A versatile label-free and signal-on electrochemical biosensing platform based on triplex-forming oligonucleotide probe

2015 ◽  
Vol 890 ◽  
pp. 91-97 ◽  
Author(s):  
Xiuzhong Wang ◽  
Aiwen Jiang ◽  
Ting Hou ◽  
Feng Li
Keyword(s):  
Oligonucleotide Probe ◽  
Label Free ◽  
Electrochemical Biosensing ◽  
Triplex Forming Oligonucleotide

scholarly journals Utilising Commercially Fabricated Printed Circuit Boards as an Electrochemical Biosensing Platform

Micromachines ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 793
Author(s):  
Uroš Zupančič ◽  
Joshua Rainbow ◽  
Pedro Estrela ◽  
Despina Moschou
Keyword(s):  
Printed Circuit Boards ◽  
Electrochemical Sensors ◽  
Cost Effective ◽  
Electrochemical Sensing ◽  
Label Free ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Sensing Applications ◽  
Electrochemical Biosensing ◽  
Pre Treatment

Printed circuit boards (PCBs) offer a promising platform for the development of electronics-assisted biomedical diagnostic sensors and microsystems. The long-standing industrial basis offers distinctive advantages for cost-effective, reproducible, and easily integrated sample-in-answer-out diagnostic microsystems. Nonetheless, the commercial techniques used in the fabrication of PCBs produce various contaminants potentially degrading severely their stability and repeatability in electrochemical sensing applications. Herein, we analyse for the first time such critical technological considerations, allowing the exploitation of commercial PCB platforms as reliable electrochemical sensing platforms. The presented electrochemical and physical characterisation data reveal clear evidence of both organic and inorganic sensing electrode surface contaminants, which can be removed using various pre-cleaning techniques. We demonstrate that, following such pre-treatment rules, PCB-based electrodes can be reliably fabricated for sensitive electrochemical biosensors. Herein, we demonstrate the applicability of the methodology both for labelled protein (procalcitonin) and label-free nucleic acid (E. coli-specific DNA) biomarker quantification, with observed limits of detection (LoD) of 2 pM and 110 pM, respectively. The proposed optimisation of surface pre-treatment is critical in the development of robust and sensitive PCB-based electrochemical sensors for both clinical and environmental diagnostics and monitoring applications.

Label-free and facile electrochemical biosensing using carbon nanotubes for malondialdehyde detection

The Analyst ◽  
2013 ◽  
Vol 138 (11) ◽  
pp. 3131 ◽  
Author(s):  
Ling Yuan ◽  
Yaqian Lan ◽  
Min Han ◽  
Jianchun Bao ◽  
Wenwen Tu ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Label Free ◽  
Electrochemical Biosensing

scholarly journals Label-free electrochemical biosensing of small-molecule inhibition on O-GlcNAc glycosylation

2017 ◽  
Vol 95 ◽  
pp. 94-99 ◽  
Author(s):  
Yu Yang ◽  
Yuxin Gu ◽  
Bin Wan ◽  
Xiaomin Ren ◽  
Liang-Hong Guo
Keyword(s):  
Small Molecule ◽  
Label Free ◽  
Small Molecule Inhibition ◽  
Electrochemical Biosensing

scholarly journals Label-Free Electrochemical Biosensors for the Determination of Flaviviruses: Dengue, Zika, and Japanese Encephalitis

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4600 ◽  
Author(s):  
Ekaterina Khristunova ◽  
Elena Dorozhko ◽  
Elena Korotkova ◽  
Bohumil Kratochvil ◽  
Vlastimil Vyskocil ◽  
...  
Keyword(s):  
Japanese Encephalitis ◽  
Electrode Materials ◽  
Biological Fluids ◽  
Research Progress ◽  
Immunological Methods ◽  
Label Free ◽  
Viral Pathogens ◽  
Electrochemical Biosensing ◽  
Wide Range

A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.

Label-free optical bifunctional oligonucleotide probe for homogeneous amplification detection of disease markers

2011 ◽  
Vol 29 (1) ◽  
pp. 66-75 ◽  
Author(s):  
Kejun Feng ◽  
Li-Ping Qiu ◽  
Yifeng Yang ◽  
Zai-Sheng Wu ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Oligonucleotide Probe ◽  
Label Free ◽  
Disease Markers
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