Homogeneous single-label tyrosine kinase activity assay for high throughput screening

2015 ◽  
Vol 897 ◽  
pp. 96-101 ◽  
Author(s):  
Natalia Tong-Ochoa ◽  
Kari Kopra ◽  
Markku Syrjänpää ◽  
Nicolas Legrand ◽  
Harri Härmä
Keyword(s):  
Tyrosine Kinase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
Activity Assay ◽  
Tyrosine Kinase Activity ◽  
Kinase Activity Assay

A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

2016 ◽  
Vol 52 (81) ◽  
pp. 12112-12115 ◽  
Author(s):  
Jieon Lee ◽  
Il-Soo Park ◽  
Ginam Park ◽  
Kyukwang Cho ◽  
Hee-Sung Park ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Protein Kinase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
Kinase Inhibitors ◽  
Protein Kinase Inhibitors ◽  
Protein Kinase Activity ◽  
Activity Assay ◽  
Kinase Activity Assay

We present a new platform for multiplexed protein kinase activity assay using TiO2decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening.

Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

2014 ◽  
Vol 50 (90) ◽  
pp. 13960-13962 ◽  
Author(s):  
Zhenzhen Deng ◽  
Mingliang Ye ◽  
Yangyang Bian ◽  
Zheyi Liu ◽  
Fangjie Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Kinase Activity ◽  
Maldi Ms ◽  
Time Dependent ◽  
Activity Assay ◽  
Dimethyl Labeling ◽  
Labeling Approach ◽  
Kinase Activity Assay

A multiplex isotope dimethyl labeling approach allowed MALDI MS to monitor the time dependent consumption of substrates and generation of products in one spot.

scholarly journals High-Throughput Tyrosine Kinase Activity Profiling Identifies FAK as a Candidate Therapeutic Target in Ewing Sarcoma

2013 ◽  
Vol 73 (9) ◽  
pp. 2873-2883 ◽  
Author(s):  
Brian D. Crompton ◽  
Anne L. Carlton ◽  
Aaron R. Thorner ◽  
Amanda L. Christie ◽  
Jinyan Du ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
High Throughput ◽  
Ewing Sarcoma ◽  
Therapeutic Target ◽  
Kinase Activity ◽  
Tyrosine Kinase Activity ◽  
Activity Profiling
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