An electrochemical aptasensor for thrombin detection based on the recycling of exonuclease III and double-stranded DNA-templated copper nanoparticles assisted signal amplification

2015 ◽  
Vol 860 ◽  
pp. 23-28 ◽  
Author(s):  
Jing Zhao ◽  
Meiling Xin ◽  
Ya Cao ◽  
Yongmei Yin ◽  
Yongqian Shu ◽  
...  
Keyword(s):  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Aptasensor ◽  
Exonuclease Iii ◽  
Double Stranded Dna

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

BMC Chemistry ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3′ end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3′–5′ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0 × 10−3–1000 ng mL−1 with a low limit of detection of 0.15 pg mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Novel ultrasensitive homogeneous electrochemical aptasensor based on dsDNA-templated copper nanoparticles for the detection of ractopamine

2017 ◽  
Vol 5 (1) ◽  
pp. 53-61 ◽  
Author(s):  
Feifan Sheng ◽  
Xiaojun Zhang ◽  
Guangfeng Wang
Keyword(s):  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Ultrasensitive Detection ◽  
Electrochemical Aptasensor ◽  
Hairpin Dna

Herein, we describe a novel homogenous electrochemical aptasensor for the ultrasensitive detection of ractopamine (RAC) based on the signal amplification of a hairpin DNA cascade amplifier (HDCA) and electrocatalysis of dsDNA-templated copper nanoparticles.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Exonuclease III-assisted signal amplification strategy for sensitive fluorescence detection of polynucleotide kinase based on poly(thymine)-templated copper nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (22) ◽  
pp. 6689-6697 ◽  
Author(s):  
Han Zhao ◽  
Ying Yan ◽  
Mingjian Chen ◽  
Tingting Hu ◽  
Kefeng Wu ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Copper Nanoparticles ◽  
Signal Amplification ◽  
Exonuclease Iii ◽  
Fluorescent Method ◽  
Polynucleotide Kinase ◽  
Amplification Strategy

A facile fluorescent method has been developed for polynucleotide kinase detection based on copper nanoparticles and exonuclease III-assisted signal amplification.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

Ultrasensitive biosensing platform based on layered vanadium disulfide–graphene composites coupling with tetrahedron-structured DNA probes and exonuclease III assisted signal amplification

2015 ◽  
Vol 3 (41) ◽  
pp. 8180-8187 ◽  
Author(s):  
Ke-Jing Huang ◽  
Yu-Jie Liu ◽  
Qiu-Fen Zhai
Keyword(s):  
Signal Amplification ◽  
Dna Probes ◽  
Electrochemical Aptasensor ◽  
Exonuclease Iii

An electrochemical aptasensor is developed to sensitively detect PDGF-BB based on vanadium disulfide–graphene composites and exonuclease III signal amplification.

scholarly journals A novel electrochemical aptasensor for fumonisin B1 determination using DNA and exonuclease-I as signal amplification strategy

2019 ◽  
Author(s):  
Min Wei ◽  
Fei Zhao ◽  
Shuo Feng ◽  
Huali Jin
Keyword(s):  
Incubation Time ◽  
Electrode Surface ◽  
Signal Amplification ◽  
Limit Of Detection ◽  
Fumonisin B1 ◽  
Electrochemical Aptasensor ◽  
Experimental Conditions ◽  
Exonuclease I ◽  
Double Stranded Dna ◽  
Amplification Strategy

Abstract In this work, using DNA and exonuclease-I (Exo-I) as signal amplification strategy, a novel and facile electrochemical aptasensor was constructed for fumonisin B1 (FB1) detection. The G-rich complementary DNA (cDNA) was immobilized onto the electrode surface. Then, aptamer of FB1 was hybridized with cDNA to form double-stranded DNA. In the absence of FB1, double-stranded DNA and G-rich cDNA on the electrode surface promoted effectively methylene blue (MB) enrichment and amplified the initial electrochemical response. In the presence of FB1, the combination of aptamer and FB1 led to the release of aptamer from the electrode surface and the expose of 3' end of single-stranded cDNA. When Exo-I was added onto the electrode surface, the single-stranded cDNA was degraded in the 3’-5’ direction. The decrease of double-stranded DNA and G-rich cDNA resulted in the less access of MB to the electrode surface, which decreased the electrochemical signal. The experimental conditions including incubation time of FB1, the amount of Exo-I and incubation time of Exo-I were optimized. Under the optimal conditions, the linear relationship between the change of peak current and the logarithmic concentration of FB1 was observed in the range of 1.0×10-3-1000ng·mL−1 with a low limit of detection of 0.15 pg·mL−1. The experimental results showed that the prepared aptasensor had acceptable specificity, reproducibility, repeatability and stability. Therefore, this proposed aptasensor has a potential application in the food safety detection.

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