Aptamers-based sandwich assay for silver-enhanced fluorescence multiplex detection

2016 ◽  
Vol 905 ◽  
pp. 149-155 ◽  
Author(s):  
Ying Wang ◽  
Hui Li ◽  
Danke Xu
Keyword(s):  
Multiplex Detection ◽  
Enhanced Fluorescence ◽  
Sandwich Assay

A convenient sandwich assay of thrombin in biological media using nanoparticle-enhanced fluorescence polarization

2014 ◽  
Vol 56 ◽  
pp. 231-236 ◽  
Author(s):  
Qiaoli Yue ◽  
Tongfei Shen ◽  
Lei Wang ◽  
Shuling Xu ◽  
Haibo Li ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Enhanced Fluorescence ◽  
Sandwich Assay ◽  
Biological Media

Enhanced-Fluorescence of a Dye on DNA- assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy

2017 ◽  
Author(s):  
Pedro M. R. Paulo ◽  
David Botequim ◽  
Agnieszka Jóskowiak ◽  
Sofia Martins ◽  
Duarte M. F. Prazeres ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Emission ◽  
Directed Assembly ◽  
Plasmon Mode ◽  
Correlation Spectroscopy ◽  
Enhanced Fluorescence ◽  
Detection Volume ◽  
Relaxation Component ◽  
Good Agreement ◽  
Confocal Detection

<div> <div> <div> <p>We have employed DNA-directed assembly to prepare dimers of gold nanoparticles and used their longitudinally coupled plasmon mode to enhance the fluorescence emission of an organic red-emitting dye, Atto-655. The plasmon- enhanced fluorescence of this dye using dimers of 80 nm particles was measured at single molecule detection level. The top enhancement factors were above 1000-fold in 71% of the dimers within a total of 32 dimers measured, and, in some cases, they reached almost 4000-fold, in good agreement with model simulations. Additionally, fluorescence lifetime correlation analysis enabled the separation of enhanced from non-enhanced emission simultaneously collected in our confocal detection volume. This approach allowed us to recover a short relaxation component exclusive to enhanced emission that is attributed to the interaction of the dye with DNA in the interparticle gaps. </p> </div> </div> </div>

Autonomous Kit for Multiplex Detection of Antibodies to Infectious Diseases Causes

2018 ◽  
Vol 34 (5) ◽  
pp. 57-66
Author(s):  
A.G. Poltavchenko ◽  
◽  
O.V. Nechitailo ◽  
P.V. Filatov ◽  
A.V. Yorsh
Keyword(s):  
Infectious Diseases ◽  
Multiplex Detection

BSA Au Clusters as a Probe for Enhanced Fluorescence Detection Using Multipulse Excitation Scheme

2014 ◽  
Vol 14 (13) ◽  
pp. 1139-1144 ◽  
Author(s):  
Sangram Raut ◽  
Ryan Rich ◽  
Rafal Fudala ◽  
R. Kokate ◽  
J.D. Kimball ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Enhanced Fluorescence ◽  
Excitation Scheme ◽  
Au Clusters

scholarly journals Evaluation of Immuno-Rolling Circle Amplification for Multiplex Detection and Profiling of Antigen-Specific Antibody Isotypes

2021 ◽  
Vol 93 (15) ◽  
pp. 6169-6177
Author(s):  
Sara Horta ◽  
Felix Neumann ◽  
Shu-hao Yeh ◽  
Christoffer Mattsson Langseth ◽  
Kadri Kangro ◽  
...  
Keyword(s):  
Specific Antibody ◽  
Rolling Circle Amplification ◽  
Multiplex Detection ◽  
Rolling Circle ◽  
Antibody Isotypes
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