Synthesis and application of ionic liquid matrices (ILMs) for effective pathogenic bacteria analysis in matrix assisted laser desorption/ionization (MALDI-MS)

2013 ◽  
Vol 767 ◽  
pp. 104-111 ◽  
Author(s):  
Hani Nasser Abdelhamid ◽  
Judy Gopal ◽  
Hui-Fen Wu
Keyword(s):  
Ionic Liquid ◽  
Pathogenic Bacteria ◽  
Laser Desorption ◽  
Maldi Ms ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Liquid Matrices ◽  
Ionic Liquid Matrices ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals New Strategy to Preserve Phosphate by Ionic Liquid Matrices in Matrix-Assisted Laser Desorption/Ionization: A Case of Adenosine Nucleotides

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1217
Author(s):  
Chih-Hao Lin ◽  
Chuping Lee ◽  
Yu-Cheng Wu ◽  
I-Chung Lu
Keyword(s):  
Ionic Liquid ◽  
Detection Efficiency ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Liquid Matrices ◽  
Ionic Liquid Matrices ◽  
Adenosine Nucleotides ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Adenosine -5′-triphosphate (ATP) plays a valuable role in metabolic activity to produce adequate energy in a biosystem. A high ATP/AMP ratio has a correlation with diabetes that induces suppression of AMP-activated protein kinase (AMPK). Matrix-assisted laser desorption/ionization (MALDI)–mass spectrometry (MS) has outstanding potential in determining the ratio of several types of adenosine phosphates in a sample to rapidly understand the primary energy transfer in metabolism. Although MALDI is viewed as a soft ionization technique for MS analysis, excess photon energy might crack the phosphate bonds leading to misinterpretation of the ATP level. In this work, ionic liquid matrices (ILMs) were employed to reduce fragmentation and increase the detection efficiency during the MALDI process. This study demonstrated for the first time that 2,5-dihydroxybenzoic acid pyridine (DHBP) is one of the most effective matrices for further quantitative analysis of adenosine nucleotides. This systematic screening of ILMs also enhances the fundamental understanding of MALDI.

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