Combination of micropreparative solution isoelectric focusing and high-performance liquid chromatography for differentiation of biofilm-positive and biofilm-negative Candida parapsilosis group from vascular catheter

2014 ◽  
Vol 812 ◽  
pp. 243-249 ◽  
Author(s):  
Marie Vykydalová ◽  
Marie Horká ◽  
Filip Růžička ◽  
Filip Duša ◽  
Dana Moravcová ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Candida Parapsilosis

Usefulness of Cation Exchange High Performance Liquid Chromatography as a Testing Procedure

PEDIATRICS ◽  
1989 ◽  
Vol 83 (5) ◽  
pp. 849-851
Author(s):  
Titus H. J. Huisman
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Cation Exchange ◽  
Cellulose Acetate ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Testing Procedure ◽  
Cellulose Acetate Electrophoresis ◽  
Blood Samples ◽  
Testing Procedures

Testing of cord blood or newborn blood samples for hemoglobin abnormalities should include clinically important hemoglobinopathies other than sickle cell anemia (SS), such as SC, SD, SO, S-β- thalassemia (thal), EE, SE, and α-thal, and should place the quality of the testing procedures (ie, accuracy of diagnosis) above quantity (ie, number of samples tested over a given period). There is no single method available that is suitable for the identification of each of the numerous abnormalities; thus, at least two, and often more than two, procedures must be used to reach a definitive diagnosis. For this reason, blood samples collected in vacutainers with ethylenediaminetetraacetic acid as anticoagulant are preferred to those collected on filter papers. The latter approach also has the disadvantage that, under a less than optimal transport system, hemoglobin is readily modified (oxidation, glycosylation, protein-protein interaction), producting extra bands or peaks in electrophoretic or chromatographic separations that interfere with an appropriate identification of various genetically determined hemoglobin variants. In our laboratories, in which hemoglobin identification has been routine for more than 25 years, we consider the following procedures acceptable primary testing methods: starch gel electrophoresis at pH 8.9, cellulose acetate electrophoresis at pH 8.5 to 8.9, isoelectric focusing, and fast cation exchange high performance liquid chromatography (HPLC). The following five methods are excellent confirmatory testing procedures: citrate agar electrophoresis at pH 6.1, cation or anion exchange macrochromatography, isoelectric focusing, cation exchange HPLC, and immunologic procedures. Combinations of these techniques will often lead to acceptable data, and the general approach followed in our institute is given in Fig 1. Cellulose acetate electrophoresis at alkaline pH is still the primary testing procedure, and citrate agar electrophoresis at pH 6.1 and micro-HPLC procedures are the main confirmatory methods.

Effect of aspirin on determinations of glycosylated hemoglobin.

1983 ◽  
Vol 29 (3) ◽  
pp. 466-469 ◽  
Author(s):  
D M Nathan ◽  
T B Francis ◽  
J L Palmer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Glycosylated Hemoglobin ◽  
Apparent Increase ◽  
In Vitro Effects ◽  
Aspirin Ingestion

Abstract We investigated the in vivo and in vitro effects of aspirin on several clinical assays of glycosylated hemoglobin. Acetylation of hemoglobin falsely increased the glycosylated hemoglobin fraction measured by "high-performance" liquid chromatography and electrophoresis, but isoelectric focusing and colorimetric techniques differentiated between acetylated and glycosylated fractions. Aspirin ingestion may result in an apparent increase in glycosylated hemoglobin measured with common clinical assays.

Analysis of enzymatically released peptides by revese-phase high performance liquid chromatography from picomole amounts of apolipoproteins separated on polyacrylamide isoelectric focusing gels

1982 ◽  
Vol 60 (8) ◽  
pp. 790-797 ◽  
Author(s):  
Leo V. Pereira ◽  
Peter J. Dolphin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Acid Precipitation ◽  
Comprehensive Analysis ◽  
Low Molecular Weight ◽  
Peptide Analysis ◽  
Reverse Phase ◽  
Structural Differences

Methods were developed for the peptide analysis of individual isoproteins of human apolipoproteins separated on urea–polyacrylamide isoelectric focusing (IEF) gels. After IEF the proteins were fixed in the gel matrix by trichloroacetic acid precipitation. Low molecular weight contaminants, including ampholytes, were removed and the proteins were chemically desialylated. Enzymatic digestions with L-1-tosyl-2-phenylethylchloromethyl ketone – trypsin, chymotrypsin, or with thermolysin were accomplished within the gel matrix. The proteolytically released peptides were analyzed by reverse-phase high performance liquid chromatography. These methods facilitated the comprehensive analysis of protein structural differences between individual isoproteins of apolipoproteins in duplicate with as little as 1–2 nmol of each isoprotein, without the use of radiolabels. Human apolipoproteins A-I, C, and E were analysed by these methods.

Comparison of an Isoelectric Focusing Technique and High-Performance Liquid Chromatography for Determination of Fetal Hemoglobin Levels

1991 ◽  
Vol 96 (1) ◽  
pp. 109-110 ◽  
Author(s):  
Enid F. Gilbert-Barness ◽  
Katherine S. Kenison ◽  
Earl Shrago ◽  
Terry L. Spennetta ◽  
Gary G. Giulian
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
High Performance ◽  
Fetal Hemoglobin
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