Sensitivity improvement of a sandwich-type ELISA immunosensor for the detection of different prostate-specific antigen isoforms in human serum using electrochemical impedance spectroscopy and an ordered and hierarchically organized interfacial supramolecular architecture

2016 ◽  
Vol 902 ◽  
pp. 97-106 ◽  
Author(s):  
Gabriela Guadalupe Gutiérrez-Zúñiga ◽  
José Luis Hernández-López
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Human Serum ◽  
Prostate Specific Antigen ◽  
Electrochemical Impedance ◽  
Specific Antigen ◽  
Supramolecular Architecture ◽  
Sandwich Type ◽  
Sensitivity Improvement

scholarly journals Impedance-Based Miniaturized Biosensor for Ultrasensitive and Fast Prostate-Specific Antigen Detection

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Ganna Chornokur ◽  
Sunil K. Arya ◽  
Catherine Phelan ◽  
Richard Tanner ◽  
Shekhar Bhansali
Keyword(s):  
Monoclonal Antibodies ◽  
Electrochemical Impedance Spectroscopy ◽  
Detection Limit ◽  
Impedance Spectroscopy ◽  
Prostate Specific Antigen ◽  
Electrochemical Impedance ◽  
Point Of Care ◽  
Specific Antigen ◽  
Nonspecific Binding ◽  
Order Of Magnitude

This paper reports the successful fabrication of an impedance-based miniaturized biosensor and its application for ultrasensitive Prostate-Specific Antigen (PSA) detection in standard and real human plasma solution, spiked with different PSA concentrations. The sensor was fabricated using photolithographic techniques, while monoclonal antibodies specific to human PSA were used as primary capture antibodies. Electrochemical impedance spectroscopy (EIS) was employed as a detection technique. The sensor exhibited a detection limit of 1 pg/ml for PSA with minimal nonspecific binding (NSB). This detection limit is an order of magnitude lower than commercial PSA ELISA assays available on the market. The sensor can be easily modified into an array for the detection of other biomolecules of interest, enabling accurate, ultrasensitive, and inexpensive point-of-care sensing technologies.

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