High loading MnO 2 nanowires on graphene paper: Facile electrochemical synthesis and use as flexible electrode for tracking hydrogen peroxide secretion in live cells

2015 ◽  
Vol 853 ◽  
pp. 200-206 ◽  
Author(s):  
Shuang Dong ◽  
Jiangbo Xi ◽  
Yanan Wu ◽  
Hongwei Liu ◽  
Chaoyang Fu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Synthesis ◽  
High Loading ◽  
Live Cells ◽  
Flexible Electrode ◽  
Graphene Paper

Electrochemical synthesis of macroporous zinc oxide layers by employing hydrogen peroxide as oxygen precursor

2008 ◽  
Vol 205 (10) ◽  
pp. 2365-2370 ◽  
Author(s):  
Daniel Ramirez ◽  
Philip Bartlett ◽  
Mamdouh Abdelsalam ◽  
Humberto Gomez ◽  
Daniel Lincot
Keyword(s):  
Hydrogen Peroxide ◽  
Zinc Oxide ◽  
Electrochemical Synthesis ◽  
Oxide Layers

SnO2-supported single metal atoms: a bifunctional catalyst for the electrochemical synthesis of H2O2

2021 ◽  
Author(s):  
Santiago Jimenez-Villegas ◽  
Sara R. Kelly ◽  
Samira Siahrostami
Keyword(s):  
Hydrogen Peroxide ◽  
Electrochemical Synthesis ◽  
Bifunctional Catalyst ◽  
Metal Atoms

SnO2 supported single metal atoms offer unique bifunctional catalyst materials for electrochemical synthesis of hydrogen peroxide via 2e-ORR and 2e-WOR.

scholarly journals Construction of a Nanosensor for Non-Invasive Imaging of Hydrogen Peroxide Levels in Living Cells

Biology ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 430
Author(s):  
Amreen ◽  
Hayssam M. Ali ◽  
Mohammad Ahmad ◽  
Mohamed Z. M. Salem ◽  
Altaf Ahmad
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Mammalian Cells ◽  
Dynamic Range ◽  
Resonance Energy ◽  
Living Cells ◽  
Stress Conditions ◽  
Live Cells ◽  
Cell Physiology ◽  
Broad Dynamic Range

Hydrogen peroxide (H2O2) serves fundamental regulatory functions in metabolism beyond the role as damage signal. During stress conditions, the level of H2O2 increases in the cells and causes oxidative stress, which interferes with normal cell growth in plants and animals. The H2O2 also acts as a central signaling molecule and regulates numerous pathways in living cells. To better understand the generation of H2O2 in environmental responses and its role in cellular signaling, there is a need to study the flux of H2O2 at high spatio–temporal resolution in a real-time fashion. Herein, we developed a genetically encoded Fluorescence Resonance Energy Transfer (FRET)-based nanosensor (FLIP-H2O2) by sandwiching the regulatory domain (RD) of OxyR between two fluorescent moieties, namely ECFP and mVenus. This nanosensor was pH stable, highly selective to H2O2, and showed insensitivity to other oxidants like superoxide anions, nitric oxide, and peroxynitrite. The FLIP-H2O2 demonstrated a broad dynamic range and having a binding affinity (Kd) of 247 µM. Expression of sensor protein in living bacterial, yeast, and mammalian cells showed the localization of the sensor in the cytosol. The flux of H2O2 was measured in these live cells using the FLIP-H2O2 under stress conditions or by externally providing the ligand. Time-dependent FRET-ratio changes were recorded, which correspond to the presence of H2O2. Using this sensor, real-time information of the H2O2 level can be obtained non-invasively. Thus, this nanosensor would help to understand the adverse effect of H2O2 on cell physiology and its role in redox signaling.

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