Effect

The effect of heavy metals on the leaf plasma membrane electron transport systems was investigated in connection with the tissue Fe concentration in Fe-sufficient and Fe-deficient cucumber leaves. Ten M μPb in the nutrient solution inhibited leaf ferricyanide reduction by 20–26%, whereas 10 M μCd had a more drastic effect, with 80–83% inhibition. Ferricyanide reduction decreased by 14% when 1 mM Pb was applied in situ by vacuum infiltration into control leaf discs, whereas it decreased by 40% when 0.1 mM Cd was applied. Ferricyanide reduction was completely inhibited by 1 mM Cd. The ferricyanide reduction values were correlated with the heavy metal, Fe and chlorophyll concentrations in the leaves. A significant linear correlation was only found with the chlorophyll concentration. The data suggest that there are also direct effects on membranebound reductases, but these are of less significance. Using differentially Fe-deficient plants (grown with 0 to 300 nM Fe in the nutrient solution), a chlorophyll concentration of 0.9–1.0 mg g −1 fresh weight was estimated as the threshold for achieving the ferricyanide reduction levels found in the controls.

Effects of two diluents in the Microtox® toxicity bioassay with marine sediments

Chemosphere ◽  
2004 ◽  
Vol 54 (5) ◽  
pp. 679-687 ◽  
Author(s):  
Fulvio Onorati ◽  
Mauro Mecozzi
Keyword(s):  
Marine Sediments ◽  
Toxicity Bioassay
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